ABSTRACT
Plant-mediated biosynthesis of nanoparticles is a green method that allows synthesis in one-pot process. Synthesis of gold nanoparticles with plant extracts has gained interest in the field of biomedicine due to its variety of applications. This study presents the synthesis via green chemistry of gold nanoparticles (AuNPs) using the methanol extract of Moringa oleifera seeds. The AuNPs were synthesized at room temperature. UV-Vis spectroscopy confirmed the formation of AuNPs by identifying the surface plasmon resonance located at 546 nm. TEM analysis shows spherical nanoparticles. FTIR analysis demonstrated the presence of specific bioactive molecules responsible for the Au3+ ion reduction process. The antioxidant activity of the nanoparticles was evaluated on the stabilization of the DPPH radical (1,1-diphenyl-2-picrylhydrazyl, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl). The antimicrobial activity analysis was developed by broth microdilution method at different concentrations against Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration were 400 µg/mL and 200 µg/mL, respectively. A549 lung cancer cell proliferation was measured according to the MTT protocol, indicating a dose-dependent response and a IC50 of 163.9 ± 13.27 µg/mL. The AuNPs synthesized using M. oleifera seeds showed promise as active materials for antimicrobial or anticancer products.
Subject(s)
Anti-Bacterial Agents , Antioxidants , Gold , Green Chemistry Technology , Lung Neoplasms , Metal Nanoparticles , Moringa oleifera , Plant Extracts , Seeds , Staphylococcus aureus , Moringa oleifera/chemistry , Metal Nanoparticles/chemistry , Gold/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Plant Extracts/pharmacology , Plant Extracts/chemistry , Seeds/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Green Chemistry Technology/methods , Humans , Staphylococcus aureus/drug effects , Lung Neoplasms/drug therapy , Microbial Sensitivity Tests , Escherichia coli/drug effects , A549 CellsABSTRACT
The periodontal condition of 30 type II non-insulin-dependent diabetic patients (NIDDM) was evaluated and compared to that of 30 non-diabetic patients (age range, 30-77 years). Glycosylated hemoglobin and fasting glucose tests were used to measure the metabolic control of the patients, and the BANA (N-benzoyl-DL-arginine-naphthylamide) test was used to analyze subgingival microbiota. At the end of the study, the diabetic group was subdivided into three groups according to the degree of diabetic control: controlled, moderately controlled and poorly controlled. The non-parametric chi square test was used for statistical analyses: BANA test--no statistically significant differences were found between the two groups when analyzed as a whole. When the diabetic patients were subdivided and compared to the control group, significance was detected (P < 0.01) in the total frequencies of the BANA scores (negative, moderately positive and positive: 1, 2, and 3, respectively) in the 5-, 6-, and 7-mm pockets and also in the 4-mm pockets in the poorly controlled group.
