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1.
Arch Virol ; 163(4): 1105, 2018 04.
Article in English | MEDLINE | ID: mdl-29399746

ABSTRACT

Unfortunately, the family name of the co-author Roberto Cruz-Flores was incorrectly published in the original publication and corrected here by this correction. The original article has been corrected.

2.
Arch Virol ; 163(4): 1101-1104, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29327235

ABSTRACT

Bacteriophages are recognized as major mortality agents of microbes, among them intracellular marine rickettsiales-like bacteria. Recently, a phage hyperparasite of Candidatus Xenohaliotis californiensis (CXc) has been described. This bacterium is considered the causal agent of Withering Syndrome (WS) which is a chronic and potentially lethal disease of abalone species from California, USA and the peninsula of Baja California, Mexico. This hyperparasite which infects CXc could be used as a biocontrol agent for WS. Therefore, it is necessary to obtain genomic information to characterize this phage. In this study, the first complete genome sequence of a novel phage, Xenohaliotis phage (pCXc) was determined. The complete genome of pCXc from red abalone (Haliotis rufescens) is 35,728 bp, while the complete genome of pCXc from yellow abalone (Haliotis corrugata) is 35,736 bp. Both phage genomes consist of double-stranded DNA with a G + C content of 38.9%. In both genomes 33 open reading frames (ORFs) were predicted. Only 10 ORFs encode proteins that have identifiable functional homologues. These 10 ORFs were classified by function, including structural, DNA replication, DNA packaging, nucleotide transport and metabolism, life cycle regulation, recombination and repair, and additional functions. A PCR method for the specific detection of pCXc was developed. This information will help to understand a new group of phages that infect intracellular marine rickettsiales-like bacteria in mollusks.


Subject(s)
Anaplasmataceae/virology , Bacteriophages/genetics , Gastropoda/microbiology , Genome, Viral , RNA, Double-Stranded/genetics , RNA, Viral/genetics , Animals , Biological Control Agents , Chromosome Mapping , Gene Expression , Open Reading Frames , Whole Genome Sequencing
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