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J Appl Microbiol ; 108(3): 1023-1031, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19761463

ABSTRACT

AIMS: To assess the presence of viral pathogens in bathing water samples and to evaluate the interdependency of bacterial indicator counts and viral detection. METHODS AND RESULTS: Bathing water samples of 16 beaches collected along a Portuguese Coastal area were screened for the hepatitis A virus (HAV) and norovirus genogroup I (NVGI) using RT-PCR technique. Bacteriological water quality was also assessed, according to European regulations. HAV and NVGI were detected in 95% and 27% of the water samples, respectively, whereas bacteriological quality was good in all but one sample, according to current water quality regulations. CONCLUSIONS: All water samples would be considered of excellent quality according to the most recent European regulations. No relationship between viral detection and regulatory-based bacterial indicators was found. SIGNIFICANCE AND IMPACT OF THE STUDY: The current results reinforce the importance of increased surveillance for pathogenic viruses in bathing waters.


Subject(s)
Bacteria/isolation & purification , Hepatitis A virus/isolation & purification , Norovirus/isolation & purification , Seawater/microbiology , Seawater/virology , Water Microbiology , Bathing Beaches , Environmental Monitoring , Portugal , RNA, Viral , Reverse Transcriptase Polymerase Chain Reaction , Water Pollutants/isolation & purification
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