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Article in Russian | MEDLINE | ID: mdl-66827

ABSTRACT

A possibility was shown of using the phospholipid group-specific antigen of the ornitosis causative agent in the capacity of a hemosensitin for conducting the indirect hemagglutination test (IHAT). There was revealed a direct proportional relationship between the sensitizing activity and the complement fixing properties of the antigens obtained. A method of sorption of this antigen on the tannin-treated erythrocytes and the optimal parameters of conducting the test with it were elaborated. A sufficiently high sensitivity and specificity of the IHAT was revealed as a result of parallel titration of hetero-and homologous sera in the complement fixation test and in the IHAT. The suggested test can be successfully used for the diagnosis of ornithosis.


Subject(s)
Antigens, Bacterial , Chlamydophila psittaci/immunology , Phospholipids/immunology , Psittacosis/diagnosis , Complement Fixation Tests/methods , Epitopes , Hemagglutination Tests/methods , Humans
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