ABSTRACT
Peptidatic activity of meconium of 200 newborns and 100 preterm babies. Protein content of meconium is higher in preterm babies than in healthy newborns referred to fresh weight. Enzymatic activity opposite to Gly-D-Leu is higher in term than in preterm newborns. It is on the contrary in the case of alanine-aminopeptidase, dipeptidyl-peptidase IV and the activities opposite to Leu-NA and Arg-NA. The good correlated enzymatic activities opposite the different substances give a hint to the presence of alanine-amino-peptidase (EC 3.4.11.2.)
Subject(s)
Infant, Premature/physiology , Meconium/enzymology , Peptide Hydrolases/metabolism , Child, Preschool , Female , Follow-Up Studies , Humans , Infant , Infant, Newborn , Male , Reference ValuesABSTRACT
Aminoacylase from Euglena gracilis was purified by gel filtration and DEAE-Sephacel 230 times and analyzed with respect to substrate specificity, effector behaviour, pH optimum, Michaelis constant, electrophoretic mobility and molecular weight. The molecular weight is about 252000 (gel filtration). The properties of the aminoacylase from Euglena gracilis and the acylases I (EC 3.5.1.14) from the human and the pig kidneys were compared. The enzyme could be distinguished immunologically from the renal aminoacylases. No indications for the existence of a separate acylase III or of a deformylase were found.
Subject(s)
Amidohydrolases/metabolism , Euglena gracilis/enzymology , Amidohydrolases/isolation & purification , Electrophoresis, Polyacrylamide Gel , Immune Sera , Immunodiffusion , Kinetics , Molecular Weight , Substrate SpecificityABSTRACT
8 patients with chronic pyelonephritis were given gentamycin intramuscularly injected in individual dosage during 8-10 days. Here the behaviour of the excretion of protein, alanine aminopeptidase alkaline phosphatase, alpha-glucosidase, gamma-glutamyl transpeptidase and lysozyme with the urine was tested. With the exception of the lysozymuria, which increased only in patients with chronic renal insufficiency, regularly a hyperenzymuria developed. Most distinctly the excretion of the alanine aminopeptidase increased. After initial decrease the excretion of total protein transiently increased after completion of the gentamycin therapy. All the deviations were reversible. From the increased excretion of enzymes may not be concluded to a nephrotoxicity of gentamycin.
