ABSTRACT
Venezuelan equine encephalomyelitis, Eastern equine encephalomyelitis and Sindbis viruses, as well as members of various serological groups of alphaviruses were identified using synthesized molecular probes complementary to 3'-terminus of the molecule of the appropriate genomic RNA: 32P-single-stranded kDNA and E. coli-cloned 32P-double-stranded kDNA. A high species-specificity of kDNA-probes was demonstrated. The sensitivity of the method was 1 ng of viral RNA overlayed on the nitrocellulose filter.
Subject(s)
Alphavirus/isolation & purification , DNA Probes , DNA, Viral/genetics , DNA/genetics , Genetic Complementation Test , Genome, Viral , Alphavirus/genetics , Encephalitis Virus, Eastern Equine/genetics , Encephalitis Virus, Eastern Equine/isolation & purification , Encephalitis Virus, Venezuelan Equine/genetics , Encephalitis Virus, Venezuelan Equine/isolation & purification , Nucleic Acid Hybridization , RNA, Viral/genetics , Sindbis Virus/genetics , Sindbis Virus/isolation & purification , Transcription, GeneticABSTRACT
Administration to rodents (Syrian hamsters, mice, guinea pigs, rabbits) of minimal doses of attenuated strain 15 VEE (up to 20 ImD50) provided protection against respiratory challenge with a highly virulent strain of Venezuelan equine encephalomyelitis virus. The protection is observed in a wide range of doses (up to LD50 hundreds and even thousands).
Subject(s)
Encephalitis Virus, Venezuelan Equine/immunology , Encephalomyelitis, Venezuelan Equine/prevention & control , Respiratory Tract Infections/prevention & control , Rodent Diseases/prevention & control , Viral Vaccines/immunology , Animals , Cricetinae , Drug Evaluation, Preclinical , Encephalitis Virus, Venezuelan Equine/pathogenicity , Encephalomyelitis, Venezuelan Equine/etiology , Guinea Pigs , Immunization , Mesocricetus , Mice , Rabbits , Respiratory Tract Infections/etiology , Rodent Diseases/etiology , Serial Passage , Vaccines, Attenuated/administration & dosage , Vaccines, Attenuated/immunology , Viral Vaccines/administration & dosage , VirulenceABSTRACT
Immunization of Papio hamadryas with attenuated VEE virus (strain 15) causes seroconversion in 100% of animals. Postvaccinal reaction such as viremia and short-lasting fever occurs in response to the injection of 10(3) ImD50 in about 30% of animals. CNS lesion symptoms characterizing infection of Papio hamadryas with natural Trinidad strain do not occur in response to injection with attenuated strain.
