ABSTRACT
Conveniently and efficiently monitoring I- and Hg2+ in agricultural products or drinking beverages for the protection of human health is currently a great challenge. With this aim, a Ag-Pt bimetal quantum-dot nanozyme boosted by bioactive folic acid (FA@Ag-Pt QDs) was first developed for multichannel monitoring of I- and Hg2+ in this work using a two-step liquid-phase reduction method. Not only did the present FA@Ag-Pt QDs possess superior peroxidase-like activity with Michaelis constant (Km) and maximal reaction rate (Vmax) of 0.01 mM/2.95 × 10-8 M·s-1 and 1.15 mM/3.88 × 10-8 M·s-1, respectively, trace Hg2+ or I- could exclusively alter their enzyme-mimic performance with obvious color changes from blue to colorless or dark blue. I- could also strengthen the inherent fluorescence property of FA@Ag-Pt QDs. When applied for visual monitoring of I- and Hg2+ in real beverages or iodine-containing agricultural products, the detection recoveries were 93.9 %-105.3 % and 96.8-104.3 % with low detection limits of 6.56 × 10-8 mol/L and 4.00 × 10-10 mol/L (S/N = 3), respectively. The recovery and detection limit for fluorescent detection of I- were 95.8 %-104.1 % and 1.75 × 10-8 mol/L (S/N = 3), respectively. The mechanisms driving the improved peroxidase-like activity of FA@Ag-Pt QDs and their selective monitoring of Hg2+ and I- were illustrated in detail. The proposed FA@Ag-Pt QDs will act as an efficient sensor for the practical multichannel monitoring of Hg2+ and I-, with superior catalytic signal amplification.
Subject(s)
Mercury , Quantum Dots , Humans , Colorimetry , Limit of Detection , Beverages , Coloring Agents , Mercury/analysis , PeroxidasesABSTRACT
Dopamine (DA) is an important neurotransmitter; however, any excess or deficiency of DA will cause several diseases in humans. To monitor DA efficiently and conveniently, a Ag nanozyme strengthened by bioactive folic acid (FA@AgNPs) was developed by homogeneous redox assembly. After the microstructure and performance were characterized in detail, it was noted that the proposed FA@AgNPs possessed superior peroxidase-like activity due to the ultra-small Ag nanoparticles and multiple amino, hydroxyl, and aromatic rings in FA. FA@AgNPs accelerated the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) with a low Michaelis constant (Km) and high maximal reaction rate (Vmax). Importantly, the characteristic absorbance intensity of FA@AgNPs-TMB-H2O2 at 652 nm (A652) was exclusively deteriorated in the presence of trace DA, accompanied by a visual color change from blue to colorless. Under the optimized conditions (pH 4.0, 300 µL 1.5 mM TMB, 300 µL 1.0 M H2O2 and incubated for 30 min at room temperature), there expressed an excellent linear relationship between lgA0/A652 and cDA from 1.0 ×10-8 to 6.67×10-6 mol/L with a low limit detection of 7.1×10-10 mol/L (S/N=3). When applied for monitoring of DA in real fruit juice and pharmaceutical samples, the recovery was between 96.6% and 104.9%, with RSD less than 2.2%. The enhanced peroxidase-like activity of the FA@AgNP system and its selective recognition mechanism for DA are also proposed.
