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Mikrobiologiia ; 83(4): 451-5, 2014.
Article in Russian | MEDLINE | ID: mdl-25844456

ABSTRACT

In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, andmorphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach, combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters; for identification of microorganisms isolated from environmental objects and clinical samples are discussed.


Subject(s)
Bacteria , Bacterial Typing Techniques/methods , Ecosystem , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Bacteria/classification , Bacteria/genetics , Bacteria/isolation & purification , Bacteria/metabolism , RNA, Bacterial/metabolism , RNA, Ribosomal, 16S/metabolism
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