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1.
Vet J ; 240: 47-49, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30268333

ABSTRACT

In the period from October 2016 to February 2017, the urinary tracts of 390 wild boar (Sus scrofa) from four areas of south central Spain (102 from Doñana National Park; 150 from Sierra Morena and the Toledo Mountains; 84 from Sierra Nevada; 54 from Sierra de Cazorla, Segura y Las Villas Natural Park) were examined for the presence of adult specimens of Stephanurus dentatus (Nematoda: Strongyloidea). This parasite was only detected in the wild boar population of Doñana National Park, with high prevalence (76.5±4.2%; 78/102), mean intensity (43.2±4.4) and mean abundance (33.1±3.8). Juvenile wild boar had significantly lower prevalence and abundance than subadult and adult wild boar. The intensity of infestation was significantly higher in male than in female wild boar. The detection of a focus of S. dentatus infestation in the wild boar population in Doñana National Park will provide further opportunities for understanding the epidemiology of this parasite.


Subject(s)
Strongylida Infections/veterinary , Strongyloidea/isolation & purification , Sus scrofa/parasitology , Swine Diseases/epidemiology , Animals , Female , Male , Prevalence , Spain/epidemiology , Strongylida Infections/epidemiology , Swine
2.
Vet Parasitol ; 164(2-4): 340-3, 2009 Oct 14.
Article in English | MEDLINE | ID: mdl-19596519

ABSTRACT

The present investigation examined the prevalence and abundance of bronchopulmonary nematodes in 213 randomly hunted Iberian ibexes (Capra pyrenaica) (87 females and 126 males) in the Sierra Nevada mountain range in Spain between 2003 and 2006. Post mortem examination revealed an overall prevalence of 72% for adult nematodes (Cystocaulus ocreatus 44%, Muellerius capillaris 44%, Protostrongylus sp. 40%, and Dictyocaulus filaria 4%). The abundances were 13.45+/-3.97, 5.18+/-2.49, 6.36+/-2.16, and 2.27+/-0.46, respectively. Protostrongylid adults showed similar infection rates, which were statistically different from that of D. filaria. 20% of the examined Iberian ibexes were infected by three protostrongylid nematodes species, 24% of C. pyrenaica were affected by two protostrongylid species, while infestations with only one protostrongylid species were detected in 20% of the examined animals. The overall prevalence of larvae nematodes in the examined animals was 100%, and the overall abundance (number of the first stage larvae per gram) was 86.45+/-20.63. There was a high correlation between the two sets of data (adults and larvae). Results of the present investigation provided foundation for the effective control of bronchopulmonary nematode infection in Iberian ibex.


Subject(s)
Goat Diseases/parasitology , Lung Diseases, Parasitic/veterinary , Nematode Infections/veterinary , Animals , Female , Goat Diseases/epidemiology , Goats , Lung Diseases, Parasitic/epidemiology , Lung Diseases, Parasitic/parasitology , Male , Nematoda/classification , Nematoda/isolation & purification , Nematode Infections/epidemiology , Nematode Infections/parasitology , Spain/epidemiology
3.
Parasitol Res ; 104(1): 101-5, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18758821

ABSTRACT

The objective of the present study was to examine the extent of genetic diversity among Sarcoptes scabiei individuals belonging to different skin subunits of the body from individual mangy hosts. Ten microsatellite primers were applied on 44 individual S. scabiei mites from three mangy Iberian ibexes from Sierra Nevada Mountain in Spain. Dendrograms of the mites from the individual Iberian ibexes, showing the proportion of shared alleles between pairs of individual mites representing three skin subpopulations (head, back, and abdomen subunits), allowed the clustering of some mite samples up to their skin subunits. This genetic diversity of S. scabiei at skin-scale did not have the same pattern in all considered hosts: for the first Iberian ibex (Cp1), only mites from the head subunit were grouped together; in the second individual (Cp2), the clustering was detected only for mites from the abdomen subunit; and for the third one (Cp3), only mites from the back subunit were clustered together. Our results suggest that the local colonization dynamics of S. scabiei would have influenced the nonrandom distribution of this ectoparasite, after a single infestation. Another presumable explanation to this skin-scale genetic structure could be the repeated infestations. To our knowledge, this is the first documentation of genetic structuring among S. scabiei at individual host skin-scale. Further studies are warranted to highlight determining factors of such trend, but the pattern underlined in the present study should be taken into account in diagnosis and monitoring protocols for studying the population genetic structure and life cycle of this neglected but important ectoparasite.


Subject(s)
Genetic Variation , Goat Diseases/parasitology , Goats/parasitology , Mite Infestations/veterinary , Sarcoptes scabiei/genetics , Skin/parasitology , Animals , DNA/analysis , Host-Parasite Interactions , Male , Microsatellite Repeats , Mite Infestations/parasitology , Polymerase Chain Reaction/methods , Sarcoptes scabiei/classification , Spain
4.
Parasitol Res ; 103(1): 181-6, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18368421

ABSTRACT

A collection of483 samples representing Fasciola from six naturally infected host species and 16 localities in Spain, previously identified morphologically and genetically as Fasciola hepatica, was characterized by a novel genetic marker, namely sequence-related amplified polymorphism (SRAP), aiming to reveal genetic variability within F. hepatica in Spain. Visualization of amplification fragments was carried out on 6% denaturing polyacrylamide gels, followed by staining with 0.1% AgNO3 solution. Ten SRAP primer combinations were tested--six of them turned out to be polymorphic. Thirty-four representative F. hepatica samples from six host species and 16 geographical localities showed polymorphic banding patterns using SRAP primer combinations and were grouped into four major clusters using the unweighted pair-group method with arithmetic averages, indicating the existence of genetic variability within the examined F. hepatica samples. These four clusters were not related to particular host species and/or geographical origins of the samples. The results of the present study revealed that SRAP markers were useful in revealing sufficient polymorphism in F. hepatica samples from Spain and had implications for studying the population genetic structure of the Spanish F. hepatica. To our knowledge, this is the first application of SRAP marker to study genetic variation in parasites of human and animal health significance.


Subject(s)
Fasciola hepatica/genetics , Genetic Markers , Genetic Variation , Helminth Proteins/genetics , Animals , Cattle/parasitology , Deer/parasitology , Demography , Gene Expression Regulation , Horses/parasitology , Phylogeny , Sheep/parasitology , Spain
5.
Parasitol Res ; 102(4): 751-5, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18092179

ABSTRACT

Between 1995 and 2006, we surveyed the presence of Fasciola hepatica in Iberian ibex (Capra pyrenaica) from Andalucía (southern Spain) by both necropsy (n = 2,096) and coprological approaches (n = 380). Most of the samples came from the Sierra Nevada mountain range (n = 1,884 and 267, respectively), and all positive cases involved animals from this location. The prevalence reached 0.53% by necropsy and 1.87% by faecal examination. Taking into account both diagnostic methodologies and the total number of animals affected (n = 14), we obtained a yearly prevalence of 0.7 +/- 0.3%. The infection with F. hepatica was found not to be related to host sex, climatology or to co-infection with Sarcoptes scabiei (the most important parasite affecting Iberian ibex, with a prevalence of 49.27 +/- 7.90% in the examined animals). The prevalence of fasciolosis decreased significantly during the period under study and this would be explained by an increase of ibex resistance to this fluke as a result of a reduction of the parasite abundance in the area and/or a reduction of the host infection rate. There was no statistical difference between the two diagnostic methods for the examination of fasciolosis during the period in which both methods were used. Therefore, examination of faecal samples as a non-invasive procedure may provide a useful approach for monitoring fasciolosis in wild ungulate populations. The results of the present study provided foundation for the effective control of F. hepatica infection in Iberian ibex.


Subject(s)
Fasciola hepatica/isolation & purification , Fascioliasis/veterinary , Goat Diseases/epidemiology , Goats/parasitology , Animals , Fascioliasis/epidemiology , Fascioliasis/parasitology , Female , Goat Diseases/parasitology , Male , Prevalence , Spain/epidemiology
6.
Parasitol Res ; 101(5): 1245-50, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17610081

ABSTRACT

In the present study, 25 samples representing Fasciola (Platyhelminthes: Trematoda: Digenea) from nine host species and 19 geographical locations in Spain were characterized genetically by sequences of the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA). The ITS rDNA was amplified from individual liver flukes by polymerase chain reaction (PCR), and the amplicons were sequenced directly. The lengths of the ITS-1 and ITS-2 sequences were 422 and 362 bp, respectively, for all Spanish liver fluke samples sequenced. Comparison of the ITS sequences of the Spanish Fasciola samples examined in the present study with that of Fasciola hepatica, Fasciola gigantica and the "intermediate Fasciola" revealed that all Spanish Fasciola samples examined represent the single species of F. hepatica, with only slight sequence variation in the ITS-2 (1/362, 0.3%) among the sequenced samples, but the sequence variation was not related to particular host species and/or geographical origins of the samples. The Spanish F. hepatica examined differed from Fasciola from elsewhere by two nucleotides in the ITS-2, which provided genetic marker for the differentiation of Spanish F. hepatica from Fasciola from other geographical localities. These results have implications for studying the population genetic structure of the Spanish F. hepatica and for the diagnosis and control of the disease it causes.


Subject(s)
Fasciola hepatica/classification , Fasciola hepatica/isolation & purification , Fascioliasis/parasitology , Animals , Animals, Domestic/parasitology , Animals, Wild/parasitology , DNA, Helminth/chemistry , DNA, Helminth/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Fasciola hepatica/genetics , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, Genetic , Sequence Analysis, DNA , Sequence Homology , Spain
7.
Parasite ; 13(4): 305-10, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17285851

ABSTRACT

Third-instar Oestrus caucasicus larvae (n = 236) obtained from Iberian ibex, Copra pyrenaica, were reared in a laboratory to obtain adult flies. They were maintained at a temperature of 21.9 +/- 2.7 degrees C and a relative humidity of 38.9 +/- 8.0 %. In all, 78 imagos emerged (33.1 %), with a sex-ratio at emergence not differing significantly from 1:1; 25 larvae did not complete pupariation. A total of 14 adult flies (17.9 % of the adults obtained) showed malformations, mainly in their wings. The pupariation period lasted around 30 hours and the pupal stage lasted on average 29.8 +/- 6.8 days. The success of pupation in both sexes was mainly determined by the weight of the larvae. Sexual dimorphism, with higher weights in females, was evident in third-instar arvae, pupae and adults. The mean longevity of adult flies was 224.8 +/- 91.4 hours and males generally survived for onger than the females.


Subject(s)
Diptera/growth & development , Goat Diseases/parasitology , Myiasis/veterinary , Animals , Body Weight/physiology , Diptera/anatomy & histology , Diptera/physiology , Female , Goats , Humidity , In Vitro Techniques , Larva/growth & development , Larva/physiology , Longevity , Male , Myiasis/parasitology , Sex Characteristics , Sex Ratio , Temperature , Time Factors
8.
J Wildl Dis ; 35(1): 78-81, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10073350

ABSTRACT

From February 1992 to March 1997, 245 European mouflon (Ovis orientalis musimon) from Sierras de Cazorla, Segura y Las Villas Natural Park (southern Spain) were surveyed for oestrid larvae in order to estimate prevalence and mean intensity of parasitism by Oestrus ovis. Over 46 percent of the animals surveyed were infected, with a mean intensity of 9.6 larvae/host parasitized. No significant differences in prevalence rates between host sexes were observed, but older mouflons were infected with more larvae than younger ones.


Subject(s)
Myiasis/veterinary , Sheep Diseases/epidemiology , Age Distribution , Animals , Female , Male , Myiasis/epidemiology , Prevalence , Sex Distribution , Sheep , Spain/epidemiology
9.
J Zoo Wildl Med ; 30(4): 550-4, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10749444

ABSTRACT

Thirteen hematologic parameters were measured in 52 Spanish ibexes (Capra pyrenaica) from Sierra Nevada Natural Park, southern Spain. The animals were captured, maintained in captivity, and physically restrained for blood collection. We compared hematologic values for healthy ibexes with animals infected with sarcoptic mites and animals treated for scabies and recovered. Ibexes with scabies showed a decreased number of erythrocytes, a higher mean corpuscular volume and mean corpuscular hemoglobin, and increased numbers of band neutrophils. The number of lymphocytes was significantly higher in animals that had recovered from scabies as compared with infested ones. The only difference observed between healthy and recovered animals was in the number of monocytes, which remained significantly higher in animals that had recovered from scabies. No hemoparasites were found in this study.


Subject(s)
Goat Diseases/blood , Goats/blood , Mite Infestations/veterinary , Animals , Animals, Wild , Female , Hematologic Tests/veterinary , Male , Mite Infestations/blood , Reference Values , Spain
10.
J Parasitol ; 82(2): 233-6, 1996 Apr.
Article in English | MEDLINE | ID: mdl-8604089

ABSTRACT

Prevalence and intensity of parasitism by oestrid larvae in the Spanish ibex Capra pyrenaica were determined for a period of 2.5 yr in Sierra Nevada Natural Park, southern Spain. The most relevant result of this study was to find Oestrus caucasicus parasitizing this ungulate in a geographical location far from its previously known distribution, e.g., the Caucasus mountain range and central Asia. Larvae were found within 74% of the heads examined (n = 180). Prevalence was higher in females and in older animals. Lowest prevalence occurred during the summer months. The mean intensity (+/- SD) was 25.4 +/- 27.3 larvae and was similar in both host sexes. The number of larvae increased with host age and reached the highest intensities in winter, during December and January.


Subject(s)
Diptera/physiology , Goat Diseases/epidemiology , Myiasis/veterinary , Age Distribution , Animals , Female , Frontal Sinus/parasitology , Goats , Larva/physiology , Male , Myiasis/epidemiology , Nasopharynx/parasitology , Paranasal Sinuses/parasitology , Prevalence , Seasons , Sex Distribution , Spain/epidemiology
11.
J Wildl Dis ; 31(4): 486-90, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8592379

ABSTRACT

From November 1992 to February 1993, 455 red deer (Cervus elaphus) were surveyed in order to estimate the prevalence of warble fly (Hypoderma actaeon) larvae under the skin of this ungulate species. Material came from Montes de Toledo, Sierra Morena, Sierra de Alcaraz, and Sierras de Cazorla, Segura y Las Villas Natural Park (central and southern Spain). We observed a prevalence of 92%, with a mean +/- SD intensity of 35.7 +/- 41.3 grubs per parasitized host; there was a maximum of 317 larvae per host. No significant differences in prevalence by host sex were found, although significant differences were observed in prevalences among different host age classes. The main location of feeding larvae in deer was in the back tissues.


Subject(s)
Deer/parasitology , Diptera/growth & development , Hypodermyiasis/veterinary , Age Distribution , Animals , Female , Hypodermyiasis/epidemiology , Larva/growth & development , Male , Prevalence , Seasons , Sex Distribution , Skin/parasitology , Spain/epidemiology
12.
Parassitologia ; 37(1): 45-51, 1995 Apr.
Article in English | MEDLINE | ID: mdl-8532366

ABSTRACT

Adult specimens of Laemobothrion (L.) maximum (Scopoli, 1763), a buzzard louse species, were studied using scanning electron microscopy and paying special attention to sensitive structures, mainly those located on the head, mouthparts, abdomen and legs. Data on shape and size of palpl and antennal sensilla, as well as post-spiracular ones, were obtained. Some modifications of the mouthparts were observed.


Subject(s)
Bird Diseases/parasitology , Birds/parasitology , Lice Infestations/veterinary , Phthiraptera/anatomy & histology , Animals , Feathers/parasitology , Lice Infestations/parasitology , Microscopy, Electron, Scanning , Phthiraptera/ultrastructure
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