Complex interactions among temperature, microplastics and cyanobacteria may facilitate cyanobacteria proliferation and microplastic deposition.

Cyanobacterial blooms are a global concern prone to causing environmental and economic damages and are tightly linked to anthropogenic nutrient inputs. Likewise, microplastic pollution has also become globally ubiquitous inevitably co-occurring with blooms. However, little is known on how microplastics influence cyanobacterial physiologically and how potential physiological changes can affect their buoyancy, ultimately impacting their fate, and transport, including deposition during bloom events. Interactions of environmental relevant concentrations of high-density polyethylene microplastics (MPs) (0-0.4 mg/mL) and temperatures (2.5-32.5 °C) were evaluated to assess the effects of MPs on interactions of cyanobacteria Anabaena variabilis's growth, total organic carbon concentrations, extracellular polymeric substances (EPS) production, and MP deposition. Microplastics both stimulated and inhibited A. variabilis growth depending on the concentration. Lower MPs concentrations (0.1-0.2 mg/L) increased A. variabilis growth while higher MP concentrations (>0.3 mg/mL) impeded it across all temperatures studied. Carbon sources leached from MPs may have been a contributing factor to the increased growth at lower MPs concentration, while higher MPs concentration potentially shaded A. variabilis inhibiting its growth. Shading may have induced stress which corresponded with an observed increase in EPS production by A. variabilis when exposed to MP. Extracellular polymeric substances generation activated under adverse circumstances (MPs 0.4 mg/mL) enhanced MP deposition. Overall, our findings indicate that MPs play an important role in cyanobacterial blooms, and that these blooms may enhance MPs deposition.

Identification of Optimal Calcium and Temperature Conditions for Quagga Mussel Filtration Rates as a Potential Predictor of Invasion.

Quagga mussels (Dreissena rostriformis burgensis) are a highly invasive aquatic species to North America, capable of filtering large volumes of water and causing severe ecological and economic impacts. Their range has been expanding since they first invaded the Great Lakes in the 1980s. To predict their spread, it is crucial to understand environmental parameters, which facilitate their range expansion. Two factors likely to influence their distribution include calcium and temperature, because the former is vital for shell development and the latter for metabolic activity. When these factors are optimal for mussels' fitness, the filtration rate has the potential to be maximized if other environmental conditions are also favorable, thus enabling mussels to exploit their growth potential. Deviations from optimal conditions likely result in filtration-rate decline. We identify calcium concentrations and temperatures that maximize the mussel filtration rate for 2 phytoplankton species: Ankistrodesmus facaltus, a common food source for quagga mussels, and a less palatable Microcystis icthyoblabe. In laboratory experiments, filtration rates were measured through cell counts after 24 h of filtration when exposed to a range of temperatures between 2 and 30 °C, and calcium concentrations between 0 and 180 mg/L. Response surface methodology was used to identify a maximum filtration rate, which occurred at 22 mL/mg/h at 137 mg/L of calcium carbonate and 26 °C when fed Ankistrodesmus. To establish a quagga mussel population in a new water source, optimum conditions are required; thus, this information can be used to rank the relative susceptibility of water bodies to invasion by quagga mussels. Environ Toxicol Chem 2020;39:410-418. © 2019 SETAC.