Passive fungal spore release from fruit and vegetable solid waste.

Food substrates in municipal solid wastes processing facilities and open dumpsites are a source for the release of fungal spores into air and can cause potential health and climate effects. Experiments were conducted in a laboratory scale flux chamber to measure the fungal growth and spore release from representative exposed cut fruit and vegetable substrates. The aerosolised spores were measured using an optical particle sizer. The results were compared to experiments conducted previously with a test species (Penicillium chrysogenum) on a synthetic media (czapek yeast extract agar). Significantly higher surface spore densities were observed for the fungi on the food substrates as compared to that on the synthetic media. The spore flux was high initially and then decreased on continued exposure to air. The spore emission flux normalised to the surface spore densities indicated that the emission from the food substrates was lower than the emissions from the synthetic media. A mathematical model was applied to the experimental data and the observed flux trends were explained in terms of the model parameters. A simple application of the data and the model to release from a municipal solid waste dumpsite was shown.

Adsorption of Triclosan on Nylon 66 Membrane: Preconcentration and Ultrasensitive Colorimetric Detection.

In this work, adsorption of triclosan (TCS) on nylon 66 membrane is explored to develop a preconcentration and sensing platform. Nylon 66 membrane exhibits superior sorption ability even for trace amounts of TCS (10 µg/L). Investigating the surface adsorption chemistry by XPS analysis revealed the formation of a hydrogen bond between the hydroxyl group of TCS and the amide group of nylon 66. In the absence of TCS, the amphiprotic water molecule forms a multilayer OH group on the membrane surface. However, TCS showed preferential adsorption on the membrane-replacing water molecule due to its higher hydrophobic partition coefficient. We validated the effective preconcentration of TCS on the membrane using LC-MS analysis. Performing colorimetry directly on the TCS-enriched membrane surface showed a visible color change for concentrations as low as 10 µg/L. The relative blue intensity was found to vary linearly over a concentration range of 10-100 µg/L, and we achieved a detection limit of 7 µg/L for a 5 mL sample. This method utilizes easy-to-use resources which drastically reduce the cost and complexity of analysis.

Adsorptive colorimetric determination of chromium(VI) ions at ultratrace levels using amine functionalized mesoporous silica.

There is an urgent need for a rapid, affordable and sensitive analytical method for periodic monitoring of heavy metals in water bodies. Herein, we report for the first time a versatile method for ultratrace level metal detection based on colorimetric sensing. The method integrates preconcentration using a nanomaterial with a colorimetric assay performed directly on the metal-enriched nanomaterial surface. This method circumvents the need for tedious sample pre-processing steps and the complex development of colorimetric probes, thereby reducing the complexity of the analytical procedure. The efficacy of the proposed method was demonstrated for chromium(VI) ions detection in water samples. Amine functionalized mesoporous silica (AMS) obtained from a one-pot synthesis was utilized as a pre-concentration material. The structural and chemical analysis of AMS was conducted to confirm its physico-chemical properties. The pre-concentration conditions were optimized to maximise the colorimetric signal. AMS exhibited a discernible colour change from white to purple (visible to the naked eye) for trace Cr(VI) ions concentration as low as 0.5 µg L-1. This method shows high selectivity for Cr(VI) ions with no colorimetric signal from other metal ions. We believe our method of analysis has a high scope for de-centralized monitoring of organic/inorganic pollutants in resource-constrained settings.

Sensitive and selective determination of triclosan using visual spectroscopy.

Triclosan is a commonly used biocide effective against bacterial and fungal infections. However, its overuse in pharmaceutical and personal care products has resulted in its abundance in the natural environment. The detection of triclosan by visual spectroscopy can be carried out using the azo-coupling reaction of diazonium complexes. However, the reaction is also common to other phenolic compounds and aromatic amines, posing significant challenge. In this work, we investigate the azo-coupling reaction of triclosan and several commonly occurring analogous compounds to develop an improved spectroscopic method for the selective determination of triclosan without interference. We find that the azo-coupling reaction between the diazotized derivative and the phenolic compounds is highly dependent on the pH of the reaction media. At pH 7.2, the absorbance of the azo dye product of triclosan shows a peak at 452 nm which has minimal interference from other phenolic azo-dye products with the exception of naphthol. Naphthol shows an interference corresponding to 58% of the analytical signal of equimolar triclosan concentration. To overcome this, we develop an analytical model for the simultaneous determination of triclosan and naphthol from mixed solutions of the compounds. A linear calibration plot from 1.7 to 34 µM was obtained for both triclosan and naphthol with limit-of-detection (LOD) of 0.62 µM and 1.03 µM respectively. The developed protocol was tested for the analysis of water samples collected from various environmental sources spiked with different concentrations of triclosan and naphthol. The samples were enriched by solid-phase-extraction which allowed a 50-fold enhancement in detection of triclosan. The average relative recovery of triclosan in real samples was found to be 98.6% .

Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications.

Microfluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein, we report for the first time, a facile method to fabricate µPADs using a commonly available laser printer which drastically reduces the cost and complexity of fabrication. Toner ink is used to pattern the µPADs by printing, without modifying any factory configuration of the laser printer. Hydrophobic barriers are created by heating the patterned paper which melts the toner ink, facilitating its wicking into the cross-section of the substrate. Further, we demonstrate the utilization of the fabricated device by performing two assays. The proposed technique provides a versatile platform for rapid prototyping of µPADs with significant prospect in both developed and resource constrained region.