ABSTRACT
Phthalimido-alkyl alcohol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 13-aminoalkyl oligodeoxynucleotides allowing a two step deprotection necessary to avoid aminolink alkylation.
Subject(s)
Microwaves , Molecular Biology/methods , Oligonucleotides/chemical synthesis , Chromatography, High Pressure Liquid , DNA/chemistry , Models, Chemical , Oligonucleotides/chemistry , Oligonucleotides/radiation effects , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Time Factors , Ultraviolet RaysABSTRACT
Phthalimido-alkanol solid supports were rapidly prepared from solid supported phthalic anhydride and amino alcohol condensation induced by microwaves. These supports were used to synthesize 5'-fluorescent 3'-aminoalkyl oligodeoxynucleotides allowing a two-step deprotection necessary to avoid aminolink alkylation. After conversion into an NHS derivative using dissuccinimidyl suberate and an optimized isolation, they were conjugated with a protein.
