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1.
J Virol Methods ; 126(1-2): 91-100, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15847924

ABSTRACT

The present study describes the phenotypic and genotypic features of seven individual growth transformed, euploid-diploid EBV+ human B cell lines arisen spontaneously in vitro. The lines, obtained under general and standard culture conditions (un-manipulated), from seven individual bone marrow samples of 18 healthy young adults, Caucasian, of both sexes, display many traits of normal B cells and represent a mixture of EBV infected latently (latency type III) and producer cells (5-16% VCA+ by immunofluorescence) releasing seven individual different viral strains [Fruscalzo et al., 2001. DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication. Gene 265, 165-173] similar to the B95-8 genotype as shown by results of Southern blot of BamHI-digested DNA fragment. These tests were planned to characterize more fully this panel of new bone marrow cell lines sharing normal B cell traits.


Subject(s)
B-Lymphocytes/cytology , B-Lymphocytes/virology , Bone Marrow Cells/cytology , Cell Line, Transformed , Herpesvirus 4, Human/genetics , Virus Latency , B-Lymphocytes/immunology , Cell Cycle , Cell Proliferation , Cell Transformation, Viral , Female , Gene Expression , Gene Rearrangement, B-Lymphocyte , Genome, Viral , Humans , Male , RNA, Messenger/analysis , RNA, Viral/analysis
2.
Rev. bras. cir ; 83(6): 243-50, nov.-dez. 1993. ilus
Article in Portuguese | LILACS | ID: lil-127947

ABSTRACT

A blefarofimose é uma deformidade congênita caracterizada pela diminuiçäo da fenda palpebral nos diâmetros horizontal e vertical, sem a participaçäo da borda palpebral. Caracteriza-se por uma tríade clássica constituída de epicanto, telecanto e ptose palpebral, além do caráter hereditário. Os autores apresentam sete pacientes, todos submetidos à correçäo cirúrgica pela técnica de Mustardé para epicanto e telecanto. A ptose foi corrigida pela ressecçäo ou plicatura do músculo levantador da pálpebra ou suspensäo frontal por fascia lata. Foram realizados tempos cirúrgicos únicos ou em 2 tempos, sendo em dois tempos o mais acertado, com resultados satisfatórios


Subject(s)
Humans , Male , Female , Child, Preschool , Adolescent , Adult , Blepharophimosis/surgery , Surgical Procedures, Operative
3.
Rev. bras. cir ; 83(3): 103-10, maio-jun. 1993. ilus
Article in Portuguese | LILACS | ID: lil-127901

ABSTRACT

Descriçäo das técnicas cirúrgicas dos retalhos de Limberg e subcutâneo para reconstruçäo de pequenos defeitos cirúrgicos da face. Eles defedem a opiniäo de que, com estes dois retalhos apenas, a grande maioria dos problemas de pequenos defeitos de pele da face poderá ser solucionada, apresentando bons resultados estéticos


Subject(s)
Humans , Male , Female , Surgery, Plastic/methods , Face/surgery , Surgical Flaps/methods , Surgical Flaps/physiology
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