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Anal Chem ; 80(18): 6881-7, 2008 Sep 15.
Article in English | MEDLINE | ID: mdl-18698800

ABSTRACT

A microfabricated device has been developed in which electrospray ionization is performed directly from the corner of a rectangular glass microchip. The device allows highly efficient electrokinetically driven separations to be coupled directly to a mass spectrometer (MS) without the use of external pressure sources or the insertion of capillary spray tips. An electrokinetic-based hydraulic pump is integrated on the chip that directs eluting materials to the monolithically integrated spray tip. A positively charged surface coating, PolyE-323, is used to prevent surface interactions with peptides and proteins and to reverse the electroosmotic flow in the separation channel. The device has been used to perform microchip CE-MS analysis of peptides and proteins with efficiencies over 200,000 theoretical plates (1,000,000 plates/m). The sensitivity and stability of the microfabricated ESI source were found to be comparable to that of commercial pulled fused-silica capillary nanospray sources.


Subject(s)
Electrophoresis, Capillary/methods , Glass/chemistry , Microfluidic Analytical Techniques/methods , Spectrometry, Mass, Electrospray Ionization/methods , Adsorption , Animals , Cattle , Electrophoresis, Capillary/instrumentation , Microfluidic Analytical Techniques/instrumentation , Peptides/isolation & purification , Proteins/isolation & purification , Proteomics , Reproducibility of Results , Sensitivity and Specificity , Spectrometry, Mass, Electrospray Ionization/instrumentation , Surface Properties
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