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1.
Biochem Biophys Res Commun ; 663: 154-162, 2023 06 30.
Article in English | MEDLINE | ID: mdl-37141667

ABSTRACT

The TFDP1 gene codes for the heterodimeric partner DP1 of the transcription factor E2F. E2F, principal target of the tumor suppressor pRB, plays central roles in cell proliferation by activating a group of growth-related genes. E2F also mediates tumor suppression by activating tumor suppressor genes such as ARF, an upstream activator of the tumor suppressor p53, when deregulated from pRB upon oncogenic changes. Among 8 E2F family members (E2F1∼E2F8), expression of activator E2Fs (E2F1∼E2F3a) is induced at the G1/S boundary of the cell cycle after growth stimulation by E2F itself. However, mechanisms regulating DP1 expression are not known. We show here that over-expression of E2F1 and forced inactivation of pRB, by adenovirus E1a, induced TFDP1 gene expression in human normal fibroblast HFFs, suggesting that the TFDP1 gene is a target of E2F. Serum stimulation of HFFs also induced TFDP1 gene expression, but with different kinetics from that of the CDC6 gene, a typical growth-related E2F target. Both over-expression of E2F1 and serum stimulation activated the TFDP1 promoter. We searched for E2F1-responsive regions by 5' and 3' deletion of the TFDP1 promoter and by introducing point mutations in putative E2F1-responsive elements. Promoter analysis identified several GC-rich elements, mutation of which reduced E2F1-responsiveness but not serum-responsiveness. ChIP assays showed that the GC-rich elements bound deregulated E2F1 but not physiological E2F1 induced by serum stimulation. These results suggest that the TFDP1 gene is a target of deregulated E2F. In addition, knockdown of DP1 expression by shRNA enhanced ARF gene expression, which is specifically induced by deregulated E2F activity, suggesting that activation of the TFDP1 gene by deregulated E2F may function as a failsafe feedback mechanism to suppress deregulated E2F and maintain normal cell growth in the event that DP1 expression is insufficient relative to that of its partner activator E2Fs. a maximum of 6 keywords: E2F, DP1, TFDP1 gene, pRB, gene expression.


Subject(s)
E2F1 Transcription Factor , Gene Expression Regulation , Humans , E2F Transcription Factors/genetics , E2F Transcription Factors/metabolism , E2F1 Transcription Factor/genetics , E2F1 Transcription Factor/metabolism , Genes, Tumor Suppressor , Cell Cycle Proteins/metabolism , E2F3 Transcription Factor/metabolism , Transcription Factor DP1/genetics , Transcription Factor DP1/metabolism
2.
Sci Rep ; 8(1): 8438, 2018 05 31.
Article in English | MEDLINE | ID: mdl-29855511

ABSTRACT

The transcription factor E2F plays crucial roles in cell proliferation and tumor suppression by activating growth-related genes and pro-apoptotic tumor suppressor genes, respectively. It is generally accepted that E2F binds to target sequences with its heterodimeric partner DP. Here we show that, while knockdown of DP1 expression inhibited ectopic E2F1- or adenovirus E1a-induced expression of the CDC6 gene and cell proliferation, knockdown of DP1 and DP2 expression did not affect ectopic E2F1- or E1a-induced expression of the tumor suppressor ARF gene, an upstream activator of the tumor suppressor p53, activation of p53 or apoptosis. These observations suggest that growth related and pro-apoptotic E2F targets are regulated by distinct molecular mechanisms and contradict the threshold model, which postulates that E2F activation of pro-apoptotic genes requires a higher total activity of activator E2Fs, above that necessary for E2F-dependent activation of growth-related genes.


Subject(s)
E2F1 Transcription Factor/metabolism , Transcription Factor DP1/metabolism , Tumor Suppressor Protein p53/metabolism , ADP-Ribosylation Factors/genetics , ADP-Ribosylation Factors/metabolism , Apoptosis , Cell Cycle Proteins/genetics , Cell Cycle Proteins/metabolism , Cell Line , DNA-Binding Proteins/antagonists & inhibitors , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Dimerization , E2F1 Transcription Factor/chemistry , E2F1 Transcription Factor/genetics , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Promoter Regions, Genetic , RNA Interference , RNA, Small Interfering/metabolism , Transcription Factor DP1/antagonists & inhibitors , Transcription Factor DP1/genetics , Transcription Factors/antagonists & inhibitors , Transcription Factors/genetics , Transcription Factors/metabolism
3.
Mol Biotechnol ; 56(1): 50-63, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23820979

ABSTRACT

Cucumber mosaic virus (CMV) is a tripartite, positive sense RNA virus causing infections and yield losses to many plant species. Here, we generated a construct containing inverted repeat of 1,793 bp fragment of defective CMV replicase gene derived from RNA2 of cucumber mosaic virus strain O (CMV-O). The replicase gene was modified by deleting a 9 bp region between nucleotides 1909-1918. This caused a deletion in the active centre motif of polymerases, producing defective translated product 9 nucleotides shorter than the full length protein. The RNAi construct containing inverted repeat of the defective gene was used to produce transgenic tobacco lines expressing CMV-derived double-stranded RNA via Agrobacterium-mediated transformation. Of the four transgenic lines inoculated with CMV-O or CMV-Y in vitro and ex vivo, three lines (T1, T4 and T5) showed immunity to both strains of CMV as no symptoms were detected, whereas one line (T7) exhibited high resistance with mild symptoms limited to inoculation portions. No virus could be detected in uninoculated new leaves of the transgenic lines after RT-PCR and Dot-immunobinding assay analyses. Small interfering RNAs present in transgenic lines before and after virus challenge indicates that the resistance was acquired through RNA silencing.


Subject(s)
Agrobacterium tumefaciens/genetics , Cucumovirus/enzymology , Nicotiana/virology , Plant Leaves/virology , RNA-Dependent RNA Polymerase/genetics , RNA-Dependent RNA Polymerase/metabolism , Viral Proteins/metabolism , Agrobacterium tumefaciens/metabolism , Cucumovirus/genetics , Genes, Viral , Inverted Repeat Sequences , Plant Leaves/genetics , Plants, Genetically Modified , RNA Interference , RNA, Double-Stranded/metabolism , Sequence Deletion , Nicotiana/genetics , Viral Proteins/genetics
4.
FEBS Lett ; 557(1-3): 169-73, 2004 Jan 16.
Article in English | MEDLINE | ID: mdl-14741362

ABSTRACT

Various mutant lysozymes were constructed by genetic modification and secreted in yeast expression system to evaluate the changes in the antigenicity of hen egg lysozyme (HEL). Although Arg68, the most critical residue to antigenicity of HEL, was substituted with Gln, the binding of monoclonal antibodies (mAbs) with the mutant lysozyme did not critically reduce, remaining 60% of the binding with mAb. In contrast, glycosylated mutant lysozyme G49N whose glycine was substituted with asparagine dramatically reduced the binding with mAb. The oligomannosyl type of G49N lysozyme reduced binding with mAb to one-fifth, while the polymannosyl type of G49N lysozyme completely diminished the binding with mAb. This suggests that the site-specific glycosylation of lysozyme in the interfacial region of lysozyme-antibody complex is more effective to reduce the antigenicity than the mutation of single amino acid substitution in the interfacial region.


Subject(s)
Antigens/immunology , Muramidase/chemistry , Muramidase/immunology , Amino Acid Substitution , Animals , Antibodies, Monoclonal , Antigen-Antibody Complex , Antigens/chemistry , Antigens/genetics , Chickens , Epitopes/analysis , Female , Glycosylation , Male , Mice , Mice, Inbred BALB C , Models, Molecular , Muramidase/genetics , Mutagenesis, Site-Directed , Protein Conformation
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