ABSTRACT
Puerarin accumulation during shoot cultures in static and liquid medium with or without aeration is described in Pueraria tuberosa (Roxb. ex Willd.) DC. Maximum shoot induction from nodal explants was achieved on Murashige and Skoog's medium supplemented with 1.13 µM TDZ and 0.25 µM IBA after 4 weeks of growth. Puerarin content was higher in shoot cultures grown in liquid medium as compared to static medium. When shoots were grown in growtek bioreactor with different aeration volume, maximum puerarin content (1484 µg/g DW) was recorded with 20 % v/v aeration which was ~2.3 fold higher than puerarin content recorded in control cultures (cultures grown in growtek without aeration). Aeration requirement for organized (shoot) cultures was different for growth and puerarin accumulation.
ABSTRACT
Suspension cultures of Pueraria tuberosa, a woody legume, have been established and using different concentrations of growth regulators, sucrose, ammonium and nitrate nitrogen, attempts have been made to improve their isoflavonoid content. The cell cultures grew well on all the treatments. Up to approximately 8 folds increased isoflavonoids content was recorded in the cultures grown in MS medium modified with nitrogen and supplemented with 1 mg 1(-1) of kinetin.
Subject(s)
Culture Media/chemistry , Isoflavones/metabolism , Pueraria/metabolism , Biomass , Cell Culture Techniques , Cells, Cultured , Culture Media/metabolism , Pueraria/cytologyABSTRACT
Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of P. tuberosa was studied by manipulating the plant growth regulators and sucrose concentration in the medium. Organogenesis was not recorded in callus on any of these treatments. Tuber and stem accumulated puerarin, a glycoside of daidzein, at high amounts, 0.65% and 0.054% respectively. However, the daidzein content of the callus tissues grown on Murashige and Skoog medium containing BA (20.9 microM) and sucrose (60 gl(-1)) was significantly higher (0.056%) than in vivo plant material (0.02%) and other comparable culture systems like Genista and Pueraria lobata.
