Blind adaptive filtering for non-invasive extraction of the fetal electrocardiogram and its non-stationarities.

The objective is to extract automatically a beat-to-beat fetal electrocardiogram (fECG) from a maternal electrocardiogram (mECG) using surface electrodes placed on the maternal abdomen and to derive fetal PR, QT, QTc, and QS durations to allow early diagnosis and monitoring treatment of certain fetal cardiac disorders. mECG and abdominal noise in abdominal maternal recordings can be orders of magnitude stronger than the fECG signal and the P and T waves that are embedded in them. A two-stage blind adaptive filtering algorithm was used for fECG extraction, the first stage using frequency-domain electrocardiogram features and the second considering time-domain features. Three channels of abdominal recordings were obtained from 12 patients at 20-40 weeks of gestation. In each case beat-to-beat unaveraged fECGs were isolated. The combined filter allowed identification of diagnostically important PR, QT, and RR durations. Comparison with synthetic data is also included.

Trisomy 21. Second-trimester ultrasound.

Not every aspect of sonographic examination reveals karyotypic abnormalities. Ultrasound examination of a fetus with trisomy 21 generally reveals normal amniotic fluid, normal placentation, and normal fetal growth. In addition, other chromosomal abnormalities have many of the same sonographic findings as Down syndrome, and many findings have a large overlap with phenotypically normal fetuses. The importance of second-trimester ultrasound screening for Down syndrome has remained great because of its ease of use and relative effectiveness. Trained sonographers can adjust the relative risk for trisomy 21 and alter the need for genetic amniocentesis. It is important that parents understand the limitations of a screening test and the risks and benefits of possible subsequent confirmatory testing. If a major structural abnormality is identified on ultrasound, karyotype determination should be considered. Nuchal thickness in the first or second trimester remains the most clinically useful marker for trisomy 21. The predictive value of all the markers depends on the population studied and can be modified by a host of biochemical markers and historical factors. If fetal karyotype analysis could be performed without sampling through the uterus, prenatal diagnosis could be offered to all pregnant women, and screening would be unnecessary. Despite its limitations, ultrasound will have an important role in prenatal diagnosis at least until isolating and testing fetal cells from maternal blood or other sources becomes practical and widely available. Whether used alone or in conjunction with additional biochemical or molecular serum markers, ultrasound is an important and powerful tool in prenatal genetic evaluation.

X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates.

We determined the structures of Acanthamoeba profilin I and profilin II by x-ray crystallography at resolutions of 2.0 and 2.8 A, respectively. The polypeptide folds and the actin-binding surfaces of the amoeba profilins are very similar to those of bovine and human profilins. The electrostatic potential surfaces of the two Acanthamoeba isoforms differ. Two areas of high positive potential on the surface of profilin II are candidate binding sites for phosphatidylinositol phosphates. The proximity of these sites to the actin binding site provides an explanation for the competition between actin and lipids for binding profilin.