[Trifluoperazine's action on fibroblast proliferation in cell cultures. Comparison of different doses]. / Acción de la trifluoperazina sobre la proliferación fibroblástica en cultivos celulares. Comparación de diferentes dosis.

PURPOSE: To evaluate the effect of different doses of trifluoperazine (TFP) on fibroblastic proliferation in cell cultures of sclera and conjunctiva. METHODS: 24 brown rabbits were operated on for non-protected sclerectomy and were divided into four groups. Group 1: Subconjunctival injection of 0.5 ml of balanced saline solution (BSS) 24 h before surgery. Group 2: Intraoperative TFP 10(-2)M for five minutes on the sclera in experimental filtrating surgery. Group 3: Subconjunctival injection of 0.5 ml of TFP 10(-3) M 24 h before surgery. Group 4: Subconjunctival injection of 0.5 ml of TFP 10-4M 24 h before surgery. Conjunctiva and sclera tissue samples were taken 1 hour after surgery in the treated area and at 90 degrees from it. All of the samples were cultured in DMEM with 20% fetal calf serum. The presence of atypical cells and fibroblastic growth was assessed at 3, 6, 9 and 12 days. RESULTS: There were statistically significant differences in all the groups and in the times compared to the control group. In the treated conjunctiva, differences were found between the subconjunctiva 10(-3) M and the intraoperative 10(-2) M on the 3rd and 6th days, between the preoperative 10-2 and subconjunctiva 10(-3) M and 10(-4) after the 6th day. In conjunctiva at 90 degrees, there were significant differences between preoperative 10-2 M and subconjunctiva 10-3 after day 6, between the preoperative 10-2 M and subconjunctiva 10(-4) on days 9 and 12. There were no significant differences among the different treatments in the sclera samples, but there were differences between all them and the control group. CONCLUSIONS: TFP at a dose of 10(-2) and 10(-4) TFP has an inhibitory effect on cellular proliferation in vitro in the rabbit sclera and conjunctiva. All the doses used produce a significant inhibition of cellular proliferation without producing significant disorders in the cellular morphology.

[Predictive value of the pattern-electroretinogram in glaucoma]. / Valor predictivo del pattern-electrorretinograma en el glaucoma.

PURPOSE: To determine the ability of pattern-electroretinogram (PERG) to discriminate individual evolution of glaucomatous visual field losses in glaucoma suspects. METHODS: We studied 151 eyes of 78 GS, classified depending on their risk to develop glaucomatous damage. The initial visual field was normal. Those eyes showing at least 2 reduced amplitudes in the PERG components (P50, N95 and NP) were considered pathologic. The mean follow-up was 30 months (range: 13-50 months); the VF was evaluated at the end of the study. RESULTS: At the beginning of the study the PERG was pathologic in 65 eyes (43%); in 45 of these eyes (69%) the visual field was pathologic at the end of the study. The positive predictive value of the PERG of visual field glaucomatous changes was 69% and the negative predictive value was 80%. The N95 amplitude showed the best predictive value, with 100% and 77% in the high and medium risk groups, respectively. CONCLUSIONS: The PERG study can discriminate the glaucoma suspects that will develop visual field glaucomatous losses.