The Prevalence of Virulent and Multidrug-Resistant Enterococci in River Water and in Treated and Untreated Municipal and Hospital Wastewater.

The aim of this study is to describe the drug resistance and virulence of enterococci in river water sampled downstream (DRW) and upstream (URW) from the wastewater discharge point, to determine the pool of virulent and drug-resistant enterococci in untreated wastewater (UWW) and the extent to which these bacteria are eliminated from hospital wastewater (HWW) and municipal wastewater treated (TWW) by biological and mechanical methods in a wastewater treatment plant (WWTP). A total of 283 strains were identified with the use of culture-dependent methods and PCR, including seven different species including E. faecalis and E. faecium which were predominant in all analyzed samples. Majority of the strains were classified as multidrug resistant (MDR), mostly on streptomycin and trimethoprim. Strains isolated from wastewater and DRW harbored van genes conditioning phenotypic resistance to vancomycin, the highest percentage of vancomycin-resistant strains (57.0%), mostly strains harboring vanC1 genes (27.6%), was noted in TWW. More than 65.0% of the isolated strains had different virulence genes, the highest number of isolates were positive for cell wall adhesin efaA and sex pheromones cob, cpd, and ccf which participate in the induction of virulence. Many of the strains isolated from TWW were resistant to a higher number of drugs and were more virulent than those isolated from UWW and HWW. The enterococci isolated from DRW and wastewater were characterized by similar multidrug resistance and virulence profiles, and significant correlations were observed between these groups of isolates. These findings suggest that pathogenic enterococci are released with TWW and can spread in the river, pose a serious epidemiological threat and a risk to public health.

The impact of urban areas on the water quality gradient along a lowland river.

The effects of five towns on river water pollution were examined along the Lyna River (southern watershed of the Baltic Sea, northern Poland). The relationships among the spatially derived indicators of urbanization, environmental variables, and physico-chemical and microbiological data (heterotrophic plate count at 22 and 37 °C, and fecal coli) obtained from longitudinal river profiling have been examined with the use of multivariate analyses such as principal component analysis with factor analysis (PCA/FA) and hierarchical cluster analysis (HCA). We recognized the river channel as an environmental path that links serial urban areas into an "urban river continuum." An overall increasing trend in nutrients and indicator bacteria from suburban headwaters to urbanized sections of the river was detected despite a significant decrease in those between the towns. We concluded that the role of a multicity is equally as important as a single urban area in predicting the impacts of man-made pollutants on river water quality.

Ammonia Nitrogen Transformations in a Reactor with Aggregate made of Sewage Sludge Combustion Fly Ash.

The influence of light weight aggregate made of fly ash from sewage sludge thermal treatment (FASSTT LWA) on ammonia nitrogen metabolism, and on quantitative and qualitative changes of microorganisms colonizing the filling, was investigated. Two reactors were used in the experiment. The first was filled with gravel, the other with FASSTT LWA. The reactors were operated with a wastewater hydraulic loading rate of 5 mm(3) mm(-2) d(-1). During the eleven-week experiment, high efficiency of ammonia removal was observed. The lower concentrations of nitrites and nitrates in the effluent indicate that ammonia nitrogen removal resulted not just from nitrification. Nitrate concentration increase was reflected in a decrease in nitrogen removal efficiency. One possible explanation for this phenomenon is that in the period when ammonia nitrogen and nitrites were present in the reactor's FASSTT LWA filling, facilitating conditions occurred for the deammonification process.

Hydrogenotrophic denitrification process efficiency and the number of denitrifying bacteria (MPN) in the sequencing batch biofilm reactor (SBBR) with platinum and carbon anodes.

This work reports on the effect of electric current density and anode material (platinum, carbon) on the concentration of oxidized and mineral forms of nitrogen, on physical parameters (pH, redox potential, electrical conductivity) and the number of denitrifying bacteria in the biofilm (MPN). Experiments were conducted under anaerobic conditions without and with the flow of electric current (with density of 79 mA · m(-2) and 132 mA · m(-2)). Results obtained in the study enabled concluding that increasing density of electric current caused a decreasing concentration of nitrate in the reactor with platinum anode (R1) and carbon anode (R2). Its concentration depended on anode material. The highest hydrogenotrophic denitrification efficiency was achieved in R2 in which the process was aided by inorganic carbon (CO2) that originated from carbon anode oxidation and the electrical conductivity of wastewater increased as a result of the presence of HCO3(-) and CO3(2-) ions. Strong oxidizing properties of the platinum anode (R1) prevented the accumulation of adverse forms of nitrogen, including nitrite and ammonia. The increase in electric current density affected also a lower number of denitrifying bacteria (MPN) in the biofilm in both reactors (R1 and R2). Metal oxides accumulated on the surface of the cathode had a toxic effect upon microorganisms and impaired the production of a hydrogen donor.

Evaluation of the distribution of fecal indicator bacteria in a river system depending on different types of land use in the southern watershed of the Baltic Sea.

The aim of the study was to determine the effects of land use management on changes in the fecal contamination of water in the Lyna River, one of the main lowland watercourses in the southern watershed of the Baltic Sea (northern Poland). A total of 120 water samples were collected in different seasons of 2011 and 2012 at 15 sites where the river intersected forest (FA), agricultural (AA), and urbanized (UA) areas. Fecal indicator bacteria (FIB), the counts of Enterobacteriaceae and Escherichia coli, total bacterial counts (TBCs), and domain Bacteria (EUB338) were determined by culture-dependent and culture-independent methods. Temperature, pH, chemical oxygen demand, dissolved oxygen, total dissolved solids, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, orthophosphate, and total phosphorus were also determined. The lowest bacterial counts were noted in water samples collected in FA, and the highest in samples collected in UA. Statistically significant differences were determined between bacterial populations across the analyzed land use types and in different sampling seasons. Significant correlations were also observed between the populations of FIB and physicochemical parameters. The results indicate that land use type influenced FIB concentrations in river water. The combined use of conventional and molecular methods improves the accuracy of fecal contamination analyses in river ecosystems.

Microbial evaluation of sandboxes located in urban area.

This paper presents the results of a study on the degree of bacteriological pollution of sandboxes situated in fenced and unfenced housing estates located in an urban area in Olsztyn, Poland. Heterotrophic plate counts (HPC22, HPC37), Enterobacteriaceae, Escherichia coli, Enterococcus spp., Staphylococcus spp. and Clostridium perfringens determined by cultivation and fluorescence in situ hybridization (FISH) methods were used as indicators of the sanitary state. Their maximum number in the sand samples reached values of up to 5.4×10(7), 2.6×10(6), 3.3×10(4), 2.1×10(3), 1.8×10(4), 1.9×10(1) and 1.2×10(4)CFU/g, respectively. It was found that values of culture-independent method were two-four orders greater than those obtained by the cultivation method. Among identified Enterobacteriaceae, Pantoea spp. and Enterobacter cloacae were the most numerous, whereas Escherichia cells were detected only occasionally. Pathogenic bacteria of the genus Salmonella sp. were isolated from sandboxes also when E. coli were absent. Bacteria from Staphylococcus genus were isolated irrespective of the site and time of sampling. Additionally, the presence of molds and yeasts was studied. Maximum counts of these microorganisms amounted to 1.0×10(5) and to 3.5×10(4)CFU/g. Aspergillus, Penicillium, Alternaria and Trichoderma genera were most numerous among molds, whereas Trichosporon was detected most frequently among yeasts. Sandboxes in the fenced housing estate and those located in the area which is not close to trees were less polluted than the sand collected from sandboxes in the unfenced housing estate. Potentially pathogenic bacteria of the genus Salmonella spp. were identified in analyzed sandboxes, also when Toxocara and E. coli were absent. It seems that assessing the contamination of children's play areas basing only on fecal bacteria counts and by monitoring number of parasites' eggs may be insufficient to evaluate microbial pollution of sandboxes and may not fully reflect their safety for children.