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1.
Appl Microbiol ; 25(3): 325-6, 1973 Mar.
Article in English | MEDLINE | ID: mdl-4349184

ABSTRACT

An antibody-free human serum fraction has been prepared by ammonium sulfate precipitation. This fraction can be substituted for whole serum in the production of optimal yields of interferon by induced human leukocytes.


Subject(s)
Immune Sera , Interferons/biosynthesis , Leukocytes/metabolism , Ammonium Sulfate , Amnion , Chemical Precipitation , Chromatography, Gel , Culture Techniques , Electrophoresis, Polyacrylamide Gel , Humans , Immunodiffusion , Immunoglobulins/isolation & purification , Interferons/analysis , Parainfluenza Virus 1, Human , Poliovirus/growth & development
3.
Infect Immun ; 6(5): 886-8, 1972 Nov.
Article in English | MEDLINE | ID: mdl-4564788

ABSTRACT

An interferon-inducing RNA, SK&F 42319, obtained from a lysate of phage-infected Escherichia coli, protected mice against Vibrio cholerae infection. This nonspecific protection was not attributable to endotoxin contamination.


Subject(s)
Bacteriophages , Escherichia coli/metabolism , RNA, Bacterial/pharmacology , Vibrio/immunology , Animals , Endotoxins/pharmacology , Interferon Inducers/pharmacology , Liver/enzymology , Male , Mice , Tryptophan Oxygenase/metabolism , Vibrio Infections/immunology
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