[Morphometric characteristic of the subepithelial connective tissue of the rabbit gingiva under the experimental gingivitis on the background of hypercholesterinemia].

The morphologic substrate of the gingival mucosa membrane during the increase of the proatherogenic lipids concentration and the damage the gingiva under the influence of the atherogenic diet was studied in experiment. By the planimetric method the middle indices of the number of capillaries, arterioles and venules and as well the quantities of immunocompetent cells (macrophages and lymphocytes) in the gingival subepithelial connective tissue were defined. The experiments were carried out on rabbits (race "Shinshila"). Animals were on atherogenic ration (cholesterol 0,3 g/kg with vegetables). The experimental gingivitis was modeled by ligation of a silk ligature around the dental neck of the tree superior basal teeth after peeling the gingiva. It was established that under the gingivitis developing against the background of hypercholesterinemia in the subepithelial layer of the gingival mucosa the degree of the vascularisation distinctly diminished, owing to the decrease the number of arteriols and partly capillaries and transformation their walls--the thin-walled capillaries with obliterated, deformated lumen appeared. Endotheliocites with changed structure, ordinary pycnosis of nuclei which creates the necessary prereqvisites for the disturbance of trophicity and progression of the inflammatory process.

[The peculiarity of the regeneration of the gingival mucosa after the operation of the tooth extraction].

The indices of the mitotic activity, the quantity of the double-nucleated cells and the dying nucleus coefficient in the basal and spinous layers of the rat multilayer flat epithelium after the extraction a tooth were studied. The experiments were carried out on 45 nonlinear white mature rats - males. After peeling the gingiva around the dental neck we extract three basal teeth. In the epithelial regenerates we counted amount of the mitotic figures (prophase, metaphase, anaphase, telophase) in the basal and spinous cells, double-nucleated cells in the spinous layer and the number of the dying nucleus (picnosis, karyolisis) among 5 000 cells. It was established that the tissue defect was filled up owing to the intensification of the mitotic activity in the germinative layer, namely, dew new cells generation. In spite of the rise the quantity of dying nucleus, the number of the dividing cells exceeds the number of the dying owns, what provides the reparation of the multilayer flat gingival epithelium in the region of the wound.

[Evaluation of the preserved skin graft viability by O2 consumption test].

This study provides a description of the functional changes occurring in rat skin grafts preserved at 4 C in natural honey during 3, 7, 14, 30 days. To determine of the preserved skin viability we used the physiological test by measurement of O2 consumption rate in the culture media. O2 consumption measurement provided by Zieger MA, et al. For control was used normal and dead skin samples. Normal skin O2 consumption rate was considered as 100% viability, and dead skin - 0%. Preserved skin O2 consumption rate per 3, 7, 14, 30 days was - 72%, 38%, 15%, 8% respectively. Natural honey is antiseptic and cryoprotective material. Based on our investigation we can conclude that preservation method--meliteration is effective storage procedure for transplantation means (satisfactory viability preserved about 2-3 weeks). At the same time O2 consumption test gives us objective information about tissue viability potential. This method is very simple and useful for scientific investigation and practical medicine.