The discovery of deep-water seagrass meadows in a pristine Indian Ocean wilderness revealed by tracking green turtles.

Our understanding of global seagrass ecosystems comes largely from regions characterized by human impacts with limited data from habitats defined as notionally pristine. Seagrass assessments also largely focus on shallow-water coastal habitats with comparatively few studies on offshore deep-water seagrasses. We satellite tracked green turtles (Chelonia mydas), which are known to forage on seagrasses, to a remote, pristine deep-water environment in the Western Indian Ocean, the Great Chagos Bank, which lies in the heart of one of the world's largest marine protected areas (MPAs). Subsequently we used in-situ SCUBA and baited video surveys to survey the day-time sites occupied by turtles and discovered extensive monospecific seagrass meadows of Thalassodendron ciliatum. At three sites that extended over 128 km, mean seagrass cover was 74% (mean range 67-88% across the 3 sites at depths to 29 m. The mean species richness of fish in seagrass meadows was 11 species per site (mean range 8-14 across the 3 sites). High fish abundance (e.g. Siganus sutor: mean MaxN.site-1 = 38.0, SD = 53.7, n = 5) and large predatory shark (Carcharhinus amblyrhynchos) (mean MaxN.site-1 = 1.5, SD = 0.4, n = 5) were recorded at all sites. Such observations of seagrass meadows with large top predators, are limited in the literature. Given that the Great Chagos Bank extends over approximately 12,500 km2 and many other large deep submerged banks exist across the world's oceans, our results suggest that deep-water seagrass may be far more abundant than previously suspected.

The emergent role of exosomes in glioma.

Extracellular vesicles (EVs) are known mediators of intercellular communication for both normal and tumour cells. With the capability to transfer nucleic acids, proteins and lipids, EVs are able to influence numerous functional and pathological aspects of both donor and recipient cells. The tumour microenvironment possesses a high level of complex heterogeneity, particularly within the most prominent brain malignancy, glioblastoma multiforme (GBM). This complexity relies on a network-based communication between many different components of the local niche, including the various cell types, stroma, blood vessels, secreted factors and surrounding matrix. Exosomes are one type of EV which facilitates this intercellular communication and cross-talk within the tumour microenvironment. Exosomes secreted by tumour cells are increasingly recognized in a number of processes underlying tumour progression including facilitating the transport of receptors, signalling molecules, oncogenic genes and miRNA. They are emerging as a key component in the biogenesis of glioma, in addition to contributing to the modification of the surrounding microenvironment to support tumour progression. In this review we describe advancements in the understanding of the biology of exosomes, as well as their roles in tumour progression, as a tumour biomarker for tracking cancer progression, and as a potential therapeutic target/delivery system, with a contextual emphasis on GBM.

Low-order adaptive optics for free-space optoelectronic interconnects.

The concept of adaptive optics for improving the cost-performance of free-space optoelectronic interconnects is discussed. Adaptive optics as a design option for optical interconnect systems is presented and discussed. A practical demonstrator that performs low-order correction was built and tested. Slowly varying misalignments, including thermal effects, were compensated for in a 622-Mbit/s free-space optical data link.

Real-time optical aberration correction with a ferroelectric liquid-crystal spatial light modulator.

Real-time correction of an optically aberrated wave front by use of a 10 x 10 ferroelectric liquid-crystal spatial light modulator as the correction device and a point-diffraction interferometer as the wave-front sensor is demonstrated. This type of interferometer requires no reference arm and so can be used, in theory, in an astronomical adaptive-optics system. We discuss some of the unusual features of the point-diffraction interferometer for wave-front sensing.

Optoelectronic Systems Based on InGaAs- Complementary-Metal-Oxide-Semiconductor Smart-Pixel Arrays and Free-Space Optical Interconnects.

Free-space optical interconnects have been identified as a potentially important technology for future massively parallel-computing systems. The development of optoelectronic smart pixels based on InGaAs/AlGaAs multiple-quantum-well modulators and detectors flip-chip solder-bump bonded onto complementary-metal-oxide-semiconductor (CMOS) circuits and the design and construction of an experimental processor in which the devices are linked by free-space optical interconnects are described. For demonstrating the capabilities of the technology, a parallel data-sorting system has been identified as an effective demonstrator. By use of Batcher's bitonic sorting algorithm and exploitation of a perfect-shuffle optical interconnection, the system has the potential to perform a full sort on 1024, 16-bit words in less than 16 mus. We describe the design, testing, and characterization of the smart-pixel devices and free-space optical components. InGaAs-CMOS smart-pixel, chip-to-chip communication has been demonstrated at 50 Mbits/s. It is shown that the initial system specifications can be met by the component technologies.

Intensity-only modulation for atmospheric scintillation correction by liquid-crystal spatial light modulators.

We consider the use of a ferroelectric liquid-crystal spatial light modulator (FLC SLM) to control the transmittance of a telescope pupil to compensate for the effects of scintillation. Our aim here is to prove the necessary and physically interesting result that it is possible to control the intensity of light by use of FLC SLM without inducing further phase aberrations. Furthermore, we show that system errors have only a small effect on the phase of the transmitted beam.

Measurements on ferroelectric liquid-crystal spatial light modulators: contrast ratio and speed.

The contrast ratio and the speed of a 16 × 16 electrically addressed spatial light modulator, composed of a ferroelectric liquid-crystal layer on top of a VLSI silicon backplane, are measured with different methods but consistent results. The results are presented and compared with recently reported results on a similar spatial light modulator [Appl. Opt. 33, 2775 (1994)].

Improving the performance of liquid-crystal-over-silicon spatial light modulators: issues and achievements.

The performance of liquid-crystal-over-silicon spatial light modulators has advanced rapidly in recent years. Most progress has centered around new device designs with increased bandwidth. In this paper we report on a number of techniques to improve the optical quality; these have applications in both current and future devices.

Real-time binary phase holograms on a reflective ferroelectric liquid-crystal spatial light modulator.

A ferroelectric liquid-crystal spatial light modulator with an active silicon backplane is used to implement reconfigurable reflective phase holograms. Optical results are presented for an optimized computergenerated Fourier hologram.

Time-multiplexed optical Hadamard image transforms with ferroelectric-liquid-crystal-over-silicon spatial light modulators.

The Hadamard transform is an example of a matrix transform that allows images to be represented in terms of orthogonal basis functions with binary-valued matrix elements. Such basis functions can be displayed on binary amplitude modulating ferroelectric-liquid-crystal-over-silicon spatial light modulators, permitting novel, real-time, and high-speed implementation of the transform on incoherently illuminated input scenes. An example of an optical Hadamard transform performed in real time by using a spatial light modulator is described. To show the validity of this optical decomposition, we electronically reconstruct the transformation data for comparison with the original input scene.

Canine distemper virus titration in ferret peritoneal macrophages.

The sensitivity of a ferret peritoneal macrophage fluorescent antibody technique for assay of various strains of canine distemper virus was investigated. The macrophage system was compared with established methods of titration in canine kidney cell culture, Vero cell culture, and embryonated chicken eggs. It was found to be as sensitive as and in several instances more sensitive than the established methods.

Simultaneous use of contrasting fluorochromes to separate measles and canine distemper viruses in a common system.

Measles and canine distemper viruses were grown together in a Vero monkey kidney cell line. Each virus could be identified and individually titrated by using the color contrast produced by the reddish tetra-methyl rhodamine isothiocyanate-tagged antimeasles conjugate and the green fluorescein isothiocyanate-tagged antidistemper conjugate. Both blue light and green light were used for the excitation of the fluorochromes. Incident light was transmitted to the specimen by a vertical illuminator of the Ploem type.