[Persistent infection caused by the Coxsackie B3 virus in adult mice]. / Persistentnaia infektsiia, vyzyvaemaia virusom Koksaki B3 u vzroslykh myshei.

A model of persistent infection with Coxsackie B-3 virus was developed in adult mice with clinical manifestations of the disease and long-term (up to 13 months) excretion of the causative agent. The method of multiple organ cultures was shown to be suitable for isolation of the persisting enterovirus. The presence of persistent infection was confirmed by the detection of IgM antibody in repeated daily examinations of the animals for 4 months. It seems to be expedient to use this model for investigations of the pathogenesis and methods of treatment of persistent Coxsackie B-3 infection in experimental animals.

[Gliotoxic properties of multiple sclerosis and polyradiculoneuritis serum detectable in brain organ culture]. / Gliotoksicheskie svoistva syvorotki krovi u bol'nykh rasseiannym sklerozom i poliradikulonevritom, vyiavaliaemye v organnoi kul'ture mozga.

The blood serum of patients with disseminated sclerosis and polyradiculoneuritis was tested for gliotoxic effect in organ cultures of the brain of newborn cotton rats (Sygmodon hispidus). This effect was revealed in 18 out of 25 patients with disseminated sclerosis and in 9 out of 16 patients with polyradiculoneuritis. As for other diseases the gliotoxic effect was observed only in 3 out of 41 patients examined. The data obtained confirm the significance of the allergic mechanisms in the pathogenesis of demyelinization. It is suggested that the test for the serum gliotoxic effect should be used as a diagnostic criterion of the process activity in patients with disseminated sclerosis.

[Biological characteristics and the morphology of human and animal central nervous system tissue in organ culture]. / Biologicheskaia kharakteristika i morfologiia tkani tsentral'noi nervnoi sistemy cheloveka i zhivotnykh v organnoi kul'ture.

It is shown that in using the method of multiple organ cultivation, the tissues of the CNS in the human and animal embryo preserve their organotypical characteristics. The use of a cartilage plate increases the viability of the tissues and the duration of its maintenance in cultures. During the process of explantation the cultivated tissue undergoes some morphological modifications. The data obtained are discussed in the light of a possible use of the CNS cultures for experimental purposes.

[Determination of the gliotoxie and demyelinating properties of serum in organ cultures of brain in experimental allergic encephalomyelitis]. / Opredelenie gliotoksicheskikh i demieliniziruiushchikh svoistv syvorotki krovi v organnoi kul'ture mozga pri éksperimental'nom allergicheskom éntsefalomielite

The report contains some results of a study of the blood serum taken from guinea pigs and macacus rhesus with allergic encephalomyelitis to a glyotoxic and demyelinizing effect in an organ culture of the brain in newborn rats and adult macacus rhesus. It was demonstrated that the test has a high specificity to a glyotoxic effect of the blood serum in experimental allergic encephalomyelitis and a less specificity to demyelinization in vitro. The authors describe in detail the method of studying the serum in organ brain cultures.