ABSTRACT
Yellow vein mosaic disease of mesta, a compatible plant virus interaction poses a serious threat to mesta cultivation in India. Plants respond to invasion by pathogens with multi component defense responses particularly in incompatible interaction. With the aim of understanding, a biochemical approach was attempted to study the cellular redox status in early stages of yellow vein mosaic virus infection associated with different age's plant of Hibiscus cannabinus. Comparative analysis of GSH and GSSG content in infected and control plant of different ages indicated that infected plants are under oxidative or nitrosative stress condition. A significant change was observed in Glutathione Reductase, Catalase and Ascorbate Peroxidase level in early stage of infection. We also showed microscopic evidence of nitrosylated thiols in infected leaves, stems and roots of H. cannabinus. Furthermore, we identified few defense related proteins in infected plant using MALDI TOF mass spectrometric analysis.
Subject(s)
Begomovirus/physiology , Hibiscus/metabolism , Hibiscus/virology , Sulfhydryl Compounds/metabolism , Ascorbate Peroxidases , Catalase/metabolism , Glutathione Reductase/metabolism , Host-Pathogen Interactions , Oxidative Stress , Peroxidases/metabolism , Plant Leaves/metabolism , Plant Leaves/virology , Plant Roots/metabolism , Plant Roots/virology , Plant Stems/metabolism , Plant Stems/virology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
Nitric oxide (NO) plays a key role in plant diseases resistance. Here we have first time demonstrated that begomovirus infection in susceptible H. cannabinus plants, results in elevated NO and reactive nitrogen species production during early infection stage not only in infected leaf but also in root and shoot. Production of NO was further confirmed by oxyhemoglobin assay. Furthermore, we used Phenyl alanine ammonia lyase as marker of pathogenesis related enzyme. In addition evidence for protein tyrosine nitration during the early stage of viral infection clearly showed the involvement of nitrosative stress.
