[The effect of antithymocyte serum on the natural cytotoxicity of C3HA mouse splenocytes in the early periods after 20-methylcholanthrene administration]. / Izuchenie vliianiia antitimotsitarnoi syvorotki na estestvennuiu tsitotoksichnost' splenotsitov myshei C3HA na rannikh srokakh posle vvedeniia 20-metilkholantrena.

The role of T-lymphocytes in natural cytotoxicity of splenocytes of C3HA mice after a single injection of 20-methylcholanthrene (20-MC) was investigated. A splenocyte suspension was treated with anti-T-cell serum and complement. This treatment was not shown to exert influence on the natural cytotoxicity of splenocytes within 1-13 days after injecting 20-MC.

[The immunomodulation of the natural killer activity of the splenocytes in C3HA mice during hepatoma 22a growth]. / Immunomoduliatsiia estestvennoi killernoi aktivnosti splenotsitov myshei C3HA v usloviiakh rosta gepatomy 22a.

A single injection of C3HA mice with various immunomodulators-ds-RNA, thymogene (TM) and cyclophosphamide (CY)--performed one day before transplantation of syngeneic hepatoma 22a cells led to a decrease in the tumor growth rate. The most prominent effect was found following the CY treatment. The NK cell activity estimated per spleen of mice treated with ds-RNA and TM was seen increased in comparison with the control mice not given the modulators. The rate of tumor growth was due probably to this fact. The protective effect of CY may be accounted for by a direct action of this agent on tumor cells.

[The effect of macrophages on the natural killer activity of the splenocytes of C3HA mice in the early stages of 20-methylcholanthrene-induced carcinogenesis]. / Vliianie makrofagov na estestvennuiu killernuiu aktivnost' splenotsitov myshei C3HA na rannikh stadiiakh kantserogeneza, vyzvannogo 20-metilkholantrenom.

The influence of macrophages on NK cell activity of C3HA mice on 1, 7 and 13 days after single i. m. injection of 20-methylcholanthren was investigated. It is shown that macrophages significantly stimulate this activity on the 13th day after carcinogen application.

[The effect of anti-T sera on the natural killer activity of the splenocytes in C3HA mice at different times after the transplantation of hepatoma 22a cells]. / Vliianie anti-T-syvorotok na estestvennuiu killernuiu aktivnost' splenotsitov myshei C3HA v rannie sroki posle transplantatsii kletok gepatomy 22a.

Suspension of splenocytes of control C3HA mice and mice examined early after transplantation of mouse hepatoma 22a cells were fractionated by treatment with anti-T-sera (anti-thymocytes, antibrain and anti-suppressor-T-cells). This treatment leads to various changes in NK-activity due to elimination of different subpopulations of T-lymphocytes. This variability may be associated with the presence of two or more types of suppressor cells able to influence the NK-cells directly or by means of other immunocompetent cells.

[Natural killer activity of the splenocytes in C3HA strain mice during 20-methylcholanthrene-induced carcinogenesis]. / Estestvennaia killernaia aktivnost' splenotsitov myshei linii C3HA v usloviiakh kantserogeneza, vyzvannogo 20-metilkholantrenom.

The induction of tumor in C3HA mice after intramuscular injection with 20-methylcholanthrene is accompanied by a decrease in natural antitumor resistance. This conclusion is based on the observation of the decreased natural killer activity per total number of splenocytes, from the time of carcinogen application till the appearance of tumor nodes.

[Intraspecies identification of cultured murine cells by using H-2 antigen typing]. / Vnutrividovaia identifikatsiia kul'tiviruemykh kletok myshi s pomoshch'iu tipirovaniia H-2-antigenov.

17 mouse cell lines have been screened with specific sera against H-2 antigens. All the cell lines tested expressed H-2 antigens characteristic of the donor haplotype. The data obtained indicate that H-2 typing of cultured mouse cells can be used as an approach to control their intraspecies diversity.

[Change of the hereditary properties of Amoeba proteus during micrurgic intervention at the time of mitosis (metaphase ane early anaphase)]. / Izmenenie nasledstvennykh svoistv Amoeba proteus pri mikrurgicheskom vozdeistvii vo vremia deleniia (metafaza i ranniaia anafaza)

Studies have been made on viability and hereditary properties of the progeny of amoebae after micrurgical coercions (sectioning of cells, suction of cytoplasm) during metaphase and early anaphase. The progeny of the fragments as well as of the cells with the reduced bulk of the cytoplasm exhibits unusual methionine resistance. Possible mechanism of the observed phenomemon are discussed.