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1.
Dokl Biochem Biophys ; 480(1): 131-134, 2018 May.
Article in English | MEDLINE | ID: mdl-30008092

ABSTRACT

The sodium selenite concentration that reduces the viability of Du-145 human prostate adenocarcinoma cells and F-9 mouse testicular teratocarcinoma cells was determined. We investigated the effect of sodium selenite on the mRNA expression level of the genes encoding mammalian selenocysteine-containing glutathione peroxidases and thioredoxin reductases (key antioxidant enzymes involved in the regulation of intracellular thiol redox balance), endoplasmic reticulum selenoproteins, and selenoproteins located in the testes and prostate.


Subject(s)
Gene Expression Regulation, Neoplastic/drug effects , Neoplasm Proteins/biosynthesis , Prostatic Neoplasms/metabolism , RNA, Messenger/biosynthesis , RNA, Neoplasm/biosynthesis , Selenoproteins/biosynthesis , Sodium Selenite/pharmacology , Testicular Neoplasms/metabolism , Cell Line, Tumor , Humans , Male , Prostatic Neoplasms/pathology , Testicular Neoplasms/pathology
2.
Biofizika ; 59(5): 941-5, 2014.
Article in Russian | MEDLINE | ID: mdl-25730977

ABSTRACT

The effect of a metabolic precursor of natural activator of mitochondrial ATP-dependent potassium channel (mitochondrial K+(ATP))--uridine on animal's endurance to physical stress was studied. The endurance was determined by recording the time period during which the rat loaded with a plummet of 20% of body weight can swim until physical exhaustion at 32 degrees C. It was found that highly resistant animals swam until exhaustion for 7.40 ± 0.35 min, whereas low resistant rats hold out 2.07 ± 0.10 min only. The injection of uridine influenced the swimming time of the animals, increasing it twofold in low-resistant rats. The effect of uridine was decreased by injection of inhibitors of mitochondrial K+(ATP) channel. It was found that the injection of uridine into low resistant rats increased the rate of potassium transport in mitochondria isolated from liver of these rats, and inhibitors of the channel prevent the channel activating effect of uridine. The role of mitochondrial K+(ATP) cannel in the formation of animal's resistance to physical stress and protection of tissues from hypoxia is discussed.


Subject(s)
Adenosine Triphosphatases/metabolism , Cation Transport Proteins/metabolism , Mitochondria, Liver/metabolism , Mitochondrial Proteins/metabolism , Physical Endurance/drug effects , Stress, Physiological/drug effects , Uridine/pharmacology , Animals , Ion Transport/drug effects , Male , Physical Conditioning, Animal , Physical Endurance/physiology , Potassium/metabolism , Rats , Rats, Wistar , Stress, Physiological/physiology
3.
Bull Exp Biol Med ; 151(1): 25-9, 2011 May.
Article in English | MEDLINE | ID: mdl-22442795

ABSTRACT

Activity of mitochondrial ATP-dependent potassium channel in rats with high genetically determined resistance to hypoxia was higher than in sensitive animals. Adaptation of low resistant rats to hypoxia was accompanied by activation of the channel, facilitation of potassium recycling in mitochondria, and a decrease in the rate of H2O2 formation. Our results indicate that mitochondrial ATP-dependent potassium channel plays an important role in the delayed mechanisms of animal's adaptation to hypoxia.


Subject(s)
Acclimatization/physiology , Hypoxia/metabolism , KATP Channels/metabolism , Potassium/metabolism , Adenosine Triphosphate/metabolism , Adenosine Triphosphate/pharmacology , Animals , Disease Resistance , Hydrogen Peroxide/metabolism , Hypoxia/genetics , Hypoxia/physiopathology , KATP Channels/agonists , Male , Mitochondria, Heart/drug effects , Mitochondria, Heart/metabolism , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Rats
4.
Biofizika ; 55(6): 1030-7, 2010.
Article in Russian | MEDLINE | ID: mdl-21268347

ABSTRACT

Changes in the rate of respiration and functioning of the ATP-dependent potassium channel the liver and heart mitochondria of one-, three-, eight-, and twenty four-month-old Wistar male rats have been investigated. It was shown that the activity of the channel in the mitochondria of both tissues in 24-months-old animals decreases more than three times, and the content of potassium, 1.5-2 times compared with young one-month-old rats. The changes occur against the background of age-related decrease of energy supply in mitochondria, the respiratory complex-I underganig the greatest changes upon aging. The decrease of channel activity may be the result of changes in channel sensitivity to modulators and a decrease in the expression of mitochondrial K(+)-transporting channel-protein with a molecular mass of 5.5 kDa upon aging found in this work. As a result, the functioning of not only the mitoK(ATP) but also the whole mitochondrial potassium cycle is impaired.


Subject(s)
Aging/metabolism , KATP Channels/physiology , Mitochondria, Heart/metabolism , Mitochondria, Liver/metabolism , Adenosine Triphosphate/biosynthesis , Animals , Biological Transport , Electron Transport Complex I/metabolism , Energy Metabolism , Male , Phosphorylation , Potassium/metabolism , Rats
5.
Klin Med (Mosk) ; 86(4): 49-50, 2008.
Article in Russian | MEDLINE | ID: mdl-18494287

ABSTRACT

Study of 297 patients with preparative methods of nontoxic nodular goiter and cytological diagnose "adenomatosis" with the use of artificial neural network (ANN) were analyzed. Malignant pathology could be supposed with 95.5% accuracy when we applied experience of trained ANN. Clinical, instrumental and cytologic criteria, which make possible to prognosticate degree of malignant process in thyroid gland parenchyma, were determined. Painless elastic nodules with sonographically iso- o hyperechogenic structure, with clear outline and cytologically determined adenomatosis in combination with colloid can be interpreted as benign lesion of thyroid gland.


Subject(s)
Goiter, Nodular/diagnosis , Neural Networks, Computer , Biopsy, Needle , Diagnosis, Differential , Follow-Up Studies , Goiter, Nodular/surgery , Humans , Predictive Value of Tests , Prognosis , Reproducibility of Results , Retrospective Studies
6.
Klin Lab Diagn ; (1): 37-9, 2008 Jan.
Article in Russian | MEDLINE | ID: mdl-18320618

ABSTRACT

By comparing the cytological and histological data on thyroid nodular lesions in 715 patients, the authors have arrived at the conclusion that with a cytological response of colloidal goiter and colloidal goiter with adenomatosis, the cancer detection rate is not greater than 4%. With a cytological response of adenomatosis with atypia and follicular tumor, a malignancy occurs in 9 to 13%. It is recommended that cytological data should be grouped by the type of a morphological classification of thyroid nodular diseases: colloidal goiter (with regression, proliferation, adenomatosis, cystic cavity); tumors (adenomatosis with atypia, follicular tumor); cancer.


Subject(s)
Goiter, Nodular/classification , Goiter, Nodular/pathology , Female , Goiter, Nodular/diagnosis , Humans , Male
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