ABSTRACT
We report the draft genome sequence of Turicella otitidis strain TD1, isolated from a central line catheter sample from a patient with a history of bowel obstruction. It contained several genetic determinants of multidrug-resistant phenotypes such as a cfrA 50S methyltransferase, two major facilitator superfamily-type drug resistance transporters, and a putative beta-lactamase.
ABSTRACT
We report the development of a single-tube, multi-locus variable number tandem repeat analysis (MLVA) assay for simultaneous speciation and strain typing of Brucella, the etiologic agent of brucellosis. Our MLVA assay consists of eight loci, two of which are species-specific markers that allow for definitive identification of Brucella melitensis, B. abortus, and Brucella species while ruling out related pathogenic bacterial genera. The remaining six loci are moderately variable loci capable of discriminating between Brucella strains originating within our study area. We applied the assay to a collection of 110 B. melitensis isolates of primarily Mexican origin and to smaller sample sizes of four other Brucella species for a total of 161 isolates. Simpson's index of diversity was 0.985 for B. melitensis and 0.938 for B. abortus. The assay accurately distinguished seven epidemiologically-linked clusters of B. melitensis infections and ascertained the source of infection in several laboratory-acquired cases. This assay is accessible to limited-resource settings due to its technological and economical feasibility. The timely and accurate information provided by this assay will potentially aid brucellosis control efforts, improve patient outcomes, and reduce the occurrence of laboratory-acquired infections.
Subject(s)
Bacterial Typing Techniques/methods , Brucella melitensis/isolation & purification , Brucellosis/microbiology , Polymerase Chain Reaction/methods , Brucella melitensis/classification , Brucella melitensis/genetics , Humans , Minisatellite Repeats , PhylogenyABSTRACT
We report the second human case of infection caused by an organism identified as the proposed Bartonella species, "B. washoensis." The organism was isolated from a blood sample from a patient presenting with meningitis and early sepsis. Oropsylla montana fleas were implicated as the vector for disease transmission in this case.
Subject(s)
Bartonella Infections/diagnosis , Bartonella/isolation & purification , Meningitis, Bacterial/diagnosis , Animals , Bacteremia/microbiology , Bartonella/classification , Bartonella Infections/microbiology , Blood/microbiology , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Disease Vectors , Female , Humans , Meningitis, Bacterial/microbiology , Middle Aged , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Siphonaptera/microbiologyABSTRACT
We reviewed clinical and epidemiologic features of 56 human Capnocytophaga canimorsus isolates submitted during a 32-year period to California's Microbial Diseases Laboratory for identification. An increasing number of isolates identified as C. canimorsus have been submitted since 1990. Many laboratories still have difficulty correctly identifying this species.
Subject(s)
Capnocytophaga/isolation & purification , Gram-Negative Bacterial Infections/diagnosis , Gram-Negative Bacterial Infections/epidemiology , Adolescent , Adult , Aged , Aged, 80 and over , Bacterial Typing Techniques , Capnocytophaga/classification , Capnocytophaga/genetics , Child , Child, Preschool , DNA, Bacterial/analysis , Female , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/physiopathology , Humans , Infant , Male , Middle Aged , Polymerase Chain Reaction , RNA, Ribosomal, 16S/genetics , Risk Factors , Sequence Analysis, DNAABSTRACT
The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.
