ABSTRACT
The influence of melaxen and valdoxan on the biochemiluminescence parameters, aconitate hydratase activity and citrate level in rats heart and liver during development of experimental hyperthyroidism has been investigated. Administration of these substances promoted a decrease of biochemiluminescence parameters, which had been increased in tissues of rats in response to the development of oxidative stress under hyperthyroidism. Aconitate hydratase activity and citrate concentration in rats liver and heart, growing at pathological conditions, changed towards control value after administration of the drugs correcting melatonin level. The results indicate the positive effect of valdoxan and melaxen on oxidative status of the organism under the development of experimental hyperthyroidism that is associated with antioxidant action of melatonin.
Subject(s)
Acetamides/pharmacology , Aconitate Hydratase/metabolism , Antioxidants/pharmacology , Citrates/metabolism , Free Radicals/antagonists & inhibitors , Hyperthyroidism/drug therapy , Melatonin/pharmacology , Animals , Drug Synergism , Free Radicals/metabolism , Heart/drug effects , Hyperthyroidism/chemically induced , Hyperthyroidism/metabolism , Injections, Intraperitoneal , Liver/drug effects , Liver/metabolism , Luminescent Measurements , Male , Oxidative Stress , Rats , TriiodothyronineABSTRACT
The effects of melaxen and valdoxan on the activity of glutathione antioxidant system and some NADPH-producing enzymes have been studied under conditions of experimental hyperthyroidism in rat heart. Under the action of these drugs, reduced glutathione (GSH) content increased as compared to values observed under the conditions of pathology. It has been established that the activities of glutathione reductase (GR), glutathione peroxidase (GP), glucose-6-phosphate dehydrogenase, and NADP isocitrate dehydrogenase (increased under pathological conditions) change toward the intact control values upon the introduction of both drugs. The influence of melaxen and valdoxan, capable of producing antioxidant effect, leads apparently to the inhibition of free-radical oxidation processes and, as a consequence, the reduction of mobilization degree of the glutathione antioxidant system.
Subject(s)
Acetamides/pharmacology , Antioxidants/pharmacology , Glutathione/metabolism , Heart/drug effects , Hyperthyroidism/drug therapy , Melatonin/pharmacology , NADP/metabolism , Animals , Glucosephosphate Dehydrogenase/metabolism , Glutathione/agonists , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Hyperthyroidism/chemically induced , Hyperthyroidism/metabolism , Isocitrate Dehydrogenase/metabolism , Male , Rats , Reactive Oxygen Species/antagonists & inhibitors , Reactive Oxygen Species/metabolism , TriiodothyronineABSTRACT
Investigation of glutathione antioxidant system activity and diene conjugates content in rats liver and blood serum at the influence of melaksen and valdoxan under experimental hyperthyroidism (EG) has been revealed. It has been established that the activities of glutathione reductase (GR), glutathione peroxidase (GP) and glutathione transferase (GT), growing at pathological conditions, change to the side of control value at these substunces introduction. Reduced glutathione content (GSH) at melaxen and valdoxan action increased compared with values under the pathology, that, obviously, could be associated with a reduction of its spending on the detoxication of free radical oxidation (FRO) toxic products. Diene conjugates level in rats liver and blood serum, increasing at experimental hyperthyroidism conditions, under introduction of melatonin level correcting drugs, also approached to the control meaning. Results of the study indicate on positive effect of melaxen and valdoxan on free radical homeostasis, that appears to be accompanied by decrease of load on the glutathione antioxidant system in comparison with the pathology.
