Untangling biomechanical differences in perturbation-induced stepping strategies for lateral balance stability in older individuals.

When recovering balance from a lateral perturbation, younger adults tend to stabilize balance with a single lateral sidestep while older adults often take multistep responses. Using multiple steps to recover balance is consistently associated with increased fall risk, altered body center of mass (CoM) control and instability. The aim of this study was to compare the spatio-temporal stepping characteristics and the margin of stability (MoS) of single lateral sidesteps (LSS1) with the first and second steps of a two-step protective step sequence. Two-step sequences begin with either a cross-over step to the front or back, or a medial step followed by a lateral sidestep. Seventy-one older adults received random lateral waist-pull perturbations to either side. We hypothesized that LSS1 would be more stable (larger MoS) than either step in a two-step sequence. With some exceptions, utilizing a two-step sequence was associated with a reduced CoM velocity and distance between the base of support and CoM and decreased stability in the frontal plane following limb loading of the first and second step. There were no differences in the time available to arrest the extrapolated CoM at the end of a single lateral sidestep or the final step of a two-step sequence. Two-step sequences involving a cross-over step include more complex stepping trajectories and also challeng stability in the sagittal plane requiring a multidimensional balance correction. These results indicate important step type differences in center of mass control in recovering balance with a single lateral sidestep as opposed to a two-step sequence among older adults.

Patterns of muscle coordination during stepping responses post-stroke.

This study compared self-induced stepping reactions of seventeen participants after stroke and seventeen controls. Surface electromyographic (EMG) signals were recorded bilaterally from the soleus (SOL), tibialis anterior (TA), biceps femoris (BF) and rectus femoris (RF) muscles. Principal component analysis (PCA) was used to reduce the data into muscle activation patterns and examine group differences (paretic, non-paretic, control leg). The first principal component (PC1) explained 46.7% of the EMG signal of the stepping leg. Two PCs revealed distinct activation features for the stepping paretic leg: earlier TA onset at step initiation and earlier BF and SOL onset at mid-step. For the stance leg, PC1 explained 44.4% of the EMG signal and significant differences were found in the non-paretic leg compared to paretic (p < 0.001) and control (p < 0.001). In PC1, at step onset the BF and SOL EMG and the RF and TA EMG were increased over the latter half of the step. No PC loadings were distinct for the paretic leg during stance, however differences were found in the non-paretic leg: earlier TA burst and increased BF and SOL EMG at step initiation. The results suggest impairments in the paretic leg when stepping and compensatory strategies in the non-paretic stance leg.

Peptones from diverse sources: pivotal determinants of bacterial growth dynamics.

AIMS: In view of the major problems encountered by microbiologists in obtaining reproducible data on growth dynamics in complex media, we studied the effects of different peptones made from different biological sources and produced by numerous manufacturers. METHODS AND RESULTS: Peptones (including casein, gelatin, meat, soy and yeast) were assessed as a constituent of the pre-enrichment broth buffered peptone water (BPW). Generation times (g) and yields of Salmonella serovar Typhimurium were significantly affected by the type of peptone employed with yeast peptones generating yields of 7.04 x 10(9) CFU ml(-1) and gelatin peptones producing 0.81 x 10(9) CFU ml(-1). Medium sterilization was also found to have significant effects (P = 0.000) upon subsequent bacterial growth. Filter sterilization of BPW media produced lower generation times compared with those obtained after sterilization by autoclaving. Finally, it was observed that some peptones which produced good growth when inoculated with healthy organisms, showed relatively poor growth when inocula were sublethally injured by heating. CONCLUSIONS: Variation in peptone as a constituent of BPW has a significant effect on growth and enumeration of bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: Increased consideration with respect to culture media may significantly improve bacterial growth and experimental reproducibility.

Efficacy of a hydroxynaphthoquinone, 566C80, in experimental Pneumocystis carinii pneumonitis.

The efficacy of a new class of drugs for Pneumocystis carinii pneumonitis was demonstrated. 566C80, a hydroxynaphthoquinone, administered orally in a dose of greater than or equal to 100 mg/kg of body weight per day prophylactically prevented P. carinii pneumonitis in 90% or more of rats, while all untreated control animals developed pneumonitis. When 566C80 (100 mg/kg per day) was administered for 3 weeks after P. carinii pneumonitis was established, therapy was totally effective and all of the untreated controls had progressive P. carinii pneumonitis. A dose of 566C80 of between 25 and 50 mg/kg per day protected 50% of the rats from P. carinii pneumonitis, and a dose of between 50 and 100 mg/kg per day cured 50% of those treated for P. carinii pneumonitis. Both prophylaxis and treatment with 566C80 were at least as effective as with trimethoprim-sulfamethoxazole. Animals maintained on immunosuppression after completion of treatment remained free of P. carinii, suggesting a killing effect. Clearance of P. carinii was associated with levels of 60 micrograms or more of 566C80 per ml of plasma. This hydroxynaphthoquinone offers promise as an anti-P. carinii drug.

Shoulder Antagonistic Strength Ratios: A Comparison between College-Level Baseball Pitchers and Nonpitchers.

The purpose of this study was to investigate the shoulder strength ratios obtained from college-level baseball pitchers and age- and sex-matched nonpitchers. Shoulder flexion/extension and externallinternal rotation strength ratios were assessed in 10 pitchers and 9 nonpitchers. Speeds selected for testing were 180 and 300 degrees /sec on the Cybex /I.@R esults indicated that both pitchers and nonpitchers generated greater peak torque values for the extensors and internal rotators than for the flexors and external rotators of the shoulder. A comparison of shoulder strength ratios between a pitcher's throwing arm and his nonthrowing arm was statistically significant (p < 0.05) for shoulder external/internal rotation at the speeds of 180 and 300 "/set. A comparison of shoulder strength ratios between pitchers and nonpitchers on the nondominant arm was not statistically significant for any of the speeds or directions tested. A comparison of the shoulder strength ratios between pitchers and nonpitchers on the dominant arm was statistically significant (p < 0.05) for all directions and speeds tested. The relationship between shoulder muscle imbalance and injury was discussed. J Orthop Sports Phys Ther 1987;8(9):451-461.