Profile of otitis externa isolates in dogs and cats diagnosed in laboratory of microbiology in 2020 / Perfil dos isolados de otites externas em cães e gatos diagnosticados em laboratório de microbiologia em 2020

Otitis externa is one of the most frequent diseases in the clinical routine of dogs and cats, and they can be caused by several associated factors. Due to incorrect use of antimicrobial products, the treatment and control of otitis have become challenging. This study aims to analyze the results of otological exams at the Laboratory of Microbiology HV-ULBRA in 2020 and demonstrate the profile of patients and isolated bacteria. Staphylococcus was the main genus isolated, and 71,11% of samples showed multi-drug resistance to antimicrobial testing. These results indicate the need to use complementary examinations to control otitis externa.(AU)

A otite externa é um das enfermidades mais frequentes na rotina clínica de cães e gatos e pode ser causada por diversos fatores associados. Devido ao uso incorreto de antimicrobianos, o tratamento e o controle das otites se tornaram desafiadores. O objetivo desse estudo é analisar os resultados dos exames otológicos encaminhados ao Laboratório de Microbiologia HV-ULBRA em 2020 e, além disso, delinear o perfil dos pacientes e das bactérias isoladas. Staphylococcus foi o principal gênero isolado e 71,11% das amostras apresentou multirresistência aos antimicrobianos testados. Esses resultados evidenciam a necessidade do uso de exames complementares para controle das otites externas.(AU)

Characterization of Staphylococcus species isolated in production stages of brazilian colonial cheese

Colonial cheese is a culturally and economically important product from the south of Brazil. As most of its production is artisanal, the technology employed is mostly knowledge passed down from one generation to the next according to family tradition and may be produced with raw or pasteurized milk. It is noted for its spicy flavour and variable composition and is often classified as a medium to high-moisture cheese. This intrinsic feature increases the risk of microbial spoilage and food poisoning. One of the main bio-indicators of contamination in colonial cheese is coagulase positive Staphylococcus. The purpose of this study was the phenotypic identification of Staphylococcus species isolated from the products and surfaces in the main production stages of colonial cheese. Staphylococcus sp. isolates from the food and the production environment were obtained from two colonial cheese-production agro-industries in Rio Grande do Sul. Samples of fresh milk, curd, ripening and final colonial cheese were collected. In addition, surface sampling was performed on the coagulation tanks, production tables, molds, cheese ripening shelves and on the hands of the handlers. Staphylococcus sp. isolates in the cheese and the production environments tested in this study were identified by phenotypic techniques through biochemical and MALDI-TOF MS analyses. These isolates were subjected to gene expression analysis for enterotoxins A, B, C, D, and E. All isolates (72) were identified as Staphylococcus sp., and 43% of the total isolates tested were coagulase positive. Staphylococcus aureus was the predominant species in the raw milk and production tanks. Regarding coagulase negative staphylococci isolates, S. warneri and S. sciuri were most abundant. The sea and seb genes were detected in 4% of the Staphylococcus isolates. The results indicate eleven different species of Staphylococcus present in the colonial cheese production environments studied. The predominant presence of S. aureus in the different samples of milk, curd, ripened cheese, ready-to-eat cheese and hands of the handlers indicates that there are issues with the selection of milk-producing animals, pasteurization process and/or hygiene control of handlers. The sea and seb genes were detected in samples of raw milk and colonial cheese. No enterotoxin genes were detected in coagulase negative staphylococci.

Avaliação da contaminação ambiental em Hospital Veterinário e antibiograma acumulativo / Evaluation of environmental contamination at veterinary teaching hospital and cumulative antibiogram

Este artigo aborda inicialmente o controle microbiológico realizado no Hospital Veterinário ULBRA Canoas (HV-ULBRA) no período entre maio de 2016 a agosto de 2017 nos setores como; ambulatórios, internação, centro cirúrgico de pequenos animais de companhia e materiais utilizados na rotina cirúrgica, identificando microrganismos e pontos críticos no ambiente hospitalar. A segunda etapa aborda informações referentes à sensibilidade e resistência de microrganismos a antibióticos, referentes ao sistema otológico, urinário e sistema tegumentar, oriundos de culturas e antibiogramas realizados no período de janeiro a dezembro de 2016 no Laboratório de Microbiologia do HV-ULBRA, abordando o conceito do antibiograma acumulativo, visando maximizar terapias empíricas.(AU)

This paper initially addresses the microbiological control performed at the ULBRA Veterinaryteaching Hospital (HV-ULBRA) in the period between May 2016 to August 2017 in sectors suchas: exam room, wards, surgical center and materials used in the surgical routine, identifyingmicroorganisms and critical points in the hospital environment. The second stage deals with thesensitivity and resistance of microorganisms to antibiotics, referring to the otological, urinary andtegumentary systems, from cultures and antibiograms performed from January to December 2016at the Laboratory of Microbiology of HV-ULBRA. Addrressing the concept of the cumulativeantibiogram, aiming at maximizing empirical therapies.(AU)

Avaliação da contaminação ambiental em Hospital Veterinário e antibiograma acumulativo / Evaluation of environmental contamination at veterinary teaching hospital and cumulative antibiogram

Este artigo aborda inicialmente o controle microbiológico realizado no Hospital Veterinário ULBRA Canoas (HV-ULBRA) no período entre maio de 2016 a agosto de 2017 nos setores como; ambulatórios, internação, centro cirúrgico de pequenos animais de companhia e materiais utilizados na rotina cirúrgica, identificando microrganismos e pontos críticos no ambiente hospitalar. A segunda etapa aborda informações referentes à sensibilidade e resistência de microrganismos a antibióticos, referentes ao sistema otológico, urinário e sistema tegumentar, oriundos de culturas e antibiogramas realizados no período de janeiro a dezembro de 2016 no Laboratório de Microbiologia do HV-ULBRA, abordando o conceito do antibiograma acumulativo, visando maximizar terapias empíricas.

This paper initially addresses the microbiological control performed at the ULBRA Veterinaryteaching Hospital (HV-ULBRA) in the period between May 2016 to August 2017 in sectors suchas: exam room, wards, surgical center and materials used in the surgical routine, identifyingmicroorganisms and critical points in the hospital environment. The second stage deals with thesensitivity and resistance of microorganisms to antibiotics, referring to the otological, urinary andtegumentary systems, from cultures and antibiograms performed from January to December 2016at the Laboratory of Microbiology of HV-ULBRA. Addrressing the concept of the cumulativeantibiogram, aiming at maximizing empirical therapies.