Inverting nutrient fluxes across the land-water interface - Exploring the potential of zebra mussel (Dreissena polymorpha) farming.

We studied the potential of zebra mussel farming for nutrient retention in a eutrophic lake. Duplicate experimental long-line cultivation units were deployed and mussel growth and nutrient retention were quantified after 28 months. Mussels grew well at shallow water depth (<3 m) and our 625 m2 (lake area) experimental units produced 507 and 730 kg dry biomass, respectively, of which 94% were shells. These yields corresponded to an average retention of 92.7 ± 23.1 kg C, 6.1 ± 0.68 kg N, and 0.43 ± 0.04 kg P retention, or 742 kg C, 49 kg N, and 3.5 kg P for a full-size (0.5 ha) mussel farm. We estimate that concentrating the long-lines to a depth of 2.5 m would probably have doubled these yields, based on the differences in mussel growth among depths. We further estimate that a full-size cultivation unit (0.5 ha) thus could compensate for the annual total-P run-off from 23 ha, or the biologically available P from approximately 49 ha of agricultural soils. As traditional measures have proven insufficient, decision-makers need to facilitate novel approaches to mitigate the negative effects of cultural eutrophication. We envision that zebra mussel farming, within their invaded range, provides a promising approach to invert nutrient losses in lakes and coastal lagoons.

Assessing critical load exceedances and ecosystem impacts of anthropogenic nitrogen and sulphur deposition at unmanaged forested catchments in Europe.

Anthropogenic emissions of nitrogen (N) and sulphur (S) compounds and their long-range transport have caused widespread negative impacts on different ecosystems. Critical loads (CLs) are deposition thresholds used to describe the sensitivity of ecosystems to atmospheric deposition. The CL methodology has been a key science-based tool for assessing the environmental consequences of air pollution. We computed CLs for eutrophication and acidification using a European long-term dataset of intensively studied forested ecosystem sites (n = 17) in northern and central Europe. The sites belong to the ICP IM and eLTER networks. The link between the site-specific calculations and time-series of CL exceedances and measured site data was evaluated using long-term measurements (1990-2017) for bulk deposition, throughfall and runoff water chemistry. Novel techniques for presenting exceedances of CLs and their temporal development were also developed. Concentrations and fluxes of sulphate, total inorganic nitrogen (TIN) and acidity in deposition substantially decreased at the sites. Decreases in S deposition resulted in statistically significant decreased concentrations and fluxes of sulphate in runoff and decreasing trends of TIN in runoff were more common than increasing trends. The temporal developments of the exceedance of the CLs indicated the more effective reductions of S deposition compared to N at the sites. There was a relation between calculated exceedance of the CLs and measured runoff water concentrations and fluxes, and most sites with higher CL exceedances showed larger decreases in both TIN and H+ concentrations and fluxes. Sites with higher cumulative exceedance of eutrophication CLs (averaged over 3 and 30 years) generally showed higher TIN concentrations in runoff. The results provided evidence on the link between CL exceedances and empirical impacts, increasing confidence in the methodology used for the European-scale CL calculations. The results also confirm that emission abatement actions are having their intended effects on CL exceedances and ecosystem impacts.

Meta-analysis of multidecadal biodiversity trends in Europe.

Local biodiversity trends over time are likely to be decoupled from global trends, as local processes may compensate or counteract global change. We analyze 161 long-term biological time series (15-91 years) collected across Europe, using a comprehensive dataset comprising ~6,200 marine, freshwater and terrestrial taxa. We test whether (i) local long-term biodiversity trends are consistent among biogeoregions, realms and taxonomic groups, and (ii) changes in biodiversity correlate with regional climate and local conditions. Our results reveal that local trends of abundance, richness and diversity differ among biogeoregions, realms and taxonomic groups, demonstrating that biodiversity changes at local scale are often complex and cannot be easily generalized. However, we find increases in richness and abundance with increasing temperature and naturalness as well as a clear spatial pattern in changes in community composition (i.e. temporal taxonomic turnover) in most biogeoregions of Northern and Eastern Europe.

Major disturbances test resilience at a long-term boreal forest monitoring site.

The impact of disturbances on boreal forest plant communities is not fully understood, particularly when different disturbances are combined, and regime shifts to alternate stable states are possible after disturbance. A long-term monitored semi-natural forest site subject to intense combined storm and bark beetle damage beginning in 2005 provided an opportunity to investigate the postdisturbance development of the vegetation community. Previous studies suggest that a shift from Picea abies to Fagus sylvatica domination was possible.We analyzed pre- and postdisturbance vegetation data to investigate to what extent vascular plant species abundances, diversity, traits, and community composition have changed. We were particularly interested in differences between remaining apparently unaffected areas (potential refugia) and disturbed areas, and in signs of consistent change over time in community composition in response to disturbance that could indicate an impending regime shift.We found that the vegetation community present in the refuge areas has remained substantially intact through the period of disturbance. Nonrefuge areas diverged from the refuges in community composition and showed increased taxonomic and functional diversity. Despite this, and an increase in deciduous tree species (particularly F. sylvatica), P. abies has shown strong postdisturbance regeneration. The refuges may be important in the apparent ongoing recovery of the disturbed areas to a P. abies-dominated state similar to that found predisturbance. This fast recovery is interpreted as evidence of a system resilient to a potential shift to a deciduous-dominated state. Synthesis: Our results show that even powerful combined disturbances in a system with multiple stable states can be insufficient to initiate a regime shift. Resilience of the P. abies-dominated forest community is increased by the survival of refuge areas functioning as a form of ecological memory of the previous ecosystem state. The results also demonstrate the value of data generated by long-term monitoring programs.

Long-term effects on nitrogen and benthic fauna of extreme weather events: Examples from two Swedish headwater streams.

Climate change is expected to cause an increased frequency of extreme events such as heavy floods and major storms. Such stochastic events have an immediate impact on surface water quality, but the long-term effects are largely unknown. In this study, we assess long-term monitoring data from two Swedish headwater catchments affected by extreme weather events. At one site, where nitrogen effects in soil water, groundwater, and stream water were studied after storm-felling and subsequent forest dieback from bark beetle attack, long-term (> 5 years) but relatively modest (generally <1 mg L⁻¹) increases in ammonium (NH(4)-N) and nitrate (NO(3)-N) concentrations were observed in the various aqueous media. At the other site, where effects on benthic fauna were studied in a stream impacted by extreme geophysical disturbances caused by rainstorm-induced flashflood, only short-term (1 year) effects were revealed both regarding diversity and composition of species.

Forest floor vegetation response to nitrogen deposition in Europe.

Chronic nitrogen (N) deposition is a threat to biodiversity that results from the eutrophication of ecosystems. We studied long-term monitoring data from 28 forest sites with a total of 1,335 permanent forest floor vegetation plots from northern Fennoscandia to southern Italy to analyse temporal trends in vascular plant species cover and diversity. We found that the cover of plant species which prefer nutrient-poor soils (oligotrophic species) decreased the more the measured N deposition exceeded the empirical critical load (CL) for eutrophication effects (P = 0.002). Although species preferring nutrient-rich sites (eutrophic species) did not experience a significantly increase in cover (P = 0.440), in comparison to oligotrophic species they had a marginally higher proportion among new occurring species (P = 0.091). The observed gradual replacement of oligotrophic species by eutrophic species as a response to N deposition seems to be a general pattern, as it was consistent on the European scale. Contrary to species cover changes, neither the decrease in species richness nor of homogeneity correlated with nitrogen CL exceedance (ExCLemp N). We assume that the lack of diversity changes resulted from the restricted time period of our observations. Although existing habitat-specific empirical CL still hold some uncertainty, we exemplify that they are useful indicators for the sensitivity of forest floor vegetation to N deposition.

454 pyrosequencing analysis on faecal samples from a randomized DBPC trial of colicky infants treated with Lactobacillus reuteri DSM 17938.

OBJECTIVE: To analyze the global microbial composition, using large-scale DNA sequencing of 16 S rRNA genes, in faecal samples from colicky infants given L. reuteri DSM 17938 or placebo. METHODS: Twenty-nine colicky infants (age 10-60 days) were enrolled and randomly assigned to receive either Lactobacillus reuteri (10(8) cfu) or a placebo once daily for 21 days. Responders were defined as subjects with a decrease of 50% in daily crying time at day 21 compared with the starting point. The microbiota of faecal samples from day 1 and 21 were analyzed using 454 pyrosequencing. The primers: Bakt_341F and Bakt_805R, complemented with 454 adapters and sample specific barcodes were used for PCR amplification of the 16 S rRNA genes. The structure of the data was explored by using permutational multivariate analysis of variance and effects of different variables were visualized with ordination analysis. RESULTS: The infants' faecal microbiota were composed of Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the four main phyla. The composition of the microbiota in infants with colic had very high inter-individual variability with Firmicutes/Bacteroidetes ratios varying from 4000 to 0.025. On an individual basis, the microbiota was, however, relatively stable over time. Treatment with L. reuteri DSM 17938 did not change the global composition of the microbiota, but when comparing responders with non-responders the group responders had an increased relative abundance of the phyla Bacteroidetes and genus Bacteroides at day 21 compared with day 0. Furthermore, the phyla composition of the infants at day 21 could be divided into three enterotype groups, dominated by Firmicutes, Bacteroidetes, and Actinobacteria, respectively. CONCLUSION: L. reuteri DSM 17938 did not affect the global composition of the microbiota. However, the increase of Bacteroidetes in the responder infants indicated that a decrease in colicky symptoms was linked to changes of the microbiota. TRIAL REGISTRATION: ClinicalTrials.gov NCT00893711.

Epiphytic algae and lichen cover in boreal forests-a long-term study along a N and S deposition gradient in Sweden.

The aim was to describe spatiotemporal patterns of colonization of spruce branches by algae and lichens and the relationship with decreasing deposition of N and S. Coverage was estimated annually over 10 years for four Swedish Integrated Monitoring catchments with varying deposition levels. Initial hypotheses were that algal coverage would be positively correlated with deposition and that lichen coverage would be negatively correlated with S and positively with N deposition. Data were analyzed using regression, ANOVA, and partial least square regression. The results showed a temporal decrease in the coverage of algae but an increase in colonization rates, while lichens showed less uniform patterns. Within catchments, algae and lichen coverages were positively correlated with mainly S deposition. Across catchments, coverage of algae increased, while the coverage of lichens decreased with increasing N and S deposition. Colonization rates of both algae and lichens showed weak correlations with both spatial and temporal trends in N and S deposition. Thus, while N and S deposition had an effect on the colonization and coverage of algae and lichens, other factors are also important.

Understorey vegetation stability and dynamics in unmanaged boreal forests along a deposition gradient in Sweden.

The aims of this study were to investigate spatial patterns and temporal changes in understorey vegetation at four forest catchments forming a depositional gradient. Inventories of the bottom and field layers were carried out in the 1990s and repeated after 5-14 years, depending on catchment. It was hypothesized that changes and patterns in ground vegetation would be related to changes and patterns in N and S deposition. The data were analyzed using Ellenberg indices and multivariate methods. All catchments showed temporal changes in species composition. Analyses of the bottom layer were confounded by a change of field staff, but after accounting for this observer effect, differences in species composition between the catchments remained. Within catchments, the changes in species composition were unrelated to N or S deposition. Relationships between environmental factors, expressed as Ellenberg indices, and compositional patterns differed between catchments although Ellenberg indices showed small temporal changes.

Biodiversity differences between managed and unmanaged forests: meta-analysis of species richness in Europe.

Past and present pressures on forest resources have led to a drastic decrease in the surface area of unmanaged forests in Europe. Changes in forest structure, composition, and dynamics inevitably lead to changes in the biodiversity of forest-dwelling species. The possible biodiversity gains and losses due to forest management (i.e., anthropogenic pressures related to direct forest resource use), however, have never been assessed at a pan-European scale. We used meta-analysis to review 49 published papers containing 120 individual comparisons of species richness between unmanaged and managed forests throughout Europe. We explored the response of different taxonomic groups and the variability of their response with respect to time since abandonment and intensity of forest management. Species richness was slightly higher in unmanaged than in managed forests. Species dependent on forest cover continuity, deadwood, and large trees (bryophytes, lichens, fungi, saproxylic beetles) and carabids were negatively affected by forest management. In contrast, vascular plant species were favored. The response for birds was heterogeneous and probably depended more on factors such as landscape patterns. The global difference in species richness between unmanaged and managed forests increased with time since abandonment and indicated a gradual recovery of biodiversity. Clearcut forests in which the composition of tree species changed had the strongest effect on species richness, but the effects of different types of management on taxa could not be assessed in a robust way because of low numbers of replications in the management-intensity classes. Our results show that some taxa are more affected by forestry than others, but there is a need for research into poorly studied species groups in Europe and in particular locations. Our meta-analysis supports the need for a coordinated European research network to study and monitor the biodiversity of different taxa in managed and unmanaged forests.