ABSTRACT
Aim: To study the effect of antibiotic exometabolites of Bacillus amyloliquefaciens subsp. plantarum IMV B-7524 strain on the induction of defense responses in young plants of winter wheat. Methods: Microbiological, biochemical, phytopathological, statistical. Results: It was found that, when pre-watering winter wheat plants with salicylic acid, the cell-free culture supernatant and lipopeptide fraction of IMV B-7524 strain exometabolites during the further infection with phytopathogen Bipolaris sorokiniana, the damaged surface area of leaves decreased more than 10 times compared to the control. It was shown that in the roots and shoots of winter wheat plants L-phenylalanine ammonia-lyase (PAL) activity increased about 3 times in 2 hours after treatment. Conclusion: Reduction of the damaged surface area of leaves by the action of fungal phytopathogen and change of PAL activity in plants indicate the induction of defense responses under the influence of antibiotic exometabolites of B. amyloliquefaciens subsp. plantarum IMV B-7524 strain.
Subject(s)
Bacillus amyloliquefaciens/chemistry , Plant Diseases/microbiology , Triticum/physiology , Fungi/pathogenicity , Plant Leaves/microbiology , Plant Leaves/physiology , Triticum/microbiologyABSTRACT
The polyphasic taxonomic analysis of the strain-antagonist of phytopathogenic bacteria and micromycetes was carried. It was found that the combination of culture-morphological, physiological and biochemical properties allowed to attribute the strain to the group Bacillus subtilis. It was shown that fatty acids of the cell walls of the strain were represented mainly by branched derivatives of iso- and anti iso- C15:0 and C17:0 fatty acids (85%) which was typical for the Bacillus amyloliquefaciens species. After molecular genetic analysis of the nucleotide sequence of the 16S rRNA gene and studying the profile of polymorphic nucleotides the strain was attributed to subspecies Bacillus amyloliquefaciens subsp. plantarum.
Subject(s)
Bacillus amyloliquefaciens/genetics , Bacillus subtilis/genetics , DNA Barcoding, Taxonomic/methods , DNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Antibiosis/genetics , Bacillus amyloliquefaciens/classification , Bacillus amyloliquefaciens/metabolism , Bacillus subtilis/classification , Bacillus subtilis/metabolism , Bacterial Typing Techniques , Cell Wall/chemistry , Fatty Acids/classification , Fatty Acids/isolation & purification , Fatty Acids/metabolism , PhylogenyABSTRACT
Antagonistic activity 100 strains of Bacillus bacteria towards to museum and actual strains of phytopathogenic bacteria and fungy was defined. Relation between level of antagonistic activity to phytopathogenic bacteria and genus accessory of the last was shown. The medium level of antagonism to fungal phytopathogens at 30% of the studied strains of Bacillus bacteria was shown. 5 strains of Bacillus sp. with high and medium levels of antagonism to phytopathogens bacterial and fungy nature was selected and considered as perspective for creation of biological preparations for plant protection.
