[Metabolism of adenosine and thymidine in healthy females of different ages and females with mastopathies]. / Metabolizm adenozynu ta tymidynu v orhanizmi zdorovykh ta khvoryk na mastopatiiu zhinok riznoho viku.

The activity of thymidine kinase, thymidine phosphorylase, adenosine deaminase and 5'-nucleotidase of AMP was studied in tissues of 39 healthy females, as well as blood serum and lymphocytes of 60 healthy females, as well as in 50 patients with fibrocavernous mastopathy aged as 23-70. Comparative determination of adenosine metabolism enzymes activity in lymphocytes was carried out simultaneously with studying some immunological indexes in the organism of the same-aged healthy females and ones with mastopathy. It was revealed that age-related changes in the activity of thymidine kinase in blood serum reflected the analogous changes in enzyme activity in tissues of the healthy women. A direct correlation was established between thymidine kinase activity and age both in the healthy females and those with mastopathy. A significant decrease in activity of thymidine phosphorylase was demonstrated in blood serum of the patients with mastopathy in the age 46-60. Determined 4-fold increase in the activity of adenosine deaminase in serum was accompanied by decreased enzyme activity in lymphocytes and decreased Lymphocyte Blast Transformation Index in the same age range. Changes of immunological status are more expressed in T-system of immunity. The revealed metabolic changes in DNA-precursors metabolism in the patients with mastopathy aged as 46-60 might be one of the reasons of increased risk of oncological disease in this age group.

[The dynamics of the enzymatic activity of DNA metabolism during the treatment of stomach cancer patients]. / Dinamika aktivnosti fermentov metabolizma DNK v protsesse lecheniia bol'nykh bol'nykh rakom zheludka.

The activity of thymidine kinase was found to be distinctly elevated while the activity of thymidine phosphorylase was markedly reduced in the blood serum of patients with gastric cancer. The dynamics of enzymatic activity depended on the form of surgical intervention. In patients undergoing chemotherapy positive effects were observed in those with a high initial activity of thymidine phosphorylase. This enzyme test is recommended for determination of treatment efficiency and prognosis of the course of the disease.

[Activity of the enzymes of DNA metabolism in the blood of patients with breast cancer]. / Aktivnost' fermentov metabolizma DNK v syvorotke krovi bol'nykh rakom molochnoi zhelezy.

Age-related changes in the activity of thymidine- and adenosine-metabolizing enzymes were studied in healthy females and those with breast cancer aged 46-70 years. A significant increase in activity of thymidine kinase, adenosine deaminase and 5'-nucleotidase and a decrease in that of thymidine phosphorylase were registered in blood serum of breast cancer patients of all age brackets. Adenosine deaminase activity in blood serum and lymphocytes of breast cancer patients was found to significantly change after surgery. A direct correlation was established between pretreatment thymidine phosphorylase activity and histological type of tumor, on the one hand and results of chemotherapy, on the other. The applicability of enzyme level assay for evaluating response to pre- and postoperative medication was studied.

[The enzymatic activity of DNA metabolism of the blood in patients operated on in stomach cancer]. / Aktivnost' fermentov metabolizma DNK v krovi u bol'nykh, operirovannykh po povodu raka zheludka.

The study was concerned with comparison of the activity of thymidine kinase, thymidine phosphorylase, adenosine desaminase and AMP 5'-nucleotidase in serum of cancer patients and healthy subjects. A significant change in 5'-nucleotidase level was registered in patients over 60 years of age and in those of adenosine desaminase and thymidine phosphorylase in patients older than 70 years. A comparative evaluation of the activity of those enzymes showed an increase in thymidine kinase activity matched by a sharp drop in that of thymidine phosphorylase in patients over 45 years. A sharp increase in the activity of adenosine metabolizing enzymes in cancer serum was accompanied by its decrease in lymphocytes. Among surgical patients, significant changes in the activity were observed in radically operated cases only.

[Age-related characteristics of thymidine metabolism in healthy subjects and in patients with stomach ulcer and cancer]. / Vozrastnye osobennosti metabolizma timidina v organizme zdorovogo cheloveka, pri iazvennoi bolezni i rake zheludka.

Biosynthesis of pyrimidine nucleotides was intensified in proliferating tissues. Age-dependent alterations in activities of thymidine kinase and thymidine phosphorylase were found in healthy persons of 17-70 years old. Distinct increase in activity of thymidine kinase was observed in patients with gastric ulcer but the activity was decreased with ageing. Alterations in the ratio between anabolic and catabolic reactions involving thymidine were studied also in patients with gastric tumor depending on age.

[State of the sympathetic-adrenal system in newborn infants (clinico-biochemical comparisons)]. / O sostoianii simpatiko-adrenalovoi sistemy u novorozhdennykh (kliniko-biokhimicheskie sopostavleniia)

A study was made of urinary catecholamine excretion in 102 newborn boys on the 1st-8th day after birth. Depending on the state at birth neonates were divided into 2 groups: the 1st group included babies born in satisfactory condition (60), and the 2nd group - at the state of mild asphyxia. Catecholamines were determined on the 1st-2nd, 3rd-5th, and 6th-8th days after birth. It was shown that at the time of birth the noradrenaline level prevailed over the adrenaline level, but as soon as the 3rd-5th day the noradrenaline content displayed a relative reduction in both groups. Asphyxia showed practically no influence on the urinary adrenaline and noradrenaline excretion of the newborn boys; however, there were significant differences in the dynamics of dophamine excretion with the urine. The children born asphyxiated urinary excretion of dophamine showed a continuous accretion.