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1.
J Bacteriol ; 185(15): 4609-14, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12867472

ABSTRACT

We have carried out a mutational scan of the upstream region of the bacteriophage P2 FETUD late operon promoter, P(F), which spans an element of hyphenated dyad symmetry that is conserved among all six of the P2 and P4 late promoters. All mutants were assayed for activation by P4 delta in vivo, by using a lacZ reporter plasmid, and a subset of mutants was assayed in vitro for delta binding. The results confirm the critical role of the three complementary nucleotides in each half site of the upstream element for transcription factor binding and for activation of transcription. A trinucleotide DNA recognition site is consistent with a model in which these transcription factors bind via a zinc finger motif. The mutational scan also led to identification of the -35 region of the promoter. Introduction of a sigma(70) -35 consensus sequence resulted in increased constitutive expression, which could be further stimulated by delta. These results indicate that activator binding to the upstream region of P2 late promoters compensates in part for poor sigma(70) contacts and helps to recruit RNA polymerase holoenzyme.


Subject(s)
Bacteriophage P2/genetics , Gene Expression Regulation, Viral , Promoter Regions, Genetic/physiology , Transcription, Genetic , Base Sequence , Molecular Sequence Data , Mutation , Operon , Promoter Regions, Genetic/genetics , Viral Proteins/genetics , Viral Proteins/metabolism
2.
J Bacteriol ; 184(23): 6522-31, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12426340

ABSTRACT

The major structural components of the P2 contractile tail are encoded in the FETUD tail gene operon. The sequences of genes F(I) and F(II), encoding the major tail sheath and tail tube proteins, have been reported previously (L. M. Temple, S. L. Forsburg, R. Calendar, and G. E. Christie, Virology 181:353-358, 1991). Sequence analysis of the remainder of this operon and the locations of amber mutations Eam30, Tam5, Tam64, Tam215, Uam25, Uam77, Uam92, and Dam6 and missense mutation Ets55 identified the coding regions for genes E, T, U, and D, completing the sequence determination of the P2 genome. Inspection of the DNA sequence revealed a new open reading frame overlapping the end of the essential tail gene E. Lack of an apparent translation initiation site and identification of a putative sequence for a programmed translational frameshift within the E gene suggested that this new reading frame (E') might be translated as an extension of gene E, following a -1 translational frameshift. Complementation analysis demonstrated that E' was essential for P2 lytic growth. Analysis of fusion polypeptides verified that this reading frame was translated as a -1 frameshift extension of gpE, with a frequency of approximately 10%. The arrangement of these two genes within the tail gene cluster of phage P2 and their coupling via a translational frameshift appears to be conserved among P2-related phages. This arrangement shows a striking parallel to the organization in the tail gene cluster of phage lambda, despite a lack of amino acid sequence similarity between the tail gene products of these phage families.


Subject(s)
Bacteriophage P2/genetics , Frameshifting, Ribosomal , Gene Expression Regulation, Viral , Operon , Viral Tail Proteins/genetics , Amino Acid Sequence , Base Sequence , DNA, Viral/analysis , Molecular Sequence Data , Sequence Analysis, DNA , Viral Tail Proteins/metabolism
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