ABSTRACT
The Macroscopic, histological and immunohistochemical aspects of lung acinar adenocarcinoma and the presence of nodules in the abdominal cavity of an adult female bovine are reported. In the necropsy analysis samples were collected from the: lung, heart, spleen, liver, pancreas, kidney, uterus, intestine, brain, and from nodules found in the lung and abdominal cavity, which were routinely processed to be stained by hematoxylin-eosin and for an immunohistochemistry exam with the antibodies: cytokeratin (dilution 1:200 µL) and vimentin (dilution 1:1000 µL). The histopathological examination revealed neoplastic epithelial cells with acini formation. The immunohistochemical examination of the tumor cells showed positive marking for cytokeratin and the absence of marking for vimentin. According to anatomical, morphological, and histopathological findings, as well as the result of the immunohistochemical examination, the tumor was characterized as lung acinar adenocarcinoma.
Se relatan los aspectos macroscópicos, histológicos e inmunohistoquímicos de un adenocarcinoma acinar pulmonar y la presencia de nódulos en la cavidad abdominal en una hembra bovina adulta. En el análisis necroscópico fueron colectados fragmentos de: pulmón, corazón, bazo, hígado, páncreas, riñón, útero, intestino, encéfalo y de los nódulos hallados en pulmón y cavidad abdominal, los cuales fueron procesados rutinariamente para ser teñidos mediante Hematoxilina-Eosina y para examen inmunohistoquímico con los anticuerpos: citoqueratina (con dilución 1:200 µL) y vimentina (dilución 1:1000 µL). El examen histopatológico reveló células epiteliales neoplásicas con formación de acinos. El examen inmunohistoquímico de las células neoplásicas demostró marcación positiva para citoqueratina y ausencia de marcación para vimentina. De acuerdo con los hallazgos anatómicos, morfológicos, histopatológico, y el resultado del examen inmunohistoquímico, se logró caracterizar el tumor como adenocarcinoma acinar pulmonar.
Subject(s)
Immunochemistry , Lung , Neoplasm Metastasis , NeoplasmsABSTRACT
Aural plaques occur on the skin of the medial surface of the pinnae of horses. In this study the presence of Equus caballus papillomavirus (EcPV)-3 and -4 DNA was assessed in 45 such plaques using a 'touchdown' PCR. Papillomaviruses (PVs) were detected in 62.3% (28/45) of samples: EcPV-3 and -4 DNA in 8.89% (4/45) and 37.78% (17/45) of samples, respectively, with 15.56% (7/45) of samples exhibiting co-infection. Viral DNA was not detected in 37.78% (17/45) of samples, suggesting the possible existence of other equine PVs. Neither EcPV-3 nor -4 were detected in negative control skin. This study is the first to evaluate the prevalence of these two viruses in equine aural plaques.
Subject(s)
Ear Diseases/veterinary , Horse Diseases/virology , Papillomaviridae/classification , Papillomavirus Infections/veterinary , Polymerase Chain Reaction/veterinary , Skin Diseases, Viral/veterinary , Animals , DNA, Viral/genetics , Ear Diseases/virology , Horse Diseases/diagnosis , Horses , Papillomaviridae/genetics , Papillomaviridae/isolation & purification , Papillomavirus Infections/diagnosis , Papillomavirus Infections/virology , Polymerase Chain Reaction/methods , Sensitivity and Specificity , Skin Diseases, Viral/diagnosisABSTRACT
Papillomaviruses (PVs) infect a wide range of animal species and show great genetic diversity. To date, excluding equine sarcoids, only three species of PVs were identified associated with lesions in horses: Equus caballus papillomavirus 1 (EcPV1-cutaneous), EcPV2 (genital) and EcPV3 (aural plaques). In this study, we identified a novel equine PV from aural plaques, which we designated EcPV4. Cutaneous samples from horses with lesions that were microscopically diagnosed as aural plaques were subjected to DNA extraction, amplification and sequencing. Rolling circle amplification and inverse PCR with specific primers confirmed the presence of an approximately 8 kb circular genome. The full-length EcPV4 L1 major capsid protein sequence has 1488 nucleotides (495 amino acids). EcPV4 had a sequence identity of only 53.3%, 60.2% and 51.7% when compared with the published sequences for EcPV1, EcPV2 and EcPV3, respectively. A Bayesian phylogenetic analysis indicated that EcPV4 clusters with EcPV2, but not with EcPV1 and EcPV3. Using the current PV classification system that is based on the nucleotide sequence of L1, we could not define the genus of the newly identified virus. Therefore, a structural analysis of the L1 protein was carried out to aid in this classification because EcPV4 cause lesion similar to the lesion caused by EcPV3. A comparison of the superficial loops demonstrated a distinct amino acid conservation pattern between EcPV4/EcPV2 and EcPV4/EcPV3. These results demonstrate the presence of a new equine PV species and that structural studies could be useful in the classification of PVs.
