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2.
Klin Lab Diagn ; 61(5): 286-288, 2016.
Article in Russian | MEDLINE | ID: mdl-31529907

ABSTRACT

The kidneys are one of main homeostatic organs participating in regulation of concentration of osmotic active substances, ionic composition and acid-base balance, in maintenance of volume of fluid of internal medium of organism fulfilling excretory function. The analysis of urine is largely applied in laboratory practice for detecting different pathological conditions. The actual study was organized to develop simple, accessible and economic mode of evaluation of functional condition of kidneys in patients with somatic diseases, detection of their early dysfunction and timely implementation of adjustment. The study was carried out on sampling of 78 patients: 58 persons with inflammatory diseases of intestine (ulcerous colitis, Crohn's disease) and 20 healthy persons (volunteers). The proposed technique is based on detection of total pool of metabolites in day urine of patients. The spectrophotometer is applied to detect main groups of metabolites of urine within range of wavelength 210-300 nm. The excess amount of pathologic components, toxically effecting and affecting homeostasis of internal medium of organism, is detected under establishment of total optical density. Its value is ≥30 standard unit and also sum of optical density within range 210-230 and 270-290 nm, equal 10 and 7 standard units correspondingly testify presence of glomerular and tubular apparatus of kidneys.

3.
Bull Exp Biol Med ; 159(1): 24-8, 2015 May.
Article in English | MEDLINE | ID: mdl-26033582

ABSTRACT

We studied specific features of erythrocyte membrane response to short-term occlusion of the brachial artery in patients with cardiovascular pathology. Under ischemic conditions, processes of sorption were primarily intensified in patients with effort angina and processes of hemoglobin binding with erythrocyte membrane predominated in patients with essential hypertension. These changes in the cell membrane were related to modulation of aggregation properties of erythrocytes (in patients with angina) and plasminogen activity (in patients with essential hypertension). They can also be associated with changes in glucose levels (effort angina) and uric acid (essential hypertension) whose effects can be significantly modified by other endogenous factors.


Subject(s)
Angina, Stable/blood , Coronary Disease/blood , Erythrocyte Membrane/physiology , Adsorption , Adult , Arm/blood supply , Blood Viscosity , Brachial Artery , Constriction , Fibrinogen/analysis , Hemoglobins/analysis , Humans , Ischemia/etiology , Lipids/blood , Male , Malondialdehyde/blood , Middle Aged , Nucleotides/blood , Plasminogen/analysis , Platelet Adhesiveness , von Willebrand Factor/analysis
4.
Eksp Klin Gastroenterol ; (5): 21-7, 2009.
Article in Russian | MEDLINE | ID: mdl-20201301

ABSTRACT

Investigation of erythrocyte membrane in patients with Crohn's disease detected the changes in asymmetry of its molecular components, abnormalities in normal functioning of membrane-associated enzymes and energy-dependent processes in the cell. The mechanism of damaging effect on the cell membrane was caused by the excessive blood accumulation of a composite complex of low-molecular substances, compounds of quinone and carbohydrate nature, peptides, modified forms of nucleotides. The revealed changes make it possible to assess the severity of endogenous intoxication and cell membrane disorder, which take place not only in the course of the disease acute attack, but also retain during the period of incomplete clinical and endoscopic remission.


Subject(s)
Crohn Disease/metabolism , Crohn Disease/pathology , Erythrocyte Membrane/metabolism , Erythrocyte Membrane/pathology , Adolescent , Adult , Female , Humans , Magnetic Resonance Spectroscopy/methods , Male , Middle Aged
5.
Bull Exp Biol Med ; 137(4): 373-4, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15452606

ABSTRACT

The composition of the protein-peptide complex of cryopreserved human fetal tissues and juvenile tissues of newborn rabbits was studied by spectrophotometric and chromatographic methods (liquid, thin layer, and reaction paper chromatography).


Subject(s)
Fetus/chemistry , Peptides/analysis , Proteins/analysis , Tissue Extracts/chemistry , Animals , Cryopreservation , Fetus/anatomy & histology , Gestational Age , Humans , Rabbits
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