ABSTRACT
Natural complex compounds with hipolipidemic activity, having considerable inhibitory effect on expression of bacteria virulence factors were isolated. Inhibitory properties of the compounds with respect to pyocyanine and protease formation, as well as their influence on the quorum sensing mechanism in Chromobacterium violacium were shown.
Subject(s)
Actinobacteria , Chromobacterium , Pseudomonas aeruginosa , Pyocyanine , Quorum Sensing/drug effects , Actinobacteria/chemistry , Actinobacteria/metabolism , Chromobacterium/drug effects , Chromobacterium/metabolism , Complex Mixtures/pharmacology , Peptide Hydrolases/metabolism , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/enzymology , Pyocyanine/analysis , Pyocyanine/antagonists & inhibitors , Pyocyanine/metabolism , Quorum Sensing/physiology , Virulence Factors/antagonists & inhibitors , Virulence Factors/metabolismABSTRACT
Telomerase is a ribonucleoprotein that synthesizes telomeric repeats and identified as a promising target for anticancer therapy. Here we describe a new compound aITEL1296 as a potent telomerase inhibitor. Its inhibitory activity was a bit higher (IC50 = 0,19 +/- 0,02 ng/ml) than that of BIBR1532, one of the most potent telomerase inhibitors known to date. Besides telomerase inhibition aITEL1296 activated apoptotic mechanisms and effectively suppressed proliferation of tumor cell lines (GI50 = 5,0 +/- 0,2 ng/ml for most sensitive cell line LnCap) but not normal fibroblast cell line.
