[Comparative characteristics of nuclear polyhedrosis virus isolates from gypsy moth Lymantria dispar L]. / Sravnitel'naia kharakteristika izoliatov virusa iadernogo poliédroza neparnogo shelkopriada Lymantria dispar L.

Eight strains of Gypsy moth nuclear polyhedrosis virus (NPV) were isolated from dead larvae in Kirghizia and Tyumen and Novosibirsk districts. The strains were characterized in terms of polyhedron morphology, biological activity, virus production, and restriction analysis of DNA. The isolated strains did not differ in size or shape of polyhedrons. NPV strain NSHN-2 was characterized by the highest biological activity (LD50 = 1.2 +/- 0.4) x 10(4) and maximum production: 7.3 x 10(8) PIB/larva in comparison with the rest isolated strains. This strain is proposed for the manufacture of an insecticide.

[Quantitative determination of the infectivity of nuclear polyhedrosis virus DNA on honeycomb moth (Galleria mellonella) larvae]. / Kolichestvennoe opredelenie infektsionnosti DNK virusa iadernogo poliédroza na lichinkakh bol'shoi voshchinnoi moli (Galleria mellonella).

The possibility of using honeycomb moth larvae for titration of nuclear polyhedrosis virus (NPV) infectious DNA and determinations of transfection effectiveness was studied. Honeycomb moth larvae were shown to be a sensitive system for NPV DNA titration. DEAE-dextran used as a protector increased NPV DNA infectivity 1000-fold, LD50 in this instance being 2 X 10(8) molecules per larva. The method of NPV DNA infectivity determinations by the number of larvae with polyhedreae in the fatty tissue is more sensitive than infectivity determinations by the number of dead larvae and permits titrations of low DNA concentrations. The curve of DNA titration in the presence of DEAE-dextran by the number of larvae with polyhedrae in the fatty tissue allows to quantitate native DNA within the range of 0.01 to 5 micrograms/ml.

[Synthesis of virus-specific products following introduction of tobacco mosaic virus RNA pereparations and the native virus into acetabularia]. / Izuchenie sinteza virusspetsificheskikh produktov posle vvedeniia v atsetabuliariiu preparatov RNK virusa tabachnoi mozaiki i nativnogo virusa.

The possibility to synthesize the viral-specific products after microinjection of Tobacco mosaic virus (TMV) preparations and the TMV RNA into the single-celled seaweed Acetabularia was studied. The accumulation of the newly synthesized protein and double-stranded RNA 24 hours after injection of TMV RNA and native virus preparations was demonstrated by immunological and immunofluorescent methods. The virus titer sharply dropped 3--4 hours after introduction into Acetabularia and in 48 hours it reached a maximum level. The presented data showed the possibility of TMW RNA replication and translation involving formation of viral-specific proteins and the production of a virus of full value in the Acetabularia cell.

[RNA synthesis gradients in the nucleus-free fragments of Acetabularia mediterranea under conditions of local illumination]. / Gradienty sinteza RNK u bez'iadernykh fragmentov Acetabularia meditarranea v usloviiakh lokal'nogo osveshcheniia.

The intensity of RNA synthesis was studied in different regions of anuclear fragments of the Acetabularia mediterranea stem under their local illumination. The local illumination was shown to activate RNA synthesis and formation of distal-medial gradients of this synthesis in the illuminated regions of the fragments to a much greater extent than in their darkened regions. The formation of the gradient in the illuminated region took place even if the growth in this region was insignificant and was not accompanied by the cap formation. The results obtained suggests the absence of obligatory correspondence between the ability of morphogenesis and the gradient of RNA synthesis.

[Study of the stability of morphogenetic substances in Acetabularia]. / Izuchenie stabil'nosti morfogeneticheskikh veshchestv u atsetabuliarii.

It was shown by means of interspecific transplantation that the morphogenetic substances in the A. crenulata cytoplasm kept their ability to determine the species specific morphogenesis in the A. mediterranea fragments during not less than 30 days. The isolation of the A. crenulata cytoplasm from the cell resulted in the complete inactivation of morphogenetic substances within 2--3 hrs. The removal of chloroplasts from the injected cytoplasm did not change its morphogenetic activity. The cytoplasm treatment by exogenous ribonuclease accelerated the inactivation of morphogenetic substances; this suggests its ribonucleic nature.