ABSTRACT
BACKGROUND: The hepatitis C virus (HCV) genomic database is expanding rapidly. AIMS: There is a need to provide an updated phylogenetic tree analysis based on the complete coding region of HCV. METHODS: All available HCV complete genome sequences in the HCV databases available through October 2010 were analyzed. RESULTS: The assignment of all known complete sequences up-to-date confirmed the previous six major genotypes and one new sequence, which have been provisionally assigned as subtype 7a. New recombinant forms of HCV, although uncommon, have been detected and were found to have different crossover points. CONCLUSION: This updated analysis based on the complete region of HCV confirmed the validity of the previously assigned genotypes/subtypes and provided an up-to-date reference for future basic research and clinical studies.
Subject(s)
Genes, Viral , Hepacivirus/genetics , RNA, Viral/genetics , Databases, Factual , Genome, Viral , Genotype , Hepacivirus/classification , PhylogenyABSTRACT
The intervention program described began with teaching patients in simple terms about PVD, associated risk factors, and why change is important. Teaching aids such as posters and booklets were used to supplement discussion. Positive reinforcement was emphasized. Phone calls, follow-up visits, encouragement and praise were frequent. Newsletters focusing on different aspects of the various risk factors were developed and mailed monthly. Patients were encouraged and helped to develop an individualized program of change based on discussions and practicality. They were encouraged to start slowly and increase gradually. Emphasis was placed on reinforcement, self-help, and praise for efforts. Patients were encouraged to keep a brief daily log of progress and thoughts. Regularly scheduled physical assessments provided a tool to assess change and provide tangible evidence of their ability to set a goal, follow through, and make life-style change.
