ABSTRACT
The mechanism promoting the nonspecific action of antigens obtained from S. flexneri and S. sonnei by a sparing method has been studied. These antigens stimulate the T- and B- systems of immunity, that is followed by activation of myelopoiesis and the humoral protective factors of the body, which seems to underlie the formation of resistance to infection caused by nonspecific microorganisms.
Subject(s)
Antigens, Bacterial/immunology , Immunity, Innate , Shigella flexneri/immunology , Shigella sonnei/immunology , Animals , Antibodies, Bacterial/analysis , Antibody-Producing Cells/immunology , Bone Marrow/immunology , Colony-Forming Units Assay , Hemagglutination Tests , Hemolytic Plaque Technique , Immunization , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , T-Lymphocytes/immunologyABSTRACT
In experiments on gamma-irradiated hamsters it is shown that antigens, obtained from Sh. flexneri and sonnei by the sparing method, have a radioprotective therapeutic action. The antigens exhibit the immunostimulating activity which is perhaps one of the aspects of the mechanism involved in the formation of resistance to infectious complications in irradiated animals and of the favourable radioprotective effect.
Subject(s)
Antigens, Bacterial/administration & dosage , Radiation Injuries, Experimental/therapy , Shigella/immunology , Acute Disease , Animals , Cesium Radioisotopes , Cobalt Radioisotopes , Cricetinae , Gamma Rays , MaleSubject(s)
Antigens, Bacterial/administration & dosage , Bacterial Vaccines/administration & dosage , Radiation-Protective Agents , Shigella flexneri/immunology , Shigella sonnei/immunology , Animals , Female , Gamma Rays , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Radiation Injuries, Experimental/prevention & control , Radiation ToleranceABSTRACT
Of the live Sh. Flexner 2a and Sonne soluble antigens containing glucide-lipoid-protein complex, characterized by low toxicity, a high yield of dry substances, and the capacity to induce immunity production in mice after subcutaneous and oral immunization, were obtained by salt extraction and physical disintegration. Local antigen application into eye mucosa of guinea pigs produced local resistance against homologous causative agents and also stimulated production of the highest level of specific antibodies at the site of administration in oral and conjunctival vaccination. A marked antigenic-immunological activity of soluble shigella antigens in local administration permits to refer them to the group of protective ones and to recommend their further study in the capacity of preparations for enteral vaccination against dysentery.
