ABSTRACT
ABSTRACT. In order to gather information on the culicid fauna of Nova Iguaçu Municipal Park, Rio de Janeiro, Brazil, adult and immature stages were collected with the Centers for Disease Control and Prevention miniature light traps, and dippers and suction tubes, respectively. In all, 828 adult and 990 immature specimens were collected belonging to 12 genera. Among the species collected were Aedes aegypti, Ae. albopictus, Ae. fluviatilis, Ae. scapularis, Haemagogus leucocelaenus, and Psorophora ferox that are considered of potential medical importance. Culicids used a variety of larval habitats and bred under diverse ecological conditions, mostly in natural water containers formed by bamboo, bromeliad, ground depression, rock pool, stream, tree hole, and in artificial containers such as abandoned bathtub, car carcass, abandoned sink, plastic cup, waste tire, and water tank. Species richness and diversity increased from lower to higher forest cover and was highest in sites with highest diversity and high number of larval habitats.
Subject(s)
Biota , Culicidae/classification , Culicidae/physiology , Ecosystem , Animals , Brazil , Culicidae/growth & development , Larva/classification , Larva/growth & development , Larva/physiologyABSTRACT
The record of Pseudolynchia canariensis on two Rufous Crab-Hawk in situ taken care of the Hospital Veterinarian of the RioZoo Foundation. The nineteen collected specimens had been identified in the Laboratory of Diptera, Fundação Instituto Oswaldo Cruz. The findings of P. canariensis out natural host (Columba livia) it represents a contribution to the studies of the family Hippoboscidae considering that it does not have records about native birds of the american continent parasitized by P. canariensis.
Subject(s)
Diptera/physiology , Hawks/parasitology , Animals , BrazilABSTRACT
We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 microM), a specific inhibitor of chymotrypsin-like serine proteases.
Subject(s)
Diptera/enzymology , Serine Endopeptidases/metabolism , Animals , Diptera/growth & development , Electrophoresis, Polyacrylamide Gel , Larva/enzymology , Serine Endopeptidases/isolation & purificationABSTRACT
We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases.
Subject(s)
Animals , Diptera/enzymology , Serine Endopeptidases/metabolism , Diptera/growth & development , Electrophoresis, Polyacrylamide Gel , Larva/enzymology , Serine Endopeptidases/isolation & purificationABSTRACT
Registro de Pseudolynchia canariensis em dois gaviões caranguejeiros de vida livre atendidos no Hospital Veterinário da Fundação RioZoo. Os dezenoves exemplares coletados foram identificados no Laboratório de Diptera da Fundação Instituto Oswaldo Cruz. O encontro de P. canariensis fora do hospedeiro natural (Columba livia), representa uma contribuição aos estudos da família Hippoboscidae, visto que não há registros sobre aves nativas do continente americano parasitadas por P. canariensis.
The record of Pseudolynchia canariensis on two Rufous Crab-Hawk in situ taken care of the Hospital Veterinarian of the RioZoo Foundation. The nineteen collected specimens had been identified in the Laboratory of Diptera, Fundação Instituto Oswaldo Cruz. The findings of P canariensis out natural hosty (Columba livia) it represents a contribution to the studies of the family Hippoboscidae considering that it does not have records about native birds of the american continent parasitized by P canariensis.
Subject(s)
Animals , Diptera/physiology , Hawks/parasitology , BrazilABSTRACT
Verificou-se a ocorrência de insetos associados a rações industriais para cães e gatos. Coletas simultâneas de péletes foram realizadas após a abertura dos sacos embalados na indústria e distribuídos para comercialização, no período de maio à novembro de 2003. Foram selecionadas para análise, seis diferentes tipos do total das marcas disponíveis para a venda no comércio da cidade do Rio de Janeiro. Registrou-se a incidência dos besouros Necrobia rufipes e Oryzaephilus surinamensis.
