ABSTRACT
A substrate specificity of RNAses of A. laidlawii PG-8 to polynucleotides - poly (C), poly (U), poly (A) has been studied. Due to the data obtained both intracellular and extracellular RNAses of A. laidlawii possess similar specificity to different polynucleotides. Both RNAses preferentially break cytidine-bonds. Specificity of the studied enzymes in respect to polyuridylic and polyadenylic acids was less expressed.
Subject(s)
Acholeplasma laidlawii/enzymology , Endoribonucleases/metabolism , Exoribonucleases/metabolism , Poly A/metabolism , Poly C/metabolism , Poly U/metabolism , Substrate SpecificitySubject(s)
Plant Viruses/genetics , Base Sequence , Genes, Viral/genetics , Molecular Sequence Data , Plant Viruses/classification , Plant Viruses/pathogenicity , Plant Viruses/ultrastructure , Protein Biosynthesis , RNA, Viral/genetics , Viral Proteins/genetics , Virion/ultrastructure , Virus ReplicationABSTRACT
Amino acid sequences of coat protein of potato X-virus, aucuba potato mosaic, papaya mosaic and white clover mosaic have been comparatively studied. The results of this comparative study have become the basis for prediction of the secondary structure of the viruses and for construction of a model of potexvirus coat protein. The potexvirus coat protein was based on the three-layered arrangement of five alpha spirals. It is supposed that RNA molecule is within the protein subunit. Availability of common amino acid sequences in alpha spirals of coat proteins of TMV and potexviruses is established and this permits supposing their common origin.
