The Effect of Formulation, Dose, and Adjuvants on Uptake of Phosphite Into Pine Foliage.

The aim of this investigation was to determine the effect of dose and adjuvant on uptake of two phosphite products (Phos-A and Phos-B) into Pinus radiata needles. In experiment 1, uptake of 6 kg ha-1 phosphite, applied as Phos-A, in 100 liters of water, together with an organosilicone superspreader (0.2%), was high (>60%). Uptake at doses greater than 6 kg ha-1 (12, 15, 18, and 24 kg ha-1) and applied in volumes less than 100 liters of water (75 and 50 liters) was poor (1 to 30%). Using stability tests and NMR spectroscopy in experiment 2, this appeared to be linked to a concentration dependent reaction resulting in the degradation of the organosilicone adjuvant that facilitated uptake of Phos-A. In experiment 3, uptake of phosphite applied as Phos-B, between 6 and 24 kg ha-1 in 100 liters of water, was tested alone and with four adjuvants (an organosilicone, alcohol ethoxylate, lecithin, and esterified seed oil). Uptake of Phos-B without any adjuvant was high (>50%) across all doses, indicating the formulation was optimized for P. radiata needles. Uptake of Phos-B increased with concentration up to 72% at 24 kg ha-1 in 100 liters of water. Symptoms of phytotoxicity were observed at rates of ≥12 kg ha-1. This study highlighted the effect of formulation, dose, concentration, and adjuvant on the uptake of phosphite into P. radiata needles.

An aerial spot-spraying technique: a pilot study to test a method for pest eradication in urban environments.

BACKGROUND: Pest eradication strategies that use pesticides require application methods that have the lowest environmental and human health impact while maintaining the highest probability of success. This is highly important when eradication takes place in sensitive areas, such as urban or riparian zones. A new aerial application method, the spot-gun, was developed to provide good pesticide coverage on host species while minimising off-target exposure. This type of targeted aerial approach is useful in areas where conventional broadcast aerial spraying was historically used but was not ideal due to the quantity of pesticide hitting non-host species and going off-target. An even distribution of the active component is essential for eradication. FINDINGS: The spot-gun method was tested and found to provide an even distribution of dye on the adaxial and abaxial leaf surfaces as well as in the top and middle regions in both the inner and outer portions of the canopy. The form of the deposits on the leaf surface was very different from that obtained using a broadcast aerial application with a conventional spray boom. CONCLUSIONS: The distribution results imply that the spot-gun method treatment will provide good efficacy. The implications of the different deposit pattern on efficacy are not known at this stage. This aerial spot-spray method has considerable potential as a tool for targeted aerial application of pesticides to small areas of difficult to reach canopies.

Aerial application of pheromones for mating disruption of an invasive moth as a potential eradication tool.

Biological invasions can cause major ecological and economic impacts. During the early stages of invasions, eradication is desirable but tactics are lacking that are both effective and have minimal non-target effects. Mating disruption, which may meet these criteria, was initially chosen to respond to the incursion of light brown apple moth, Epiphyas postvittana (LBAM; Lepidoptera: Tortricidae), in California. The large size and limited accessibility of the infested area favored aerial application. Moth sex pheromone formulations for potential use in California or elsewhere were tested in a pine forest in New Zealand where LBAM is abundant. Formulations were applied by helicopter at a target rate of 40 g pheromone per ha. Trap catch before and after application was used to assess the efficacy and longevity of formulations, in comparison with plots treated with ground-applied pheromone dispensers and untreated control plots. Traps placed at different heights showed LBAM was abundant in the upper canopy of tall trees, which complicates control attempts. A wax formulation and polyethylene dispensers were most effective and provided trap shut-down near ground level for 10 weeks. Only the wax formulation was effective in the upper canopy. As the pheromone blend contained a behavioral antagonist for LBAM, 'false trail following' could be ruled out as a mechanism explaining trap shutdown. Therefore, 'sensory impairment' and 'masking of females' are the main modes of operation. Mating disruption enhances Allee effects which contribute to negative growth of small populations and, therefore, it is highly suitable for area-wide control and eradication of biological invaders.

Stem injection of a systemic insecticide to control Uraba lugens on urban Lophostemon confertus trees.

BACKGROUND: Uraba lugens Walker (Lepidoptera: Nolidae) has the potential to become a serious pest of susceptible forestry and urban trees in New Zealand. Stem injection of insecticides provides a method for the control of U. lugens in public locations where foliar applications are not suitable. Field and laboratory studies were conducted in 2009 and 2010 to determine a dose response profile of U. lugens larvae to acephate injected at 0.0, 0.25, 0.50 and 1.00 g cm(-1) tree diameter measured at breast height (dbh). The concentration of the injected solution varied across years. RESULTS: The highest mortality (85-100%) of U. lugens larvae occurred at 1.00 g acephate injected cm(-1) dbh. The lower rates (0.25 and 0.50 g acephate cm(-1) ) were more effective in 2010 than in 2009, a factor attributed to the change in volume of injected solution. CONCLUSION: Systemic injections of acephate at rates above 0.50 g cm(-1) dbh provide a tool for the management of U. lugens in public places. It is recommended that stem injection operations for control of U. lugens larvae be timed to coincide with the emergence of first instars so that larvae are exposed to lethal insecticide doses.