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1.
Br J Pharmacol ; 171(2): 403-14, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24117106

ABSTRACT

BACKGROUND AND PURPOSE: L-DOPA is generally considered to alleviate the symptoms of Parkinson's disease by its conversion to dopamine. We have proposed that DOPA is itself a neurotransmitter in the CNS. However, specific receptors for DOPA have not been identified. Recently, the gene product of ocular albinism 1 (OA1) was found to exhibit DOPA-binding activity. Here, we have investigated whether OA1 is a functional receptor of DOPA in the nucleus tractus solitarii (NTS). EXPERIMENTAL APPROACH: We examined immunohistochemical expression of OA1 in the NTS, and the effects of DOPA microinjected into the depressor sites of NTS on blood pressure and heart rate in anaesthetized rats, with or without prior knock-down of OA1 in the NTS, using shRNA against OA1. KEY RESULTS: Using a specific OA1 antibody, OA1-positive cells and nerve fibres were found in the depressor sites of the NTS. OA1 expression in the NTS was markedly suppressed by microinjection into the NTS of adenovirus vectors carrying the relevant shRNA sequences against OA1. In animals treated with OA1 shRNA, depressor and bradycardic responses to DOPA, but not those to glutamate, microinjected into the NTS were blocked. Bilateral injections into the NTS of DOPA cyclohexyl ester, a competitive antagonist against OA1, suppressed phenylephrine-induced bradycardic responses without affecting blood pressure responses. CONCLUSION AND IMPLICATIONS: OA1 acted as a functional receptor for DOPA in the NTS, mediating depressor and bradycardic responses. Our results add to the evidence for a central neurotransmitter role for DOPA, without conversion to dopamine.


Subject(s)
Bradycardia/chemically induced , Dihydroxyphenylalanine/pharmacology , Dopamine Agents/pharmacology , Heart Rate/drug effects , Receptors, G-Protein-Coupled/metabolism , Solitary Nucleus/drug effects , Animals , Blotting, Western , CHO Cells , COS Cells , Chlorocebus aethiops , Cricetinae , Cricetulus , Dependovirus/genetics , Gene Transfer Techniques , Hypothalamus/physiology , Immunohistochemistry , Male , Plasmids/genetics , Rats , Rats, Wistar , Real-Time Polymerase Chain Reaction , Receptors, G-Protein-Coupled/genetics
2.
Neuroscience ; 161(3): 743-52, 2009 Jul 07.
Article in English | MEDLINE | ID: mdl-19368896

ABSTRACT

Calcium acts as an important second messenger in the intracellular signal pathways in a variety of cell functions. Strictly controlled intracellular calcium is required for proper neurite outgrowth of developing neurons. However, the molecular mechanisms of this process are still largely unknown. Neuronal calcium sensor-1 (NCS-1) is a high-affinity and low-capacity calcium binding protein, which is specifically expressed in the nervous system. NCS-1 was distributed throughout the entire region of growth cones located at a distal tip of neurite in cultured chick dorsal root ganglion neurons. In the central domain of the growth cone, however, NCS-1 was distributed in a clustered specific pattern and co-localized with the type 1 inositol 1,4,5-trisphosphate receptor (InsP(3)R1). The pharmacological inhibition of InsP(3) receptors decreased the clustered specific distribution of NCS-1 in the growth cones and inhibited neurite outgrowth but did not change the growth cone morphology. The acute and localized loss of NCS-1 function in the growth cone induced by chromophore-assisted laser inactivation (CALI) resulted in the growth arrest of neurites and lamellipodial and filopodial retractions. These findings suggest that NCS-1 is involved in the regulation of both neurite outgrowth and growth cone morphology. In addition, NCS-1 is functionally linked to InsP(3)R1, which may play an important role in the regulation of neurite outgrowth.


Subject(s)
Growth Cones/physiology , Inositol 1,4,5-Trisphosphate Receptors/metabolism , Neurites/physiology , Neuronal Calcium-Sensor Proteins/metabolism , Neuropeptides/metabolism , Animals , Boron Compounds/administration & dosage , Calcium/metabolism , Calcium Channel Blockers/administration & dosage , Cells, Cultured , Chick Embryo , Fluorescent Antibody Technique , Ganglia, Spinal/cytology , Ganglia, Spinal/drug effects , Ganglia, Spinal/physiology , Growth Cones/drug effects , Immunoblotting , Immunohistochemistry , Inositol 1,4,5-Trisphosphate Receptors/antagonists & inhibitors , Microscopy, Confocal , Microscopy, Fluorescence , Neurites/drug effects , Pseudopodia/physiology , Ryanodine/administration & dosage , Ryanodine Receptor Calcium Release Channel/metabolism , Time Factors
3.
Neurosci Lett ; 442(2): 114-7, 2008 Sep 12.
Article in English | MEDLINE | ID: mdl-18620021

ABSTRACT

Nicotinic cholinergic receptors play a role in cardiovascular regulation in the lower brain stem. Herein, we present evidence that l-3,4-dihydroxyphenylalanine (DOPA), a putative neurotransmitter in the central nervous system, is involved in the depressor response to microinjection of nicotine into the nucleus tractus solitarii (NTS). Microinjection of nicotine into the medial area of the NTS led to decreases in arterial blood pressure and heart rate in anesthetized rats. Mecamylamine, a nicotinic receptor antagonist, microinjected into NTS, blocked the depressor and bradycardic responses to nicotine. Nicotine-induced depressor and bradycardic responses were blocked by DOPA cyclohexyl ester (DOPA CHE), an antagonist for DOPA. DOPA CHE did not modify the action of carbachol on excitatory postsynaptic potential in rat cortical slices. These results suggest that endogenous DOPA is involved in nicotine-induced depressor responses in the NTS of anesthetized rats.


Subject(s)
Cardiovascular System/drug effects , Levodopa/analogs & derivatives , Levodopa/antagonists & inhibitors , Nicotine/pharmacology , Nicotinic Agonists/pharmacology , Solitary Nucleus/drug effects , Animals , Blood Pressure/drug effects , Carbachol/pharmacology , Cholinergic Agonists/pharmacology , Excitatory Postsynaptic Potentials/drug effects , Heart Rate/drug effects , In Vitro Techniques , Levodopa/pharmacology , Male , Mecamylamine/pharmacology , Microinjections , Nicotinic Antagonists/pharmacology , Rats , Rats, Wistar
4.
Neuropharmacology ; 50(8): 909-16, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16504219

ABSTRACT

L-3,4-dihydroxyphenylalanine (DOPA) is a neurotransmitter candidate. To map the DOPAergic system functionally, DOPA-induced c-Fos expression was detected under inhibition of central aromatic L-amino acid decarboxylase (AADC). In rats treated with a central AADC inhibitor, DOPA significantly increased the number of c-Fos-positive nuclei in the paraventricular nuclei (PVN) and the nucleus tractus solitarii (NTS), and showed a tendency to increase in the supraoptic nuclei (SON), but not in the striatum. On the other hand, DOPA with a peripheral AADC inhibitor elevated the level of c-Fos-positive nuclei in the four regions, suggesting that DOPA itself induces c-Fos expression in the SON, PVN and NTS. In rats treated with 6-hydroxydopamine (6-OHDA) to lesion the nigrostriatal dopamine (DA) pathway, DOPA significantly induced c-Fos expression in the four regions under the inhibition of peripheral AADC. However, under the inhibition of central AADC, DOPA did not significantly increase the number of c-Fos-positive nuclei in the four regions, suggesting that DOPA at least in part induces c-Fos expression through its conversion to DA. It was likely that the 6-OHDA lesion enhanced the response to DA, but attenuated that to DOPA itself. In conclusion, we proposed that the SON, PVN and NTS include target sites for DOPA itself.


Subject(s)
Aromatic-L-Amino-Acid Decarboxylases/metabolism , Central Nervous System/drug effects , Dopamine Agents/pharmacology , Gene Expression Regulation/drug effects , Levodopa/pharmacology , Proto-Oncogene Proteins c-fos/metabolism , Animals , Benserazide/pharmacology , Cell Count/methods , Drug Interactions , Enzyme Inhibitors/pharmacology , Functional Laterality , Hydrazines/pharmacology , Immunohistochemistry/methods , Male , Medial Forebrain Bundle/injuries , Motor Activity/drug effects , Oxidopamine/toxicity , Rats , Rats, Sprague-Dawley
5.
Br J Anaesth ; 95(6): 803-10, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16227338

ABSTRACT

BACKGROUND: Ketamine has been reported to exert anti-inflammatory effects on macrophages stimulated with lipopolysaccharide (LPS) in vitro and in vivo. Several studies have reported conflicting results regarding the effects of propofol on cytokine production from immune cells. However, there have been no reports of the effects of these agents on inflammatory responses in glial cells. We investigated the effects of ketamine and propofol on LPS-induced production of nitric oxide, tumour necrosis factor-alpha (TNF-alpha) and prostaglandin E(2) (PGE(2)) from primary cultures of rat glial cells in vitro. METHODS: Glial cells were stimulated with LPS in the absence and presence of various concentrations of ketamine (30-1000 microM) or propofol (30 and 300 microM). Nitric oxide released into the culture media was determined by measuring nitrite using the Griess reaction, and concentrations of TNF-alpha and PGE(2) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Ketamine reduced LPS-induced TNF-alpha production without significant inhibition of nitrite release in mixed glial cells, astrocyte cultures and microglial cultures. Ketamine also inhibited LPS-induced production of PGE(2) in astrocyte cultures. In contrast, propofol had no effect on LPS-induced nitrite or TNF-alpha production in mixed glial cells. CONCLUSIONS: The data demonstrate that ketamine inhibited some of the inflammatory responses of both astrocytes and microglial cells treated with LPS without causing major change in nitric oxide release. Propofol had no effect on the production of nitric oxide or TNF-alpha from LPS-stimulated glial cells.


Subject(s)
Inflammation Mediators/metabolism , Ketamine/pharmacology , Lipopolysaccharides/pharmacology , Neuroglia/drug effects , Propofol/pharmacology , Anesthetics, Dissociative/pharmacology , Anesthetics, Intravenous/pharmacology , Animals , Astrocytes/drug effects , Astrocytes/metabolism , Cells, Cultured , Dinoprostone/biosynthesis , Dose-Response Relationship, Drug , Lipopolysaccharides/antagonists & inhibitors , Microglia/drug effects , Microglia/metabolism , Neuroglia/metabolism , Nitric Oxide/biosynthesis , Rats , Rats, Wistar , Tumor Necrosis Factor-alpha/biosynthesis
6.
Eur Respir J ; 23(5): 665-70, 2004 May.
Article in English | MEDLINE | ID: mdl-15176677

ABSTRACT

p27Kip1 is a cyclin-dependent kinase inhibitor, it negatively regulates G1 progression and is reported to modulate apoptosis. Phosphorylation of this protein is thought to regulate its intracellular localisation and affect its stability. The aim of this study was to regulate p27Kip1 expression levels, and to examine how this protein affects cell cycle status and modulates viability in A549 lung adenocarcinoma cells. In addition, the association between phosphorylation status of p27Kip1 and its intracellular localisation was investigated, using expression vectors with cDNA of p27Kip1 or mutants in which the phosphorylation sites had been mutated. Although overexpression of p27Kip1 reduced cell cycle progression, its removal did not change cell cycle status. Modest induction of p27Kip1 rescued adenovector-induced apoptosis and its removal with short interfering RNA increased spontaneous cell death. It was also observed that p27Kip1 localised mainly in the cytoplasm, and forced expression of p27Kip1 cDNA with the substitution of serine (S) 10, threonine (T) 157 and T198 to glutamate (phosphor-mimetic) induced its cytoplasmic localisation. In conclusion, p27Kip1, when expressed physiologically, exists mainly in the cytoplasm, has little effect on cell cycle status and contributes viability in A549 lung adenocarcinoma cells. It was also surmised that intracellular localisation of p27Kip1 dominates its function and that its localisation was partly determined by its phosphorylation.


Subject(s)
Adenocarcinoma/pathology , Adenocarcinoma/physiopathology , Cell Cycle Proteins/metabolism , Cell Cycle , Enzyme Inhibitors/metabolism , Lung Neoplasms/pathology , Lung Neoplasms/physiopathology , Tumor Suppressor Proteins/metabolism , Amino Acid Substitution , Apoptosis , Cell Cycle Proteins/antagonists & inhibitors , Cell Cycle Proteins/genetics , Cell Line , Cell Survival , Cyclin-Dependent Kinase Inhibitor p27 , Cytoplasm/metabolism , Glutamic Acid , Humans , Phosphorylation , Serine , Threonine , Tissue Distribution , Tumor Suppressor Proteins/antagonists & inhibitors , Tumor Suppressor Proteins/genetics
7.
Eur J Pharmacol ; 424(3): 199-202, 2001 Jul 27.
Article in English | MEDLINE | ID: mdl-11672563

ABSTRACT

We investigated whether stress induces the release of L-3,4-dihydroxyphenylalanine (DOPA) and dopamine from the nucleus accumbens in conscious rats and characterized the stress-induced response. Electrical foot-shock stress induced both DOPA and dopamine release, measured by microdialysis, from the nucleus accumbens in freely moving rats. Pretreatment of rats with mecamylamine completely blocked stress-induced DOPA release, but only partially blocked dopamine release. Diazepam did not affect the foot-shock-induced release of DOPA, while the same dose of diazepam partially blocked the stress-induced release of dopamine. These findings suggest a tonic function of central nicotinic receptors in stress-induced DOPA release from the nucleus accumbens in conscious rats.


Subject(s)
Levodopa/metabolism , Nucleus Accumbens/metabolism , Receptors, Nicotinic/physiology , Stress, Physiological/physiopathology , Animals , Anticonvulsants/pharmacology , Behavior, Animal/drug effects , Diazepam/pharmacology , Dopamine/metabolism , Electroshock , Foot , Male , Mecamylamine/pharmacology , Microdialysis , Motor Activity/drug effects , Movement , Nicotinic Antagonists/pharmacology , Nucleus Accumbens/drug effects , Rats , Rats, Sprague-Dawley , Receptors, Nicotinic/drug effects
8.
Neuroscience ; 104(1): 1-14, 2001.
Article in English | MEDLINE | ID: mdl-11311526

ABSTRACT

We previously proposed that L-3,4-dihydroxyphenylalanine (L-DOPA) is a neurotransmitter in the CNS. Receptor and transporter molecules for L-DOPA, however, have not been determined. In the present study, in order to localize the uptake sites of L-DOPA in the CNS, we performed autoradiographic uptake studies using L-[14C]DOPA and L-[3H]DOPA in the uptake study on rat brain slice preparations, and further analyzed the properties of L-DOPA uptake. Image analysis of the L-[14C]DOPA autoradiogram showed a unique heterogeneous distribution of uptake sites in the brain. The intensity was relatively high in the cerebral cortex, the hypothalamus, the cerebellum and the hippocampus, while the density was moderate or even low in the striatum and the substantia nigra. L-DOPA and phenylalanine, but not dopamine (10mM) were able to almost completely inhibit the uptake of L-[14C]DOPA to basal levels. Microautoradiographic studies using L-[3H]DOPA revealed accumulation of dense grains in the median eminence, the supraoptic nucleus of the hypothalamus, the cerebral cortex (layer I) and the hippocampus. In the cerebellum, grains formed in clusters surrounding the Purkinje cells. This grain accumulation was concluded to be in Bergmann glial cells, since the morphological pattern of grain accumulation was similar to that of the immunoreactivity of the glutamate aspartate transporter, a marker protein for Bergmann glial cells. In the hippocampus, the grain density significantly decreased under Na(+)-free conditions. In addition, grain density also decreased in the absence of Cl(-). In contrast, grains in the choroid plexus and the ependymal cell layer, were not affected by the absence of Na(+). These findings indicated that the uptake of L-DOPA occurs via various types of large neutral amino acid transport mechanisms. It appears that neuronal and/or glial cells, which take up L-DOPA in a Na(+)-dependent manner, exist in the CNS. Our finding further supports the concept that L-DOPA itself may act as a neurotransmitter or neuromodulator.


Subject(s)
Central Nervous System/metabolism , Levodopa/metabolism , Neurons/metabolism , Neurotransmitter Agents/metabolism , Sodium/metabolism , Animals , Autoradiography , Carbon Radioisotopes/pharmacokinetics , Central Nervous System/cytology , Central Nervous System/drug effects , Immunohistochemistry , Levodopa/pharmacokinetics , Male , Neuroglia/drug effects , Neuroglia/metabolism , Neurons/drug effects , Neurotransmitter Agents/pharmacokinetics , Rats , Rats, Wistar , Tritium/pharmacokinetics
9.
Brain Res Bull ; 54(4): 413-9, 2001 Mar 01.
Article in English | MEDLINE | ID: mdl-11306194

ABSTRACT

Effects of excitatory amino acid receptor antagonists and voltage-dependent Ca(2+) channel antagonists on ischemia-induced intracellular free Ca(2+) accumulation in rat hippocampal slices were examined. Ischemia caused a large Ca(2+) accumulation in CA1 region but a small Ca(2+) accumulation in CA3 and dentate gyrus regions. When applied during ischemia, the NMDA receptor antagonist MK-801 ((+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine maleate) inhibited the ischemic Ca(2+) accumulation only in the CA1, but the non-NMDA receptor antagonist CNQX (6-cyano-7-nitroquinoxaline-2,3-dione) inhibited it in all the three regions. The L-type Ca(2+) channel antagonists nifedipine and verapamil inhibited the ischemic Ca(2+) accumulation only in the CA1 region, but omega-conotoxin, a N- and L-type Ca(2+) channel antagonist inhibited the Ca(2+) accumulation in all the three regions of the hippocampus. When applied after 5-min ischemia, nifedipine but not MK-801, inhibited sustained postiscehmic Ca(2+) elevation in the CA1 region but not in the CA3 and dentate gyrus regions. These findings suggest that the enhanced ischemia-induced Ca(2+) accumulation in the CA1 region is mediated via activation of both NMDA receptors and L-type-like Ca(2+) channels. It appears that sustained postischemic Ca(2+) elevation in the CA1 region is mediated via activation of L-type-like Ca(2+) channels, but not of NMDA receptors.


Subject(s)
Calcium Channel Blockers/pharmacology , Calcium/metabolism , Excitatory Amino Acid Antagonists/pharmacology , Hippocampus/drug effects , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Animals , Cell Hypoxia/drug effects , Cell Hypoxia/physiology , Dizocilpine Maleate/pharmacology , Glutamic Acid/pharmacology , Hippocampus/metabolism , Ischemia/metabolism , Male , Nifedipine/pharmacology , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate/metabolism
10.
Nat Neurosci ; 4(4): 367-73, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11276226

ABSTRACT

Semaphorin 3A is a chemorepulsive axonal guidance molecule that depolymerizes the actin cytoskeleton and collapses growth cones of dorsal root ganglia neurons. Here we investigate the role of LIM-kinase 1, which phosphorylates an actin-depolymerizing protein, cofilin, in semaphorin 3A-induced growth cone collapse. Semaphorin 3A induced phosphorylation and dephosphorylation of cofilin at growth cones sequentially. A synthetic cell-permeable peptide containing a cofilin phosphorylation site inhibited LIM-kinase in vitro and in vivo, and essentially suppressed semaphorin 3A-induced growth cone collapse. A dominant-negative LIM kinase, which could not be activated by PAK or ROCK, suppressed the collapsing activity of semaphorin 3A. Phosphorylation of cofilin by LIM-kinase may be a critical signaling event in growth cone collapse by semaphorin 3A.


Subject(s)
Actins/metabolism , Glycoproteins/metabolism , Growth Cones/physiology , Microfilament Proteins/metabolism , Neurons, Afferent/enzymology , Protein Kinases/metabolism , Actin Depolymerizing Factors , Amino Acid Sequence , Animals , Blotting, Western , Cells, Cultured , Ganglia, Spinal/cytology , Genetic Vectors/genetics , Growth Cones/ultrastructure , Humans , Lim Kinases , Mice , Microfilament Proteins/genetics , Microscopy, Fluorescence , Molecular Sequence Data , Nerve Growth Factors/metabolism , Neurons, Afferent/cytology , Peptides/metabolism , Phosphorylation , Protein Kinases/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Semaphorin-3A , Transfection
11.
J Neurochem ; 76(3): 815-24, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11158253

ABSTRACT

Glutamate is implicated in neuronal cell death. Exogenously applied DOPA by itself releases neuronal glutamate and causes neuronal cell death in in vitro striatal systems. Herein, we attempt to clarify whether endogenous DOPA is released by 10 min transient ischemia due to four-vessel occlusion during rat striatal microdialysis and, further, whether DOPA, when released, functions to cause glutamate release and resultant delayed neuronal cell death. Ischemia increased extracellular DOPA, dopamine, and glutamate, and elicited neuronal cell death 96 h after ischemic insult. Inhibition of striatal L-aromatic amino acid decarboxylase 10 min before ischemia increased markedly basal DOPA, tripled glutamate release with a tendency of decrease in dopamine release by ischemia, and exaggerated neuronal cell death. Intrastriatal perfusion of 10-30 nM DOPA cyclohexyl ester, a competitive DOPA antagonist, 10 min before ischemia, concentration-dependently decreased glutamate release without modification of dopamine release by ischemia. At 100 nM, the antagonist elicited a slight ceiling effect on decreases in glutamate release by ischemia and protected neurons from cell death. Glutamate was released concentration-dependently by intrastriatal perfusion of 0.3-1 mM DOPA and stereoselectively by 0.6 mM DOPA. The antagonist elicited no hypothermia during and after ischemia. Endogenously released DOPA is an upstream causal factor for glutamate release and resultant delayed neuronal cell death by brain ischemia in rat striata. DOPA antagonist has a neuroprotective action.


Subject(s)
Corpus Striatum/metabolism , Dihydroxyphenylalanine/physiology , Glutamic Acid/metabolism , Ischemic Attack, Transient/metabolism , Levodopa/analogs & derivatives , Neurons/physiology , Animals , Cell Death , Corpus Striatum/pathology , Dihydroxyphenylalanine/pharmacology , Dopamine/metabolism , Enzyme Inhibitors/pharmacology , Hydrazines/pharmacology , Levodopa/pharmacology , Male , Rats , Rats, Wistar , Time Factors
12.
Neurosci Lett ; 299(3): 213-6, 2001 Feb 23.
Article in English | MEDLINE | ID: mdl-11165773

ABSTRACT

In rat striata, DOPA released is a causal factor for glutamate release and resultant delayed neuron death by four-vessel occlusion. Nanomolar DOPA cyclohexyl ester (CHE), a potent and relatively stable competitive DOPA antagonist, protects these events. We tried to clarify whether DOPA CHE protects these events in hippocampal CA1 pyramidal cell layers most vulnerable against ischemia. Five to 10 min ischemia caused slight to mild glutamate release in 10 min samples during microdialysis and mild to severe neuron death 96 h after reperfusion. DOPA and dopamine were under assay limit in this design, but were basally detected by 20 min sampling and released by 20 min ischemia. In 10 min samples, intrahippocampal perfusion of 100 nM DOPA CHE 10 min before ischemia for 70 min did not inhibit glutamate release by 10 min ischemia, while it abolished glutamate release and protected delayed neuron death by 5 min ischemia. DOPA CHE is neuroprotective under a mild ischemic condition in rat hippocampus CA1.


Subject(s)
Brain Ischemia/drug therapy , Dihydroxyphenylalanine/metabolism , Glutamic Acid/metabolism , Hippocampus/drug effects , Levodopa/pharmacology , Nerve Degeneration/drug therapy , Neuroprotective Agents/pharmacology , Animals , Brain Ischemia/pathology , Brain Ischemia/physiopathology , Cell Death/drug effects , Cell Death/physiology , Dihydroxyphenylalanine/analogs & derivatives , Dihydroxyphenylalanine/antagonists & inhibitors , Hippocampus/pathology , Hippocampus/physiopathology , Levodopa/analogs & derivatives , Male , Nerve Degeneration/metabolism , Nerve Degeneration/physiopathology , Neurons/drug effects , Neurons/metabolism , Neurons/pathology , Organ Culture Techniques , Rats , Rats, Wistar , Reperfusion Injury/drug therapy , Reperfusion Injury/metabolism , Reperfusion Injury/physiopathology
13.
Mech Dev ; 97(1-2): 35-45, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11025205

ABSTRACT

Semaphorin III/collapsin-1 (Sema3A) guides a specific subset of neuronal growth cones as a repulsive molecule. In this study, we have investigated a possible role of non-neuronal Sema3A in lung morphogenesis. Expression of mRNAs of Sema3A and neuropilin-1 (NP-1), a Sema3A receptor, was detected in fetal and adult lungs. Sema3A-immunoreactive cells were found in airway and alveolar epithelial cells of the fetal and adult lungs. Immunoreactivity for NP-1 was seen in fetal and adult alveolar epithelial cells as well as endothelial cells. Immunoreactivity of collapsin response mediator protein CRMP (CRMP-2), an intracellular protein mediating Sema3A signaling, was localized in alveolar epithelial cells, nerve tissue and airway neuroendocrine cells. The expression of CRMP-2 increased during the fetal, neonate and adult periods, and this pattern paralleled that of NP-1. In a two-day culture of lung explants from fetal mouse lung (E11.5), with exogenous Sema3A at a dose comparable to that which induces growth cone collapse of dorsal root ganglia neurons, the number of terminal buds was reduced in a dose-dependent manner when compared with control or untreated lung explants. This decrease was not accompanied with any alteration of the bromodeoxyuridine-positive DNA-synthesizing fraction. A soluble NP-1 lacking the transmembrane and intracellular region, neutralized the inhibitory effect of Sema3A. The fetal lung explants from neuropilin-1 homozygous null mice grew normally in vitro regardless of Sema3A treatment. These results provide evidence that Sema3A inhibits branching morphogenesis in lung bud organ cultures via NP-1 as a receptor or a component of a possible multimeric Sema3A receptor complex.


Subject(s)
Glycoproteins/physiology , Lung/embryology , Nerve Tissue Proteins/physiology , Animals , Axons/physiology , Blotting, Western/methods , Female , Glycoproteins/genetics , Lung/pathology , Male , Mice , Mice, Inbred ICR , Morphogenesis , Nerve Tissue Proteins/genetics , Neuropilin-1 , Reverse Transcriptase Polymerase Chain Reaction , Semaphorin-3A
14.
Jpn J Pharmacol ; 82(1): 40-7, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10874587

ABSTRACT

We explored L-DOPA esters with chemically bulky structures to find a potent stable competitive antagonist against L-DOPA, compared to DOPA methyl ester (DOPA ME). In anesthetized rats, DOPA cyclohexyl ester (DOPA CHE), DOPA cyclopentyl ester (DOPA CPE) and DOPA cyclopentyldimethyl ester (DOPA CPDME) at 1 microgram microinjected into depressor sites of the nucleus tractus solitarii elicited or tended to elicit more marked antagonism against depressor responses to 60 ng L-DOPA, compared to DOPA ME. At 100 ng, DOPA CHE elicited the most potent antagonism. At 1 microgram, duration of the antagonistic activity of DOPA CHE was approximately three times longer than that of DOPA ME. During microdialysis of the nucleus accumbens, conversion from DOPA CHE at 1 microM perfused via probes to extracellular L-DOPA was the lowest among these compounds and less than one half of that from DOPA ME. Binding studies showed that the recognition site for L-DOPA differs from ionotropic glutamatergic, dopaminergic D1 and D2 receptors. We recently found that L-DOPA evoked by transient ischemia may act as a DOPA CHE-sensitive causal factor for glutamate release and resultant neuronal cell death. DOPA CHE is the most potent, relatively stable competitive antagonist against L-DOPA and is a useful mother compound to develop neuroprotective drugs.


Subject(s)
Levodopa/antagonists & inhibitors , Neuroprotective Agents/pharmacology , Animals , Binding, Competitive , Blood Pressure/drug effects , Glutamic Acid/metabolism , Heart Rate/drug effects , Male , Microdialysis , Microinjections , Rats , Rats, Sprague-Dawley , Rats, Wistar
15.
Jpn J Pharmacol ; 82(4): 273-9, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10875745

ABSTRACT

The semaphorin family comprises secreted and transmembrane signaling proteins that function in the nervous, immune, respiratory and cardiovascular systems. Sema3A, a secreted type of semaphorin, is now recognized as the most potent repulsive molecule inhibiting or repelling neurite outgrowth. The biological actions of Sema3A are mediated via neuropilin (Npn)-1, a receptor or one of the components of a receptor complex for Sema3A. Although the molecular mechanisms of Sema3A-Npn-1 signaling are largely unknown, a pertussis toxin-sensitive trimeric G protein(s), Rac-1, collapsin response mediator protein (CRMP), cyclic nucleotides and tyrosine kinase(s) have been implicated as essential and/or modulatory components of these processes. As repulsive molecules could be impediments to axon outgrowth, determining how these repulsive molecules exert their actions has the potential of uncovering new therapeutic approaches to injury and/or degeneration of neuronal tissues.


Subject(s)
Axons/physiology , Nerve Growth Factors/physiology , Nerve Regeneration/physiology , Nerve Tissue Proteins/physiology , Semaphorins , Animals , Cell Adhesion Molecules, Neuronal/physiology , Humans , Intercellular Signaling Peptides and Proteins , Neuropilin-1 , Semaphorin-3A
16.
J Biol Chem ; 275(31): 23973-80, 2000 Aug 04.
Article in English | MEDLINE | ID: mdl-10818093

ABSTRACT

We previously identified Rho-associated protein kinase (Rho-kinase) as a specific effector of Rho. In this study, we identified collapsin response mediator protein-2 (CRMP-2), as a novel Rho-kinase substrate in the brain. CRMP-2 is a neuronal protein whose expression is up-regulated during development. Rho-kinase phosphorylated CRMP-2 at Thr-555 in vitro. We produced an antibody that specifically recognizes CRMP-2 phosphorylated at Thr-555. Using this antibody, we found that Rho-kinase phosphorylated CRMP-2 downstream of Rho in COS7 cells. Phosphorylation of CRMP-2 was observed in chick dorsal root ganglion neurons during lysophosphatidic acid (LPA)-induced growth cone collapse, whereas the phosphorylation was not detected during semaphorin-3A-induced growth cone collapse. Both LPA-induced CRMP-2 phosphorylation and LPA-induced growth cone collapse were inhibited by Rho-kinase inhibitor HA1077 or Y-32885. LPA-induced growth cone collapse was also blocked by a dominant negative form of Rho-kinase. On the other hand, semaphorin-3A-induced growth cone collapse was not inhibited by a dominant negative form of Rho-kinase. Furthermore, overexpression of a mutant CRMP-2 in which Thr-555 was replaced by Ala significantly inhibited LPA-induced growth cone collapse. These results demonstrate the existence of Rho-kinase-dependent and -independent pathways for growth cone collapse and suggest that CRMP-2 phosphorylation by Rho-kinase is involved in the former pathway.


Subject(s)
Nerve Tissue Proteins/metabolism , Neurons/physiology , Phosphoproteins/metabolism , Protein Serine-Threonine Kinases/metabolism , Amino Acid Sequence , Animals , Antibodies , Brain Chemistry , COS Cells , Cattle , Ganglia, Spinal/cytology , Humans , Intercellular Signaling Peptides and Proteins , Intracellular Signaling Peptides and Proteins , Lysophospholipids/pharmacology , Molecular Sequence Data , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/immunology , Nerve Tissue Proteins/pharmacology , Neurons/drug effects , Phosphoproteins/immunology , Phosphorylation , Recombinant Proteins/metabolism , Semaphorin-3A , Substrate Specificity , rho-Associated Kinases
17.
Biochim Biophys Acta ; 1466(1-2): 61-70, 2000 Jun 01.
Article in English | MEDLINE | ID: mdl-10825431

ABSTRACT

Although L-3,4-dihydroxyphenylalanine (L-DOPA) is claimed to be a neurotransmitter in the central nervous system (CNS), receptor or transporter molecules for L-DOPA have not been determined. In an attempt to identify a transporter for L-DOPA, we examined whether or not an active and high affinity L-DOPA transport system is expressed in Xenopus laevis oocytes injected with poly A(+) RNA prepared from several tissues. Among the poly A(+) RNAs tested, rabbit intestinal epithelium poly A(+) RNA gave the highest transport activity for L-[(14)C]DOPA in the oocytes. The uptake was approximately five times higher than that of water-injected oocytes, and was partially Na(+)-dependent. L-Tyrosine, L-phenylalanine, L-leucine and L-lysine inhibited this transport activity, whereas D-DOPA, dopamine, glutamate and L-DOPA cyclohexylester, an L-DOPA antagonist did not affect this transport. Coinjection of an antisense cRNA, as well as oligonucleotide complementary to rabbit rBAT (NBAT) cDNA almost completely inhibited the uptake of L-[(14)C]DOPA in the oocytes. On the other hand, an antisense cRNA of rabbit 4F2hc barely affected this L-[(14)C]DOPA uptake activity. rBAT was thus responsible for the L-[(14)C]DOPA uptake activity expressed in X. laevis oocytes injected with poly A(+) RNA from rabbit intestinal epithelium. As rBAT is localized at the target regions of L-DOPA in the CNS, rBAT might be one of the components involved in L-DOPAergic neurotransmission.


Subject(s)
Amino Acid Transport Systems, Basic , Amino Acids/metabolism , Carrier Proteins/metabolism , Levodopa/pharmacokinetics , Membrane Glycoproteins/metabolism , Neurotransmitter Agents/pharmacokinetics , RNA, Messenger/metabolism , Animals , Biological Transport , Carrier Proteins/genetics , Female , Gene Expression , Intestinal Mucosa/metabolism , Intestine, Small/metabolism , Ions , Kinetics , Male , Membrane Glycoproteins/genetics , Microinjections/methods , Oocytes/metabolism , RNA, Messenger/administration & dosage , Rabbits , Rats , Rats, Wistar , Sodium/metabolism , Xenopus laevis
18.
J Cell Biol ; 149(2): 411-22, 2000 Apr 17.
Article in English | MEDLINE | ID: mdl-10769032

ABSTRACT

Axonal growth cone collapse is accompanied by a reduction in filopodial F-actin. We demonstrate here that semaphorin 3A (Sema3A) induces a coordinated rearrangement of Sema3A receptors and F-actin during growth cone collapse. Differential interference contrast microscopy reveals that some sites of Sema3A-induced F-actin reorganization correlate with discrete vacuoles, structures involved in endocytosis. Endocytosis of FITC-dextran by the growth cone is enhanced during Sema3A treatment, and sites of dextran accumulation colocalize with actin-rich vacuoles and ridges of membrane. Furthermore, the Sema3A receptor proteins, neuropilin-1 and plexin, and the Sema3A signaling molecule, rac1, also reorganize to vacuoles and membrane ridges after Sema3A treatment. These data support a model whereby Sema3A stimulates endocytosis by focal and coordinated rearrangement of receptor and cytoskeletal elements. Dextran accumulation is also increased in retinal ganglion cell (RGC) growth cones, in response to ephrin A5, and in RGC and DRG growth cones, in response to myelin and phorbol-ester. Therefore, enhanced endocytosis may be a general principle of physiologic growth cone collapse. We suggest that growth cone collapse is mediated by both actin filament rearrangements and alterations in membrane dynamics.


Subject(s)
Actins/physiology , Glycoproteins/pharmacology , Nerve Growth Factors/pharmacology , Nerve Tissue Proteins/physiology , Neurons/cytology , Neurons/physiology , Actins/drug effects , Animals , Cell Adhesion Molecules/physiology , Cell Membrane/drug effects , Cell Membrane/physiology , Cell Membrane/ultrastructure , Chick Embryo , Endocytosis/drug effects , Endocytosis/physiology , Ganglia, Spinal/cytology , Microscopy, Interference/methods , Nerve Tissue Proteins/drug effects , Neurons/ultrastructure , Neuropilin-1 , Receptors, Cell Surface/physiology , Retina/embryology , Semaphorin-3A , Signal Transduction , rac1 GTP-Binding Protein/physiology
19.
Gene ; 261(2): 259-67, 2000 Dec 31.
Article in English | MEDLINE | ID: mdl-11167013

ABSTRACT

The vertebrate CRMP (collapsin-response-mediator protein) gene family comprises at least four members. These CRMPs exhibit about 60% amino acid identity with vertebrate dihydropyrimidinase (DHP), an amidohydrolase involved in the pyrimidine degradation pathway. CRMP is also referred to as DRP (DHP-related protein), TOAD-64 (turned on after division, 64 kDa) and Ulip (Unc-33-like phosphoprotein). These vertebrate CRMPs are expressed mainly in early neuronal differentiation, which suggests that they play a role in neuronal development. In this study we isolated two cDNA clones from nematode C. elegans based on their sequence homology to vertebrate CRMPs and DHP. These two molecules, termed CeCRMP/DHP-1 and -2, turned out to be Ulip-B and -A, respectively, which were previously identified in the C. elegans genomic database by Byk et al. (1998). These newly isolated molecules were believed to represent a common ancestral state before the gene duplication between CRMPs and DHP. CeCRMP/DHP-1 and -2 protein retained all putative zinc-binding residues thought to be essential for the amidohydrolase activity of DHP and exhibited a weak amidohydrolase activity when 5-bromo-dihydrouracil was used as a substrate. Whole-mount in situ hybridization and expression analysis using GFP fusions revealed that CeCRMP/DHP-1 was transiently expressed in the hypodermis of C. elegans during the early larva stage. CeCRMP/DHP-1 was also expressed in a single nerve cell between the pharynx and ring neuropil. On the other hand, expression of CeCRMP/DHP-2 was observed in the body wall muscle throughout the lifespan of C. elegans. These results indicate that a major site of CeCRMP/DHP-1 and -2 expression is non-neuronal. Targeted gene disruption of CeCRMP/DHP-2 caused no particular difference in appearance or movement phenotype.


Subject(s)
Caenorhabditis elegans/genetics , Nerve Tissue Proteins/genetics , Phosphoproteins/genetics , Amidohydrolases/genetics , Amidohydrolases/metabolism , Amino Acid Sequence , Animals , COS Cells , Caenorhabditis elegans/embryology , Caenorhabditis elegans/growth & development , Cloning, Molecular , DNA, Complementary/chemistry , DNA, Complementary/genetics , Embryo, Nonmammalian/metabolism , Gene Expression Regulation, Developmental , Green Fluorescent Proteins , In Situ Hybridization , Intercellular Signaling Peptides and Proteins , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Molecular Sequence Data , Mutation , Nerve Tissue Proteins/metabolism , Phosphoproteins/metabolism , Phylogeny , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology, Amino Acid
20.
J Neurobiol ; 41(3): 326-39, 1999 Nov 15.
Article in English | MEDLINE | ID: mdl-10526312

ABSTRACT

During axonal growth, repulsive guidance cues cause growth cone collapse and retraction. In the chick embryo, membranes from the posterior part of the optic tectum containing ephrins are original collapsing factors for axons growing from the temporal retina. We investigated signal transduction pathways in retinal axons underlying this membrane-evoked collapse. Perturbation experiments using pertussis toxin (PTX) showed that membrane-induced collapse is mediated via G(o/i) proteins, as is the case for semaphorin/collapsin-1-induced collapse. Studies with Indo-1 revealed that growth cone collapse by direct activation of G(o/i) proteins with mastoparan did not cause elevation of the intracellular Ca(2+) level, and thus this signal transduction pathway is Ca(2+) independent. Application of the protein phosphatase inhibitor okadaic acid alone induced growth cone collapse in retinal culture, suggesting signals involving protein dephosphorylation. In addition, pretreatment of retinal axons with olomoucine, a specific inhibitor of cdk5 (tau kinase II), prevented mastoparan-evoked collapse. Olomoucine also blocks caudal tectal membrane-mediated collapse. These results suggest that rearrangement of the cytoskeleton is mediated by tau phosphorylation. Immunostaining visualized complementary distributions of tau phospho- and dephosphoisoforms within the growth cone, which also supports the involvement of tau. Taking these findings together, we conclude that cdk5 and tau phosphorylation probably lie downstream of growth cone collapse signaling mediated by PTX-sensitive G proteins.


Subject(s)
Axons/chemistry , Cyclin-Dependent Kinases/metabolism , Growth Cones/chemistry , Growth Cones/physiology , Heterotrimeric GTP-Binding Proteins/physiology , Retina/embryology , tau Proteins/metabolism , Animals , Calcium/metabolism , Chick Embryo , Enzyme Inhibitors/pharmacology , Growth Cones/drug effects , Immunohistochemistry , Intercellular Signaling Peptides and Proteins , Okadaic Acid/pharmacology , Peptides , Pertussis Toxin , Retina/chemistry , Retina/cytology , Superior Colliculi/chemistry , Superior Colliculi/cytology , Superior Colliculi/embryology , Virulence Factors, Bordetella/pharmacology , Wasp Venoms/pharmacology
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