ABSTRACT
The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 µM 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis.
Foram investigados os efeitos de diferentes tratamentos de qualidade da luz sobre o crescimento e multiplicação in vitro de cana-de-açúcar (RB867515). As plantas foram cultivadas em meio MS contendo 1,3 µ M de 6-benzilaminopurina (BAP) e expostas a cinco tratamentos de luz: três combinações de LED azul/vermelho (70:30, 50:50, 30:70), LED branco e lâmpadas fluorescentes, durante 24 dias. Entre os tratamentos de luz de LED, a combinação azul/vermelho na proporção 50:50 apresentou os melhores resultados para altura, massa fresca, número de folhas e multiplicação de brotos. Maior teor de pigmentos fotossintéticos também foi obtido com a utilização de LEDs. Os resultados sugerem que a qualidade da luz emitida pelos LEDs foi adequada para o crescimento e desenvolvimento das plantas e pode ser utilizada como fonte de luz alternativa e econômica para a micropropagação da variedade de cana-de-açúcar estudada.
Subject(s)
Saccharum/growth & development , In Vitro Techniques/methods , LightABSTRACT
The recent development of core/shell engineering of rare earth doped luminescent nanoparticles has ushered a new era in fluorescence thermal biosensing, allowing for the performance of minimally invasive experiments, not only in living cells but also in more challenging small animal models. Here, the potential use of active-core/active-shell Nd(3+)- and Yb(3+)-doped nanoparticles as subcutaneous thermal probes has been evaluated. These temperature nanoprobes operate in the infrared transparency window of biological tissues, enabling deep temperature sensing into animal bodies thanks to the temperature dependence of their emission spectra that leads to a ratiometric temperature readout. The ability of active-core/active-shell Nd(3+)- and Yb(3+)-doped nanoparticles for unveiling fundamental tissue properties in in vivo conditions was demonstrated by subcutaneous thermal relaxation monitoring through the injected core/shell nanoparticles. The reported results evidence the potential of infrared luminescence nanothermometry as a diagnosis tool at the small animal level.
