Zonal Chemical Signal Pathways Mediating Floral Induction in Apple.

Phytohormones that trigger or repress flower meristem development in apple buds are thought to be locally emitted from adjacent plant tissues, including leaves and fruitlets. The presence of fruitlets is known to inhibit adjacent buds from forming flowers and thus fruits. The resulting absence of fruitlets the following season restores flower-promoting signalling to the new buds. The cycle can lead to a biennial bearing behaviour of alternating crop loads in a branch or tree. The hormonal stimuli that elicit flowering is typically referred to as the floral induction (FI) phase in bud meristem development. To determine the metabolic pathways activated in FI, young trees of the cultivar 'Ruby Matilda' were subjected to zonal crop load treatments imposed to two leaders of bi-axis trees in the 2020/2021 season. Buds were collected over the expected FI phase, which is within 60 DAFB. Metabolomics profiling was undertaken to determine the differentially expressed pathways and key signalling molecules associated with FI in the leader and at tree level. Pronounced metabolic differences were observed in trees and leaders with high return bloom with significant increases in compounds belonging to the cytokinin, abscisic acid (ABA), phenylpropanoid and flavanol chemical classes. The presence of cytokinins, namely adenosine, inosine and related derivatives, as well as ABA phytohormones, provides further insight into the chemical intervention opportunities for future crop load management strategies via plant growth regulators.

Exposure to digital vape marketing among young people in Aotearoa New Zealand.

AIMS: Little is known about the exposure of young people in Aotearoa New Zealand to marketing of vape products on social media. This study investigated vaping behaviour and the extent of vape marketing exposure and engagement that young people (14-20 years) report on social media and examined differences across socio-demographic groups. METHODS: An online survey was conducted with 3,698 participants aged between 14-20 years (M=17.1; SD=1.8). A range of genders (55.7% females, 38.3% males and 6% another gender), ethnicities (25.6% Maori, 46.7% Pakeha or NZ European, 6.5% Pasifika and 21.2% another ethnicity) and social classes took part. RESULTS: Half (50.8%; n=1,110) of the respondents (N=2,185) reported that they had vaped at least once; vaping history was positively related to exposure to and engagement with digital vape marketing. Half (50.3%; n=1,119) of the respondents (N=2,224) reported seeing vape marketing on at least one social media platform. Binary logistic regressions showed that younger respondents were more likely to report seeing vape marketing than older respondents, and Maori and Pasifika more likely than other ethnicities. Over a quarter (26%; n=563) of respondents (N=2,148) reported engaging with vape marketing online, with Maori and Pasifika respondents more likely to engage than other ethnicity groups, and similarly for respondents of lower compared to higher socio-economic status. No interaction effects were found. CONCLUSIONS: Many young people, including a subset under the legal age for purchase, reported seeing vape product marketing on social media platforms. Patterns of exposure to vape product marketing on social media mirror the inequitable marketing exposure of harmful commodities in physical environments. Improved transparency and regulation of social media marketing is required.

Influence of reef isostasy, dynamic topography, and glacial isostatic adjustment on sea-level records in Northeastern Australia.

Understanding sea level during the peak of the Last Interglacial (125,000 yrs ago) is important for assessing future ice-sheet dynamics in response to climate change. The coasts and continental shelves of northeastern Australia (Queensland) preserve an extensive Last Interglacial record in the facies of coastal strandplains onland and fossil reefs offshore. However, there is a discrepancy, amounting to tens of meters, in the elevation of sea-level indicators between offshore and onshore sites. Here, we assess the influence of geophysical processes that may have changed the elevation of these sea-level indicators. We modeled sea-level change due to dynamic topography, glacial isostatic adjustment, and isostatic adjustment due to coral reef loading. We find that these processes caused relative sea-level changes on the order of, respectively, 10 m, 5 m, and 0.3 m. Of these geophysical processes, the dynamic topography predictions most closely match the tilting observed between onshore and offshore sea-level markers.

Programmatic alcohol advertising, social media and public health: Algorithms, automated challenges to regulation, and the failure of public oversight.

Ongoing partnerships between transnational alcohol corporations and global social media platforms have transformed alcohol advertising in ways that undermine existing marketing regulations and threaten all forms of public oversight. In this paper I analyse this historic shift, arguing that new forms of programmatic alcohol advertising have emerged out of increasingly intensive processes of datafication on social media. Programmatic advertising is distinguished by increasing automation, turning advertising into a process of continually experimenting on the public to optimise advertising's "outputs", which now include conversions (sales). This experimental process relies on the delegation of agency to profit maximising, algorithmic machines that make alcohol advertising decisions with public health consequences. I contend that public health must now grapple with "algorithmic sovereignty": how private, corporate machines make decisions that challenge regulation and public oversight. Drawing on a case study of Meta's programmatic systems, I argue programmatic alcohol advertising exacerbates existing problems for the regulatory control of alcohol advertising on social media, while introducing entirely novel concerns. Machines make decisions in ways that are efficacious yet obscure, even to those who design and own them and to those who use them for marketing purposes. They allow for the maximisation of profit while obfuscating public oversight of the health impacts of alcohol advertising, and they make decisions in ways that are both predictive and pre-emptive, continuously nudging social media users towards consumption through altering, in real-time, the personalised content they encounter. This leaves established means for public oversight of alcohol marketing operating retrospectively while commodification of social life through alcohol advertising acts in the present to reshape the future. The machinic vision of programmatic alcohol advertising assesses social media users for their commercial utility, not as citizens with health needs, and may be producing evolving negative health outcomes that will only ever be understood in retrospect once their deleterious impact has become evident. Steps towards more effective public health interventions must begin with clearer recognition of these fundamental changes in advertising.

Metabolic Pathways for Observed Impacts of Crop Load on Floral Induction in Apple.

The triggers of biennial bearing are thought to coincide with embryonic development in apple and occurs within the first 70 days after full bloom (DAFB). Strong evidence suggests hormonal signals are perceived by vegetative apple spur buds to induce flowering. The hormonal response is typically referred to as the floral induction (FI) phase in bud meristem development. To determine the metabolic pathways activated in FI, young trees of the biennial bearing cultivar 'Nicoter' and the less susceptible cultivar 'Rosy Glow' were forced into an alternate cropping cycle over five years and an inverse relationship of crop load and return bloom was established. Buds were collected over a four-week duration within 70 DAFB from trees that had maintained a four-year biennial bearing cycle. Metabolomics profiling was undertaken to determine the differentially expressed pathways and key signalling molecules associated with biennial bearing. Marked metabolic differences were observed in trees with high and low crop load treatments. Significant effects were detected in members of the phenylpropanoid pathway comprising hydroxycinnamates, salicylates, salicylic acid biosynthetic pathway intermediates and flavanols. This study identifies plant hormones associated with FI in apples using functional metabolomics analysis.

The Effects of Canopy Height and Bud Light Exposure on the Early Stages of Flower Development in Prunus persica (L.) Batsch.

The aims of this study were to investigate the sunlight requirements during floral initiation and differentiation for the development of flower buds in 'Autumn Bright' nectarine and to explore its source-sink relationship. In early January 2019 (111 days after full bloom), prior to floral initiation and differentiation, 12 new shoots were tagged on 14 trees, with four shoots in each of the low (0-1.2 m), middle (1.2-2.0 m), and high (>2.0 m) canopy heights. Three treatments (bud shading; leaf pluck; bud shading and leaf pluck) were applied to three shoots in each canopy height on the fourth and eighth bud, in addition to a fourth control shoot. Light penetration was measured at the different canopy heights. Buds were assessed in Spring for floral transition, number of floral buds per node, and fruit set. The treatments at the node level had no effect on floral initiation, indicating that sink strength was not promoted by additional light. Light penetration decreased with decreasing canopy height and corresponded with lower floral buds in the low zone. Fruit set was uninfluenced by all treatments. The results of this study emphasised the importance of the availability of photosynthetic assimilates for floral initiation in peach and nectarine trees. Balanced crop load management and summer pruning to enhance canopy sunlight distribution would increase the availability of nutrients for improved floral transition in this cultivar.

Effect of Shading on Red Colour and Fruit Quality in Blush Pears "ANP-0118" and "ANP-0131".

Some cultivars of Pyrus communis develop mature fruit with a distinctive red blush. Investigating the patterns of pear colour development in response to sunlight has implications for orchard management of these pears. The objectives of these experiments are to study the seasonal patterns of colour development and investigate the influence of shade and sunlight exposure on the red colour and harvest quality of blush pears "ANP-0118" and "ANP-0131". Several long, medium and short shading treatments were applied at different stages of fruit development from 28 ("ANP-0131") and 29 ("ANP-0118") days after full bloom (DAFB) until harvests at 119 DAFB ("ANP-0118") and 175 DAFB ("ANP-0131"). Fruits were measured every three weeks for colour parameters (a*, hue angle, chroma) and at harvest for quality parameters (fresh weight, visual assessments of percentage blush coverage and blush intensity, flesh firmness and soluble solids concentration). In the unshaded control, red colour increased during the growing season (increase in a* value and decrease in hue angle), as well as increasing in chroma value. Periods of shading during the season negatively affected red colour in both cultivars, as evidenced by significant decreases in a* value and increases in hue angle. Shaded fruits that were subsequently re-exposed to sunlight reacted with a dynamic increase in a* value and decrease in hue angle. Fruit shaded for the length of the experiment or prior to harvest had significantly lower a* values than the control at harvest. Visual assessment at harvest of percentage blush coverage and blush intensity were significantly affected by shading in both cultivars. Shading treatments applied early in the experiment had a negative effect on the fresh fruit weight of "ANP-0118".

Shelf sand supply determined by glacial-age sea-level modes, submerged coastlines and wave climate.

Submerged paloeshorelines preserved on the continental shelf indicate the depths of the most frequent (modal) low sea-levels within the glacial stages of the Late Quaternary. Here we have determined the south-east Australian shelf configuration when sea level was 40 m and 60 m below present-day sea-level (depths of the most persistent paleoshorelines within the last 120 ka), and we resolve the wave climate variations influencing the sediment transport pathways over this period. We present evidence demonstrating that the combination of shelf morphological evolution, changes in sea-level and variations in wave climate is responsible for latitudinal changes in sediment transport and deposition during the interglacial states. The paleoshoreline and shelf evolution is key to understanding the distribution of present-day shelf sand deposits and the contemporary sand budget response to future wave climate changes.

Active and Passive Electro-Optical Sensors for Health Assessment in Food Crops.

In agriculture, early detection of plant stresses is advantageous in preventing crop yield losses. Remote sensors are increasingly being utilized for crop health monitoring, offering non-destructive, spatialized detection and the quantification of plant diseases at various levels of measurement. Advances in sensor technologies have promoted the development of novel techniques for precision agriculture. As in situ techniques are surpassed by multispectral imaging, refinement of hyperspectral imaging and the promising emergence of light detection and ranging (LIDAR), remote sensing will define the future of biotic and abiotic plant stress detection, crop yield estimation and product quality. The added value of LIDAR-based systems stems from their greater flexibility in capturing data, high rate of data delivery and suitability for a high level of automation while overcoming the shortcomings of passive systems limited by atmospheric conditions, changes in light, viewing angle and canopy structure. In particular, a multi-sensor systems approach and associated data fusion techniques (i.e., blending LIDAR with existing electro-optical sensors) offer increased accuracy in plant disease detection by focusing on traditional optimal estimation and the adoption of artificial intelligence techniques for spatially and temporally distributed big data. When applied across different platforms (handheld, ground-based, airborne, ground/aerial robotic vehicles or satellites), these electro-optical sensors offer new avenues to predict and react to plant stress and disease. This review examines the key sensor characteristics, platform integration options and data analysis techniques recently proposed in the field of precision agriculture and highlights the key challenges and benefits of each concept towards informing future research in this very important and rapidly growing field.

A robust impact assessment that informs actionable climate change adaptation: future sunburn browning risk in apple.

Climate change impact assessments are predominantly undertaken for the purpose of informing future adaptation decisions. Often, the complexity of the methodology hinders the actionable outcomes. The approach used here illustrates the importance of considering uncertainty in future climate projections, at the same time providing robust and simple to interpret information for decision-makers. By quantifying current and future exposure of Royal Gala apple to damaging temperature extremes across ten important pome fruit-growing locations in Australia, differences in impact to ripening fruit are highlighted, with, by the end of the twenty-first century, some locations maintaining no sunburn browning risk, while others potentially experiencing the risk for the majority of the January ripening period. Installation of over-tree netting can reduce the impact of sunburn browning. The benefits from employing this management option varied across the ten study locations. The two approaches explored to assist decision-makers assess this information (a) using sunburn browning risk analogues and (b) through identifying hypothetical sunburn browning risk thresholds, resulted in varying recommendations for introducing over-tree netting. These recommendations were location and future time period dependent with some sites showing no benefit for sunburn protection from nets even by the end of the twenty-first century and others already deriving benefits from employing this adaptation option. Potential best and worst cases of sunburn browning risk and its potential reduction through introduction of over-tree nets were explored. The range of results presented highlights the importance of addressing uncertainty in climate projections that result from different global climate models and possible future emission pathways.

A conserved OmpA-like protein in Legionella pneumophila required for efficient intracellular replication.

The OmpA-like protein domain has been associated with peptidoglycan-binding proteins, and is often found in virulence factors of bacterial pathogens. The intracellular pathogen Legionella pneumophila encodes for six proteins that contain the OmpA-like domain, among them the highly conserved uncharacterized protein we named CmpA. Here we set out to characterize the CmpA protein and determine its contribution to intracellular survival of L. pneumophila Secondary structure analysis suggests that CmpA is an inner membrane protein with a peptidoglycan-binding domain at the C-teminus. A cmpA mutant was able to replicate normally in broth, but failed to compete with an isogenic wild-type strain in an intracellular growth competition assay. The cmpA mutant also displayed significant intracellular growth defects in both the protozoan host Acanthamoeba castellanii and in primary bone marrow-derived macrophages, where uptake into the cells was also impaired. The cmpA phenotypes were completely restored upon expression of CmpA in trans The data presented here establish CmpA as a novel virulence factor of L. pneumophila that is required for efficient intracellular replication in both mammalian and protozoan hosts.

Social networking and young adults' drinking practices: innovative qualitative methods for health behavior research.

OBJECTIVES: Understandings of health behaviors can be enriched by using innovative qualitative research designs. We illustrate this with a project that used multiple qualitative methods to explore the confluence of young adults' drinking behaviors and social networking practices in Aotearoa, New Zealand. METHOD: Participants were 18-25 year old males and females from diverse ethnic, class, and occupational backgrounds. In Stage 1, 34 friendship focus group discussions were video-recorded with 141 young adults who talked about their drinking and social networking practices. In Stage 2, 23 individual interviews were conducted using screen-capture software and video to record participants showing and discussing their Facebook pages. In Stage 3, a database of Web-based material regarding drinking and alcohol was developed and analyzed. RESULTS: In friendship group data, young adults co-constructed accounts of drinking practices and networking about drinking via Facebook as intensely social and pleasurable. However, this pleasure was less prominent in individual interviews, where there was greater explication of unpleasant or problematic experiences and practices. The pleasure derived from drinking and social networking practices was also differentiated by ethnicity, gender, and social class. Juxtaposing the Web-based data with participants' talk about their drinking and social media use showed the deep penetration of online alcohol marketing into young people's social worlds. CONCLUSIONS: Multiple qualitative methods, generating multimodal datasets, allowed valuable nuanced insights into young adults' drinking practices and social networking behaviors. This knowledge can usefully inform health policy, health promotion strategies, and targeted health interventions.

Deconvoluting effects of vine and soil properties on grape berry composition.

BACKGROUND: Grape berry composition is influenced by several factors including grapevine and soil properties and their interactions. Understanding how these factors interact to determine berry composition is integral to producing berries with desired composition. Here we used extensive spatio-temporal data to identify significant vine and soil features that influence Shiraz berry composition. RESULTS: The concentrations of berry flavonoids (anthocyanins, tannin and total phenolics), total soluble solids and pH were typically negatively associated with canopy, crop and berry size factors whereas titratable acidity was positively associated. The strengths of the associations, however, were generally greater with the crop and berry size factors than with the canopy size factor. The analyses also resolved separate influences of berry and crop size on berry composition. Soil properties had significant influences on berry composition; however, when influences of soil factors on vine-attributes were accounted for, the apparent effects of soil factors on berry composition were largely non-existent. CONCLUSION: At each site, variations in berry composition were more strongly associated with crop and berry size than with canopy size factors. Apparent influences of soil properties on berry composition are indirect, being mediated via their effects on vine attributes (canopy, crop and berry sizes).

Climate windows for Polynesian voyaging to New Zealand and Easter Island.

Debate about initial human migration across the immense area of East Polynesia has focused upon seafaring technology, both of navigation and canoe capabilities, while temporal variation in sailing conditions, notably through climate change, has received less attention. One model of Polynesian voyaging observes that as tradewind easterlies are currently dominant in the central Pacific, prehistoric colonization canoes voyaging eastward to and through central East Polynesia (CEP: Society, Tuamotu, Marquesas, Gambier, Southern Cook, and Austral Islands) and to Easter Island probably had a windward capacity. Similar arguments have been applied to voyaging from CEP to New Zealand against prevailing westerlies. An alternative view is that migration required reliable off-wind sailing routes. We investigate the marine climate and potential voyaging routes during the Medieval Climate Anomaly (MCA), A.D. 800-1300, when the initial colonization of CEP and New Zealand occurred. Paleoclimate data assimilation is used to reconstruct Pacific sea level pressure and wind field patterns at bidecadal resolution during the MCA. We argue here that changing wind field patterns associated with the MCA provided conditions in which voyaging to and from the most isolated East Polynesian islands, New Zealand, and Easter Island was readily possible by off-wind sailing. The intensification and poleward expansion of the Pacific subtropical anticyclone culminating in A.D. 1140-1260 opened an anomalous climate window for off-wind sailing routes to New Zealand from the Southern Austral Islands, the Southern Cook Islands, and Tonga/Fiji Islands.

'See it doesn't look pretty does it?' Young adults' airbrushed drinking practices on Facebook.

A range of negative health outcomes are associated with young adults' drinking practices. One key arena where images of, and interaction about, drinking practices occurs is social networking sites, particularly Facebook. This study investigated the ways in which young adults' talked about and understood their uses of Facebook within their drinking practices. Face-to-face, semi-structured interviews were conducted with seven New Zealand young adults as they displayed, navigated and talked about their Facebook pages and drinking behaviours. Our social constructionist thematic analysis identified three major themes, namely 'friendship group belonging', 'balanced self-display' and 'absences in positive photos'. Drinking photos reinforced friendship group relationships but time and effort was required to limit drunken photo displays to maintain an overall attractive online identity. Positive photos prompted discussion of negative drinking events which were not explicitly represented. Together these understandings of drinking photos function to delimit socially appropriate online drinking displays, effectively 'airbrushing' these visual depictions of young adults' drinking as always pleasurable and without negative consequences. We consider the implications of these findings for ways alcohol health initiatives may intervene to reframe 'airbrushed' drinking representations on Facebook and provoke a deeper awareness among young people of drinking practices and their online displays.

Challenges in predicting climate change impacts on pome fruit phenology.

Climate projection data were applied to two commonly used pome fruit flowering models to investigate potential differences in predicted full bloom timing. The two methods, fixed thermal time and sequential chill-growth, produced different results for seven apple and pear varieties at two Australian locations. The fixed thermal time model predicted incremental advancement of full bloom, while results were mixed from the sequential chill-growth model. To further investigate how the sequential chill-growth model reacts under climate perturbed conditions, four simulations were created to represent a wider range of species physiological requirements. These were applied to five Australian locations covering varied climates. Lengthening of the chill period and contraction of the growth period was common to most results. The relative dominance of the chill or growth component tended to predict whether full bloom advanced, remained similar or was delayed with climate warming. The simplistic structure of the fixed thermal time model and the exclusion of winter chill conditions in this method indicate it is unlikely to be suitable for projection analyses. The sequential chill-growth model includes greater complexity; however, reservations in using this model for impact analyses remain. The results demonstrate that appropriate representation of physiological processes is essential to adequately predict changes to full bloom under climate perturbed conditions with greater model development needed.

"Everyone can loosen up and get a bit of a buzz on": young adults, alcohol and friendship practices.

In countries with liberalised alcohol policies, alcohol harm reduction strategies predominantly focus on young adults' excessive drinking harms and risks. However, research shows such risks are largely irrelevant for young adults, who emphasise the sociability, release, pleasure and fun of drinking. Friendship is a central part of their lives and an integral part of their drinking experiences. This study aimed to explore everyday friendship practices, drinking, and pleasure in young people's routine and shared social lives. Twelve friendship discussion groups were conducted in urban and non-urban New Zealand, with 26 women and 25 men aged 18-25 years. Our Foucauldian discursive analysis enabled us to identify how the young adults drew on drinking as 'friendship fun' and 'friends with a buzz' discourses to construct drinking as a pleasurable and socially embodied friendship practice. Yet the young adults also drew on 'good always outweighs bad experiences' and friendship 'caring and protection' discourses to smooth over disruptive negative drinking experiences. Together these discourses function to justify young adults' drinking as friendship pleasure, minimising alcohol harms, and setting up powerful resistances to individualised risk-based alcohol-harm reduction campaigns. These findings are discussed in terms of new insights and implications for alcohol harm reduction strategies that target young adults.

Impact of future warming on winter chilling in Australia.

Increases in temperature as a result of anthropogenically generated greenhouse gas (GHG) emissions are likely to impact key aspects of horticultural production. The potential effect of higher temperatures on fruit and nut trees' ability to break winter dormancy, which requires exposure to winter chilling temperatures, was considered. Three chill models (the 0-7.2°C, Modified Utah, and Dynamic models) were used to investigate changes in chill accumulation at 13 sites across Australia according to localised temperature change related to 1, 2 and 3°C increases in global average temperatures. This methodology avoids reliance on outcomes of future GHG emission pathways, which vary and are likely to change. Regional impacts and rates of decline in chilling differ among the chill models, with the 0-7.2°C model indicating the greatest reduction and the Dynamic model the slowest rate of decline. Elevated and high latitude eastern Australian sites were the least affected while the three more maritime, less elevated Western Australian locations were shown to bear the greatest impact from future warming.

Evaluation of recent trends in Australian pome fruit spring phenology.

Temporal and temperature driven analyses were conducted for eight spring phenology datasets from three Australian pome fruit growing regions ranging from 24 to 43 years in length. This, the first such analysis for Australia, indicated significant temporal change in phenophase timing for only one of the datasets. To determine relationships to temperature, a sequential chill and growth method as well as mean springtime temperatures were used to estimate phenophase timing. Expected advancement of phenophase ranged from 4.1 to 7.7 days per degree Celsius increase in temperature. The sequential chill and growth approach proved superior, with coefficients of determination between 0.49 and 0.85, indicating the inclusion of chill conditions are important for spring phenology modelling. Compared to similar phenological research in the Northern Hemisphere, the changes in response variables were often shallower in Australia, although significance of observed hemispheric differences were not found.

Nourishment practices on Australian sandy beaches: a review.

It is predicted that the coastal zone will be among the environments worst affected by projected climate change. Projected losses in beach area will negatively impact on coastal infrastructure and continued recreational use of beaches. Beach nourishment practices such as artificial nourishment, replenishment and scraping are increasingly used to combat beach erosion but the extent and scale of projects is poorly documented in large areas of the world. Through a survey of beach managers of Local Government Areas and a comprehensive search of peer reviewed and grey literature, we assessed the extent of nourishment practices in Australia. The study identified 130 beaches in Australia that were subject to nourishment practices between 2001 and 2011. Compared to projects elsewhere, most Australian projects were small in scale but frequent. Exceptions were nine bypass projects which utilised large volumes of sediment. Most artificial nourishment, replenishment and beach scraping occurred in highly urbanised areas and were most frequently initiated in spring during periods favourable to accretion and outside of the summer season of peak beach use. Projects were generally a response to extreme weather events, and utilised sand from the same coastal compartment as the site of erosion. Management was planned on a regional scale by Local Government Authorities, with little monitoring of efficacy or biological impact. As rising sea levels and growing coastal populations continue to put pressure on beaches a more integrated approach to management is required, that documents the extent of projects in a central repository, and mandates physical and biological monitoring to help ensure the engineering is sustainable and effective at meeting goals.