1.
Mikrobiologiia
; 83(4): 451-5, 2014.
Article
in Russian
| MEDLINE
| ID: mdl-25844456
ABSTRACT
In order to confirm the taxonomic position of environmental strains determined based on their biochemical, cultural, andmorphological characteristics, molecular genetic identification was carried out. A number of problems in identification of microorganisms were shown to be associated with contamination of the cultures in the course of isolation. Advantages of a comprehensive approach, combining 16S rRNA gene sequencing with a set of biochemical, cultural, and morphological parameters; for identification of microorganisms isolated from environmental objects and clinical samples are discussed.