ABSTRACT
The samples of colon adenocarcinomas (CAC) and their lymph node metastases from 22 patients were studied. The expression of thymidylate synthase (TS), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), E-cadherin, beta-catenin, tenascin C (TN), and KAI-1/CD82 in primary CAC and their lymph node metastases was compared using an immunohistochemical method. The expression of TS, VEGF, EGFR, E-cadherin, TNC, and KAI-1 was statistically significant different in primary CAC and involved lymph nodes. Comparison of the expression of the markers in the pairs of primary CAC and metastasis revealed equal values for TNC and EFGR in 45.5 and 41% of cases, respectively; the number of coincidences in the expression of the other markers was insignificant. Determination of the expression of molecular biological markers in primary CAC and their lymph nodes may serve as a predictor of therapy response and have a prognostic value.
Subject(s)
Adenocarcinoma/mortality , Adenocarcinoma/pathology , Biomarkers, Tumor/biosynthesis , Colonic Neoplasms/metabolism , Colonic Neoplasms/pathology , Gene Expression Regulation, Neoplastic , Neoplasm Proteins/biosynthesis , Adenocarcinoma/therapy , Colonic Neoplasms/therapy , Female , Humans , Lymphatic Metastasis , Male , PrognosisABSTRACT
Colorectal adenocarcinoma and its lymph node metastases from 72 patients and 14 control samples were studied. Expression of adhesive molecules - E-cadherin and beta-catenin, antiadhesive molecule - tenascin C and tumor metastasis suppressor KAI-1 (CD82) were studied by immunohistochemistry. The expression of E-cadherin and beta-catenin, tenascin C and KAI-1 was significantly different in adenocarcinoma and control samples. The expression of E-cadherin, tenascin C and KAI-1 was diverse in primary tumor compared to lymph node metastases. The analysis of current data showed the association of E-cadherin expression with stage of disease and depth of invasion, such as the correlation of beta-catenin nuclear expression and tenascin C expression with depth of adenocarcinoma invasion. These data may be a useful indicator of disease progression.
Subject(s)
Adenocarcinoma , Biomarkers, Tumor/biosynthesis , Colorectal Neoplasms , Gene Expression Regulation, Neoplastic , Neoplasm Proteins/biosynthesis , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Aged , Colorectal Neoplasms/metabolism , Colorectal Neoplasms/pathology , Disease Progression , Female , Humans , Lymphatic Metastasis , Male , Middle AgedABSTRACT
Immunofluorescence assay has been widely used so far to diagnose glomerular and some skin diseases. The optimal antigen persistence is achieved using the frozen sections; however, their considerable shortcoming is the impossibility to long store preparations and to use a morphology archive. Our laboratory has modified a direct immunofluorescence study on paraffin-embedded renal and skin tissue sections, substantially increasing its accessibility.
Subject(s)
Fluorescent Antibody Technique, Direct/methods , Kidney/metabolism , Kidney/pathology , Skin/metabolism , Skin/pathology , HumansABSTRACT
The authors report a rare a case of gliosarcoma metastases in a 28-year-old male patient. The cauda equina roots were involved after brain tumor 16 months ago, which, on microscopic study, had a biphasic pattern and heterogeneous staining in the reaction with antibody to GFAP and vimentin; the tumor cells did not express EMA, EA, and desmin. Gliosarcoma was diagnosed, by taking into account morphological and immunohistochemical data. Tumor tissue of the cauda equine roots had the same immunophenotype as the brain tumor with a predominance of glial component, which permitted the source of metastases to be ascertained.
Subject(s)
Brain Neoplasms , Cauda Equina/pathology , Gliosarcoma , Peripheral Nervous System Neoplasms , Adult , Brain Neoplasms/metabolism , Brain Neoplasms/pathology , Brain Neoplasms/surgery , Cauda Equina/metabolism , Glial Fibrillary Acidic Protein/metabolism , Gliosarcoma/metabolism , Gliosarcoma/pathology , Humans , Male , Neoplasm Metastasis , Neoplasm Proteins/metabolism , Peripheral Nervous System Neoplasms/metabolism , Peripheral Nervous System Neoplasms/pathology , Peripheral Nervous System Neoplasms/secondary , Vimentin/metabolismABSTRACT
The expression of E-cadherin and beta-catenin in the eutopic and ectopic endometrium was studied in 40 patients with adenomyosis and 12 without this condition, by using an immunohistochemical test and enzyme immunoassay. There was increased expression of E-cadherin and beta-catenin in the eutopic and ectopic endometrium in adenomyosis. The cytoplasmic expression of beta-catenin was also revealed in the smooth muscle cells surrounding the foci of adenomyosis. The E-cadherin concentration measured by enzyme immunoassay was significantly higher in the endometrium and myometrium of patients with adenomyosis than in the controls. There was a higher expression of this protein with a longer duration of the disease. Thus, the formation of ectopic foci in adenomyosis may be argued to be unassociated with the decreased adhesion of epithelial cells. On the contrary, the authors documented the enhanced adhesion of epithelial cells in the ectopic foci, which was likely to be adaptive due to the altered microcirculation.
Subject(s)
Cadherins/metabolism , Endometriosis/metabolism , Endometrium/metabolism , Myometrium/metabolism , beta Catenin/metabolism , Cytoplasm/metabolism , Endometriosis/pathology , Endometrium/pathology , Female , Humans , Immunoenzyme Techniques , Immunohistochemistry , Muscle, Smooth/metabolism , Muscle, Smooth/pathology , Myometrium/pathologyABSTRACT
Morphological investigation of spontaneous abortions (SA) with normal and abnormal karyotype (220 specimens of the first trimester SA) was carried out. It included a histological pattern, apoptosis and proliferative activity. Estimation of apoptotic index (AI) was carried out in 7 cases of SA with normal karyotype and 7 cases of SA with pathological karyotype using the ApopTag Peroxidase Kits (Biotech, USA). The material of 4 therapeutic abortions was taken as the control group. The proliferative activity (PA) was determined as the rate of Ki-67-positive cytotrophoblast cells. PA was examined in SA groups with normal (15 cases) and pathological (15 cases) karyotypes and 20 cases of control group. Identical morphological signs of the development retardation of the villi were diagnosed among abortuses with normal karyotype and with aneuploidies. The diameter of the villi was bigger in aneuploid SA than in SA with normal karyotype (p > 0.05). The partial moles were diagnosed in triploidy, as it was expected. High level of AI and PA in the control group was revealed. In SA with pathological karyotype a high rate of AI accompanied a low level of PA. The AI and PA were low in SA with normal karyotype. However, the largest uncorrelation between AI and PA in extravillous trophoblast cells of SA with chromosomal anomalies were observed: the level of AI was higher than the level of PA. We suppose that the last one leads to the abruption of the ovum.
Subject(s)
Abortion, Spontaneous/pathology , Apoptosis/physiology , Chorion/pathology , Chromosome Aberrations , Trophoblasts/pathology , Abortion, Spontaneous/genetics , Apoptosis/genetics , Cell Proliferation , Female , Humans , Karyotyping , Ki-67 Antigen/metabolism , PregnancyABSTRACT
The uterus removed at 20-week pregnancy in a female with grave lupus erythematosus complicated with an antenatal fetus death was studied. Signs of arthrosis typical for late pregnancy were found in the spiral arteries of basal decidua in the zone of contact between mother tissue and trophoblast. Fetal death was due to massive thrombosis.
