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1.
Cell Death Differ ; 29(11): 2123-2136, 2022 11.
Article in English | MEDLINE | ID: mdl-35449212

ABSTRACT

Mutations in presenilin 1 and 2 (PS1 and PS2) cause autosomal dominant familial Alzheimer's disease (FAD). Ferroptosis has been implicated as a mechanism of neurodegeneration in AD since neocortical iron burden predicts Alzheimer's disease (AD) progression. We found that loss of the presenilins dramatically sensitizes multiple cell types to ferroptosis, but not apoptosis. FAD causal mutations of presenilins similarly sensitizes cells to ferroptosis. The presenilins promote the expression of GPX4, the selenoprotein checkpoint enzyme that blocks ferroptosis by quenching the membrane propagation of lethal hydroperoxyl radicals. Presenilin γ-secretase activity cleaves Notch-1 to signal LRP8 expression, which then controls GPX4 expression by regulating the supply of selenium into the cell since LRP8 is the uptake receptor for selenoprotein P. Selenium uptake is thus disrupted by presenilin FAD mutations, suppressing GPX4 expression. Therefore, presenilin mutations may promote neurodegeneration by derepressing ferroptosis, which has implications for disease-modifying therapeutics.


Subject(s)
Alzheimer Disease , Ferroptosis , Selenium , Humans , Alzheimer Disease/genetics , Alzheimer Disease/metabolism , Amyloid Precursor Protein Secretases/genetics , Amyloid Precursor Protein Secretases/metabolism , Ferroptosis/genetics , Mutation/genetics , Presenilin-1/genetics , Presenilin-1/metabolism , Presenilins/metabolism
2.
Essays Biochem ; 65(7): 925-940, 2021 12 22.
Article in English | MEDLINE | ID: mdl-34623415

ABSTRACT

Ferroptosis is an iron- and lipid peroxidation-dependent cell death modality and emerging evidence indicates that ferroptosis has great explanatory potential for neuronal loss and associated CNS dysfunction in a range of neurodegenerative diseases (e.g., Alzheimer's, Parkinson's and Huntington's diseases, Motor neuron disease, Friedreich ataxia (FRDA)). Ferroptotic death results from lethal levels of phospholipid hydroperoxides that are generated by iron-dependent peroxidation of polyunsaturated fatty acids (PUFAs), such as arachidonic and adrenic acids, which are conjugated to specific phospholipids (e.g., phosphatidylethanolamines (PEs)). The major cellular protector against ferroptosis is glutathione peroxidase 4 (GPX4), a membrane-associated selenoenzyme that reduces deleterious phospholipid hydroperoxides to their corresponding benign phospholipid alcohols in a glutathione-dependent manner. Other complementary protective systems have also been identified that act to bolster cellular defences against ferroptosis. Many pharmacological modulators of the ferroptosis pathway have been identified, targeting proteins involved in iron homoeostasis and autophagy; the production and detoxification of lipid peroxides, and cyst(e)ine/glutathione metabolism. While a growing number of cell signalling pathways converge to regulate the ferroptosis cascade, an emerging understanding of ferroptosis regulation suggests that the ferroptotic 'tone' of cells can be set by the transcription factor, nuclear factor erythroid 2-related factor 2 (NRF2), which transcriptionally controls many key components of the ferroptosis pathway. In this review, we provide a critical overview of the relationship between ferroptosis and NRF2 signalling. With a focus on the role of ferroptosis in Alzheimer's disease (AD), we discuss how therapeutic modulation of the NRF2 pathway is a viable strategy to explore in the treatment of ferroptosis-driven neurodegeneration.


Subject(s)
Alzheimer Disease , Ferroptosis , Alzheimer Disease/metabolism , Humans , Lipid Peroxidation , Lipid Peroxides , NF-E2-Related Factor 2/metabolism
3.
Stem Cell Res ; 31: 227-230, 2018 08.
Article in English | MEDLINE | ID: mdl-30138848

ABSTRACT

The induced pluripotent stem cell (iPSC) lines UOWi002-A and UOWi003-A were reprogrammed from dermal fibroblasts via mRNA transfection. Dermal fibroblasts from a 56 year old female caucasian familial Alzheimer's disease patient carrying A246E mutation in the PSEN1 gene (familial AD3, autopsy confirmed Alzheimer's disease) and a 75 year old female non-demented control from the same family bearing the wild-type PSEN1 A246 genotype were obtained from the Coriell Institute (AG06848 and AG06846, respectively). The generated iPSCs were characterized and pluripotency was confirmed. The PSEN1 genotype was maintained in both iPSC lines. Resource table.


Subject(s)
Alzheimer Disease/genetics , Induced Pluripotent Stem Cells/metabolism , Presenilin-1/metabolism , Aged , Cell Differentiation , Cell Line , Female , Humans , Middle Aged
4.
NPJ Schizophr ; 2: 16002, 2016.
Article in English | MEDLINE | ID: mdl-27336053

ABSTRACT

Our expression microarray studies showed messenger RNA (mRNA) for solute carrier family 39 (zinc transporter), member 12 (SLC39A12) was higher in dorsolateral prefrontal cortex from subjects with schizophrenia (Sz) in comparison with controls. To better understand the significance of these data we ascertained whether SLC39A12 mRNA was altered in a number of cortical regions (Brodmann's area (BA) 8, 9, 44) from subjects with Sz, in BA 9 from subjects with mood disorders and in rats treated with antipsychotic drugs. In addition, we determined whether inducing the expression of SLC39A12 resulted in an increased cellular zinc uptake. SLC39A12 variant 1 and 2 mRNA was measured using quantitative PCR. Zinc uptake was measured in CHO cells transfected with human SLC39A12 variant 1 and 2. In Sz, compared with controls, SLC39A12 variant 1 and 2 mRNA was higher in all cortical regions studied. The were no differences in levels of mRNA for either variant of SLC39A12 in BA 9 from subjects with mood disorders and levels of mRNA for Slc39a12 was not different in the cortex of rats treated with antipsychotic drugs. Finally, expressing both variants in CHO-K1 cells was associated with an increase in radioactive zinc uptake. As increased levels of murine Slc39a12 mRNA has been shown to correlate with increasing cellular zinc uptake, our data would be consistent with the possibility of a dysregulated zinc homeostasis in the cortex of subjects with schizophrenia due to altered expression of SLC39A12.

5.
Front Aging Neurosci ; 6: 143, 2014.
Article in English | MEDLINE | ID: mdl-25071552

ABSTRACT

Copper is critical for the Central Nervous System (CNS) development and function. In particular, different studies have shown the effect of copper at brain synapses, where it inhibits Long Term Potentation (LTP) and receptor pharmacology. Paradoxically, according to recent studies copper is required for a normal LTP response. Copper is released at the synaptic cleft, where it blocks glutamate receptors, which explain its blocking effects on excitatory neurotransmission. Our results indicate that copper also enhances neurotransmission through the accumulation of PSD95 protein, which increase the levels of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors located at the plasma membrane of the post-synaptic density. Thus, our findings represent a novel mechanism for the action of copper, which may have implications for the neurophysiology and neuropathology of the CNS. These data indicate that synaptic configuration is sensitive to transient changes in transition metal homeostasis. Our results suggest that copper increases GluA1 subunit levels of the AMPA receptor through the anchorage of AMPA receptors to the plasma membrane as a result of PSD-95 accumulation. Here, we will review the role of copper on neurotransmission of CNS neurons. In addition, we will discuss the potential mechanisms by which copper could modulate neuronal proteostasis ("neuroproteostasis") in the CNS with focus in the Ubiquitin Proteasome System (UPS), which is particularly relevant to neurological disorders such as Alzheimer's disease (AD) where copper and protein dyshomeostasis may contribute to neurodegeneration. An understanding of these mechanisms may ultimately lead to the development of novel therapeutic approaches to control metal and synaptic alterations observed in AD patients.

6.
PLoS One ; 8(5): e62811, 2013.
Article in English | MEDLINE | ID: mdl-23667524

ABSTRACT

Dietary copper is essential for multicellular organisms. Copper is redox active and required as a cofactor for enzymes such as the antioxidant Superoxide Dismutase 1 (SOD1). Copper dyshomeostasis has been implicated in Alzheimer's disease. Mutations in the presenilin genes encoding PS1 and PS2 are major causes of early-onset familial Alzheimer's disease. PS1 and PS2 are required for efficient copper uptake in mammalian systems. Here we demonstrate a conserved role for presenilin in dietary copper uptake in the fly Drosophila melanogaster. Ubiquitous RNA interference-mediated knockdown of the single Drosophila presenilin (PSN) gene is lethal. However, PSN knockdown in the midgut produces viable flies. These flies have reduced copper levels and are more tolerant to excess dietary copper. Expression of a copper-responsive EYFP construct was also lower in the midgut of these larvae, indicative of reduced dietary copper uptake. SOD activity was reduced by midgut PSN knockdown, and these flies were sensitive to the superoxide-inducing chemical paraquat. These data support presenilin being needed for dietary copper uptake in the gut and so impacting on SOD activity and tolerance to oxidative stress. These results are consistent with previous studies of mammalian presenilins, supporting a conserved role for these proteins in mediating copper uptake.


Subject(s)
Copper/metabolism , Diet , Insect Proteins/metabolism , Presenilins/metabolism , Animals , Biological Transport , Conserved Sequence , Drosophila melanogaster/metabolism , Gene Knockdown Techniques , Insect Proteins/deficiency , Insect Proteins/genetics , Oxidative Stress , Presenilins/deficiency , Presenilins/genetics , RNA Interference , Superoxide Dismutase/metabolism , Superoxide Dismutase-1
7.
Neurochem Int ; 62(5): 540-55, 2013 Apr.
Article in English | MEDLINE | ID: mdl-22982299

ABSTRACT

Alzheimer's disease is the leading cause of dementia in the elderly and is defined by two pathological hallmarks; the accumulation of aggregated amyloid beta and excessively phosphorylated Tau proteins. The etiology of Alzheimer's disease progression is still debated, however, increased oxidative stress is an early and sustained event that underlies much of the neurotoxicity and consequent neuronal loss. Amyloid beta is a metal binding protein and copper, zinc and iron promote amyloid beta oligomer formation. Additionally, copper and iron are redox active and can generate reactive oxygen species via Fenton (and Fenton-like chemistry) and the Haber-Weiss reaction. Copper, zinc and iron are naturally abundant in the brain but Alzheimer's disease brain contains elevated concentrations of these metals in areas of amyloid plaque pathology. Amyloid beta can become pro-oxidant and when complexed to copper or iron it can generate hydrogen peroxide. Accumulating evidence suggests that copper, zinc, and iron homeostasis may become perturbed in Alzheimer's disease and could underlie an increased oxidative stress burden. In this review we discuss oxidative/nitrosative stress in Alzheimer's disease with a focus on the role that metals play in this process. Recent studies have started to elucidate molecular links with oxidative/nitrosative stress and Alzheimer's disease. Finally, we discuss metal binding compounds that are designed to cross the blood brain barrier and restore metal homeostasis as potential Alzheimer's disease therapeutics.


Subject(s)
Alzheimer Disease/metabolism , Homeostasis , Metals/metabolism , Oxidative Stress , Humans , Nitric Oxide/metabolism
8.
J Neurochem ; 119(1): 220-30, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21797865

ABSTRACT

Impaired metal ion homeostasis causes synaptic dysfunction and treatments for Alzheimer's disease (AD) that target metal ions have therefore been developed. The leading compound in this class of therapeutic, PBT2, improved cognition in a clinical trial with AD patients. The aim of the present study was to examine the cellular mechanism of action for PBT2. We show PBT2 induces inhibitory phosphorylation of the α- and ß-isoforms of glycogen synthase kinase 3 and that this activity is dependent on PBT2 translocating extracellular Zn and Cu into cells. This activity is supported when Aß:Zn aggregates are the source of extracellular Zn and adding PBT2 to Aß:Zn preparations promotes Aß degradation by matrix metalloprotease 2. PBT2-induced glycogen synthase kinase 3 phosphorylation appears to involve inhibition of the phosphatase calcineurin. Consistent with this, PBT2 increased phosphorylation of other calcineurin substrates, including cAMP response element binding protein and Ca²âº/calmodulin-dependent protein kinase. These data demonstrate PBT2 can decrease Aß levels by sequestering the Zn that promotes extracellular formation of protease resistant Aß:Zn aggregates, and that subsequent intracellular translocation of the Zn by PBT2 induces cellular responses with synapto-trophic potential. Intracellular translocation of Zn and Cu via the metal chaperone activity of PBT2 may be an important mechanism by which PBT2 improves cognitive function in people with AD.


Subject(s)
Amyloid beta-Peptides/metabolism , Clioquinol/analogs & derivatives , Glycogen Synthase Kinase 3/metabolism , Metals/metabolism , Molecular Chaperones/metabolism , Alzheimer Disease/drug therapy , Blotting, Western , Calcineurin/metabolism , Calcineurin Inhibitors , Caspase 3/metabolism , Cell Line, Tumor , Clioquinol/pharmacology , Copper/metabolism , Enzyme Inhibitors/pharmacology , Humans , Mass Spectrometry , Matrix Metalloproteinase 2/metabolism , Peptide Hydrolases/metabolism , Phosphorylation/drug effects , Zinc/metabolism
9.
J Biol Chem ; 286(11): 9776-86, 2011 Mar 18.
Article in English | MEDLINE | ID: mdl-21239495

ABSTRACT

Dyshomeostasis of extracellular zinc and copper has been implicated in ß-amyloid aggregation, the major pathology associated with Alzheimer disease. Presenilin mediates the proteolytic cleavage of the ß-amyloid precursor protein to release ß-amyloid, and mutations in presenilin can cause familial Alzheimer disease. We tested whether presenilin expression affects copper and zinc transport. Studying murine embryonic fibroblasts (MEFs) from presenilin knock-out mice or RNA interference of presenilin expression in HEK293T cells, we observed a marked decrease in saturable uptake of radiolabeled copper and zinc. Measurement of basal metal levels in 6-month-old presenilin 1 heterozygous knock-out (PS1(+/-)) mice revealed significant deficiencies of copper and zinc in several tissues, including brain. Copper/zinc superoxide dismutase (SOD1) activity was significantly decreased in both presenilin knock-out MEFs and brain tissue of presenilin 1 heterozygous knock-out mice. In the MEFs and PS1(+/-) brains, copper chaperone of SOD1 (CCS) levels were decreased. Zinc-dependent alkaline phosphatase activity was not decreased in the PS null MEFs. These data indicate that presenilins are important for cellular copper and zinc turnover, influencing SOD1 activity, and having the potential to indirectly impact ß-amyloid aggregation through metal ion clearance.


Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Protein Precursor/metabolism , Copper/metabolism , Presenilin-1/metabolism , Superoxide Dismutase/metabolism , Zinc/metabolism , Alzheimer Disease/genetics , Amyloid/genetics , Amyloid/metabolism , Amyloid beta-Protein Precursor/genetics , Animals , Brain/metabolism , Brain Chemistry/genetics , Embryo, Mammalian/cytology , Embryo, Mammalian/metabolism , Fibroblasts/cytology , Fibroblasts/metabolism , HEK293 Cells , Humans , Mice , Mice, Knockout , Molecular Chaperones/genetics , Molecular Chaperones/metabolism , Presenilin-1/genetics , Superoxide Dismutase/genetics , Superoxide Dismutase-1
10.
Int J Biochem Cell Biol ; 43(1): 146-53, 2011 Jan.
Article in English | MEDLINE | ID: mdl-21034850

ABSTRACT

Copper is a cofactor for many essential enzymes in aerobic organisms. When intracellular copper levels are elevated, the Menkes (ATP7A) P-Type ATPase traffics from the trans-Golgi network (TGN) towards the plasma membrane to facilitate copper efflux. The ADP-ribosylation factor 1 (Arf1) is required for maintenance of Golgi architecture and for vesicular trafficking, including the copper-responsive trafficking of ATP7A. Here we report an ATP7A-independent role of Arf1 in copper homeostasis. Whilst the loss of ATP7A function increased copper levels, RNA interference mediated Arf1 knockdown reduced copper accumulation in HeLa cells as well as in both wild-type and ATP7A-null cultured fibroblasts. Arf1 therefore affected copper levels independently of ATP7A mediated copper efflux. Knockdown of Arf79F, the Drosophila melanogasterArf1 orthologue, also reduced copper accumulation in cultured Drosophila S2 cells, indicating an evolutionarily conserved role for this protein in cellular copper homeostasis. Whereas severe Arf1 inhibition with brefeldin A caused fragmentation and dispersal of the TGN resident protein Golgin 97, the peri-nuclear localisation of the Golgin 97 was retained following Arf1 knockdown, consistent with a moderate reduction in Arf1 activity. Ctr1 levels at the plasma membrane of cultured fibroblast cells were reduced following Arf1 knockdown, indicating an Arf1-dependent trafficking pathway is required for correct distribution of this copper uptake protein. Arf1-dependent trafficking pathways are therefore required for optimal copper uptake efficiency in cultured human and Drosophila cells.


Subject(s)
ADP-Ribosylation Factor 1 , Cation Transport Proteins , Copper , Ion Transport/physiology , RNA Interference/physiology , ADP-Ribosylation Factor 1/genetics , ADP-Ribosylation Factor 1/metabolism , Adenosine Triphosphatases/metabolism , Animals , Brefeldin A/pharmacology , Cation Transport Proteins/genetics , Cation Transport Proteins/metabolism , Cell Membrane/metabolism , Copper/metabolism , Copper-Transporting ATPases , Drosophila , Fibroblasts/metabolism , Gene Expression , Gene Knockdown Techniques , Golgi Apparatus/drug effects , Golgi Apparatus/metabolism , HeLa Cells , Humans
11.
PLoS One ; 5(12): e14303, 2010 Dec 20.
Article in English | MEDLINE | ID: mdl-21188142

ABSTRACT

Copper is essential for aerobic life, but many aspects of its cellular uptake and distribution remain to be fully elucidated. A genome-wide screen for copper homeostasis genes in Drosophila melanogaster identified the SNARE gene Syntaxin 5 (Syx5) as playing an important role in copper regulation; flies heterozygous for a null mutation in Syx5 display increased tolerance to high dietary copper. The phenotype is shown here to be due to a decrease in copper accumulation, a mechanism also observed in both Drosophila and human cell lines. Studies in adult Drosophila tissue suggest that very low levels of Syx5 result in neuronal defects and lethality, and increased levels also generate neuronal defects. In contrast, mild suppression generates a phenotype typical of copper-deficiency in viable, fertile flies and is exacerbated by co-suppression of the copper uptake gene Ctr1A. Reduced copper uptake appears to be due to reduced levels at the plasma membrane of the copper uptake transporter, Ctr1. Thus Syx5 plays an essential role in copper homeostasis and is a candidate gene for copper-related disease in humans.


Subject(s)
Cation Transport Proteins/genetics , Copper/metabolism , Drosophila Proteins/genetics , Qa-SNARE Proteins/metabolism , Animals , Animals, Genetically Modified , Cation Transport Proteins/metabolism , Cell Membrane/metabolism , Copper Transport Proteins , Drosophila Proteins/metabolism , Drosophila melanogaster/genetics , Heterozygote , Homeostasis , Humans , Mammals , Neurons/metabolism , Phenotype , Qa-SNARE Proteins/genetics , RNA Interference
12.
Cell ; 142(6): 857-67, 2010 Sep 17.
Article in English | MEDLINE | ID: mdl-20817278

ABSTRACT

Alzheimer's Disease (AD) is complicated by pro-oxidant intraneuronal Fe(2+) elevation as well as extracellular Zn(2+) accumulation within amyloid plaque. We found that the AD ß-amyloid protein precursor (APP) possesses ferroxidase activity mediated by a conserved H-ferritin-like active site, which is inhibited specifically by Zn(2+). Like ceruloplasmin, APP catalytically oxidizes Fe(2+), loads Fe(3+) into transferrin, and has a major interaction with ferroportin in HEK293T cells (that lack ceruloplasmin) and in human cortical tissue. Ablation of APP in HEK293T cells and primary neurons induces marked iron retention, whereas increasing APP695 promotes iron export. Unlike normal mice, APP(-/-) mice are vulnerable to dietary iron exposure, which causes Fe(2+) accumulation and oxidative stress in cortical neurons. Paralleling iron accumulation, APP ferroxidase activity in AD postmortem neocortex is inhibited by endogenous Zn(2+), which we demonstrate can originate from Zn(2+)-laden amyloid aggregates and correlates with Aß burden. Abnormal exchange of cortical zinc may link amyloid pathology with neuronal iron accumulation in AD.


Subject(s)
Alzheimer Disease/pathology , Amyloid beta-Protein Precursor/antagonists & inhibitors , Amyloid beta-Protein Precursor/metabolism , Ceruloplasmin/antagonists & inhibitors , Zinc/metabolism , Alzheimer Disease/metabolism , Amino Acid Sequence , Amyloid beta-Protein Precursor/chemistry , Animals , Cell Line , Ceruloplasmin/chemistry , Ceruloplasmin/metabolism , Humans , Iron/metabolism , Mice , Sequence Alignment
13.
Proteomics ; 8(9): 1819-31, 2008 May.
Article in English | MEDLINE | ID: mdl-18384105

ABSTRACT

Neurodegenerative illnesses are characterized by aberrant metabolism of biometals such as copper (Cu), zinc (Zn) and iron (Fe). However, little is known about the metabolic effects associated with altered metal homeostasis. In this study, we used an in vitro model of altered Cu homeostasis to investigate how Cu regulates cellular protein expression. Human fibroblasts containing a natural deletion mutation of the Menkes (MNK) ATP7A Cu transporter (MNK deleted) were compared to fibroblasts overexpressing ATP7A (MNK transfected). Cultures of MNK-transfected (Low-Cu) cells exhibited 95% less intracellular Cu than MNK-deleted (High-Cu) cells. Comparative proteomic analysis of the two cell-lines was performed using antibody microarrays, and significant differential protein expression was observed between Low-Cu and High-Cu cell-lines. Western blot analysis confirmed the altered protein expression of Ku80, nexilin, L-caldesmon, MAP4, Inhibitor 2 and DNA topoisomerase I. The top 50 altered proteins were analysed using the software program Pathway Studio (Ariadne Genomics) and revealed a significant over-representation of proteins involved in DNA repair and maintenance. Further analysis confirmed that expression of the DNA repair protein Ku80 was dependent on cellular Cu homeostasis and that Low-Cu levels in fibroblasts resulted in elevated susceptibility to DNA oxidation.


Subject(s)
Adenosine Triphosphatases/genetics , Cation Transport Proteins/genetics , Copper/chemistry , Fibroblasts/metabolism , Adenosine Triphosphatases/chemistry , Adenosine Triphosphatases/metabolism , Antigens, Nuclear/biosynthesis , Biological Transport , Cation Transport Proteins/chemistry , Cation Transport Proteins/metabolism , Computational Biology/methods , Copper-Transporting ATPases , DNA/chemistry , DNA-Binding Proteins/biosynthesis , Humans , Ku Autoantigen , Neurodegenerative Diseases/metabolism , Oligonucleotide Array Sequence Analysis , Oxygen/chemistry , Protein Array Analysis , Proteomics/methods , Software
14.
J Histochem Cytochem ; 56(4): 389-99, 2008 Apr.
Article in English | MEDLINE | ID: mdl-18180385

ABSTRACT

A role for the copper transporter, ATP7B, in secretion of copper from the human breast into milk has previously not been reported, although it is known that the murine ortholog of ATP7B facilitates copper secretion in the mouse mammary gland. We show here that ATP7B is expressed in luminal epithelial cells in both the resting and lactating human breast, where it has a perinuclear localization in resting epithelial cells and a diffuse location in lactating tissue. ATP7B protein was present in a different subset of vesicles from those containing milk proteins and did not overlap with Menkes ATPase, ATP-7A, except in the perinuclear region of cells. In the cultured human mammary line, PMC42-LA, treatment with lactational hormones induced a redistribution of ATP7B from a perinuclear region to a region adjacent, but not coincident with, the apical plasma membrane. Trafficking of ATP7B was copper dependent, suggesting that the hormone-induced redistribution of ATP7A was mediated through an increase in intracellular copper. Radioactive copper ((64)Cu) studies using polarized PMC42-LA cells that overexpressed mAtp7B protein showed that this transporter facilitates copper efflux from the apical surface of the cells. In summary, our results are consistent with an important function of ATP7B in the secretion of copper from the human mammary gland.


Subject(s)
Adenosine Triphosphatases/biosynthesis , Cation Transport Proteins/biosynthesis , Epithelial Cells/metabolism , Hormones/physiology , Lactation/metabolism , Mammary Glands, Human/metabolism , Animals , Cell Line , Cell Line, Tumor , Copper/metabolism , Copper-Transporting ATPases , Female , Hormones/pharmacology , Humans , Immunohistochemistry , In Situ Hybridization , Mammary Glands, Human/cytology , Mice , Milk Proteins/metabolism , Protein Transport
15.
Cell Physiol Biochem ; 20(6): 1073-84, 2007.
Article in English | MEDLINE | ID: mdl-17975309

ABSTRACT

BACKGROUND/AIMS: The copper transporting ATPases, Menkes (ATP7A; MNK) and Wilson (ATP7B; WND) are essential for normal copper transport in the human body. The placenta is the key organ in copper supply to the fetus during pregnancy and it is one of the few organs in the body to express both of the ATPases. The placenta therefore provides a unique opportunity to elucidate the specific roles of these transporters within the one cell type. METHODS/RESULTS: Using polarized placental Jeg-3 cells, siRNA technology and radio-labelled 64Cu transport assays, MNK and WND were shown to have distinct roles in the vectorial transport of copper. MNK transported copper from the cell via the basolateral membrane and in contrast, WND transported copper from the apical membrane. Inactivation of MNK resulted in decreased activity of two important cuproenzymes, lysyl oxidase and Cu/Zn-superoxide dismutase. CONCLUSIONS: Overall, these results provide definitive evidence for distinct roles of MNK and WND in the human placenta, and are consistent with a role for MNK in the transport of copper into the fetal circulation, and through delivery of copper to placental cuproenzymes, whilst WND contributes to the maintenance of placental copper homeostasis by transporting copper to the maternal circulation.


Subject(s)
Adenosine Triphosphatases/metabolism , Cation Transport Proteins/metabolism , Placenta/cytology , Placenta/enzymology , Adenosine Triphosphatases/genetics , Biological Transport , Cation Transport Proteins/genetics , Cell Line , Cell Membrane/metabolism , Cell Polarity , Copper/metabolism , Copper-Transporting ATPases , Female , Gene Expression , Gene Expression Regulation , Humans , Placenta/metabolism , Pregnancy , Protein-Lysine 6-Oxidase/metabolism , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Superoxide Dismutase/metabolism , Transfection
16.
Biochem J ; 402(2): 241-50, 2007 Mar 01.
Article in English | MEDLINE | ID: mdl-17109627

ABSTRACT

Copper deficiency during pregnancy results in early embryonic death and foetal structural abnormalities including skeletal, pulmonary and cardiovascular defects. During pregnancy, copper is transported from the maternal circulation to the foetus by mechanisms which have not been clearly elucidated. Two copper-transporting ATPases, Menkes (ATP7A; MNK) and Wilson (ATP7B; WND), are expressed in the placenta and both are involved in placental copper transport, as copper accumulates in the placenta in both Menkes and Wilson disease. The regulatory mechanisms of MNK and WND and their exact role in the placenta are unknown. Using a differentiated polarized Jeg-3 cell culture model of placental trophoblasts, MNK and WND were shown to be expressed within these cells. Distinct roles for MNK and WND are suggested on the basis of their opposing responses to insulin. Insulin and oestrogen increased both MNK mRNA and protein levels, altered the localization of MNK towards the basolateral membrane in a copper-independent manner, and increased the transport of copper across this membrane. In contrast, levels of WND were decreased in response to insulin, and the protein was located in a tight perinuclear region, with a corresponding decrease in copper efflux across the apical membrane. These results are consistent with a model of copper transport in the placenta in which MNK delivers copper to the foetus and WND returns excess copper to the maternal circulation. Insulin and oestrogen stimulate copper transport to the foetus by increasing the expression of MNK and reducing the expression of WND. These data show for the first time that MNK and WND are differentially regulated by the hormones insulin and oestrogen in human placental cells.


Subject(s)
Adenosine Triphosphatases/metabolism , Cation Transport Proteins/metabolism , Hormones/pharmacology , Placenta/drug effects , Placenta/enzymology , Adenosine Triphosphatases/genetics , Biological Transport , Blotting, Western , Cation Transport Proteins/genetics , Cell Line , Copper/metabolism , Copper-Transporting ATPases , Gene Expression Regulation , Humans , Molecular Sequence Data , Promoter Regions, Genetic/genetics , RNA, Messenger/genetics , Response Elements , Sequence Alignment , Sequence Homology, Nucleic Acid
17.
Biometals ; 17(1): 87-98, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14977365

ABSTRACT

The human X-linked recessive copper deficiency disorder, Menkes disease, is caused by mutations in the ATP7A (MNK) gene, which encodes a transmembrane copper-transporting P-type ATPase (MNK). The MNK protein is localised to the Golgi apparatus and relocalises to the plasma membrane when copper levels are elevated. Previous studies have identified a C-terminal di-leucine endocytic motif (L1487L1488) in MNK, thought to direct it into the clathrin-mediated endocytic pathway. To determine whether MNK is internalised via clathrin-dependent endocytosis, this pathway was blocked in MNK-overexpressing HeLa cells by the transient expression of dominant negative dynamin and Eps15 mutants. MNK internalisation was not inhibited in such cells. MNK internalisation was inhibited in cells treated with hypertonic sucrose that not only blocked clathrin-mediated endocytosis but also fluid-phase endocytosis. These studies, together with earlier studies on the requirement for L1487L1488, suggest that MNK can utilise both clathrin-dependent and clathrin-independent endocytosis in HeLa cells.


Subject(s)
Adenosine Triphosphatases/metabolism , Cation Transport Proteins/metabolism , Copper/metabolism , Endocytosis , Recombinant Fusion Proteins/metabolism , Adenosine Triphosphatases/genetics , Calcium-Binding Proteins/genetics , Calcium-Binding Proteins/metabolism , Cation Transport Proteins/genetics , Clathrin/metabolism , Copper-Transporting ATPases , Dynamin I/chemistry , Dynamin I/genetics , Dynamin I/metabolism , Gene Expression , Genes, Dominant/genetics , HeLa Cells , Humans , Leucine/genetics , Leucine/metabolism , Lysine/genetics , Lysine/metabolism , Mutation/genetics , Protein Transport , Recombinant Fusion Proteins/genetics
18.
Biochem J ; 378(Pt 3): 1031-7, 2004 Mar 15.
Article in English | MEDLINE | ID: mdl-14640979

ABSTRACT

MNK (Menkes copper-translocating P-type ATPase, or the Menkes protein; ATP7A) plays a key role in regulating copper homoeostasis in humans. MNK has been shown to have a dual role in the cell: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper from the cell. These roles can be achieved through copper-regulated trafficking of MNK. It has previously been shown to undergo trafficking from the trans -Golgi network to the plasma membrane in response to elevated copper concentrations, and to be endocytosed from the plasma membrane to the trans -Golgi network upon the removal of elevated copper. However, the fundamental question as to whether copper influences trafficking of MNK to or from the plasma membrane remained unanswered. In this study we utilized various methods of cell-surface biotinylation to attempt to resolve this issue. These studies suggest that copper induces trafficking of MNK to the plasma membrane but does not affect its rate of internalization from the plasma membrane. We also found that only a specific pool of MNK can traffic to the plasma membrane in response to elevated copper. Significantly, copper appeared to divert MNK into a fast-recycling pool and prevented it from recycling to the Golgi compartment, thus maintaining a high level of MNK in the proximity of the plasma membrane. These findings shed new light on the cell biology of MNK and the mechanism of copper homoeostasis in general.


Subject(s)
Adenosine Triphosphatases/metabolism , Cation Transport Proteins/metabolism , Cell Membrane/metabolism , Copper/pharmacology , Adenosine Triphosphatases/physiology , Animals , CHO Cells , Cation Transport Proteins/physiology , Cricetinae , Endocytosis/drug effects , Kinetics , Protein Transport , Recombinant Fusion Proteins/physiology
19.
Biochim Biophys Acta ; 1588(2): 189-94, 2002 Nov 20.
Article in English | MEDLINE | ID: mdl-12385784

ABSTRACT

The Wilson disease (WD) protein (ATP7B) is a copper-transporting P-type ATPase that is responsible for the efflux of hepatic copper into the bile, a process that is essential for copper homeostasis in mammals. Compared with other mammals, sheep have a variant copper phenotype and do not efficiently excrete copper via the bile, often resulting in excessive copper accumulation in the liver. To investigate the function of sheep ATP7B and its potential role in the copper-accumulation phenotype, cDNAs encoding the two forms of ovine ATP7B were transfected into immortalised fibroblast cell lines derived from a Menkes disease patient and a normal control. Both forms of ATP7B were able to correct the copper-retention phenotype of the Menkes cell line, demonstrating each to be functional copper-transporting molecules and suggesting that the accumulation of copper in the sheep liver is not due to a defect in the copper transport function of either form of sATP7B.


Subject(s)
Adenosine Triphosphatases/biosynthesis , Cation Transport Proteins/biosynthesis , Copper/metabolism , Membrane Proteins/biosynthesis , Menkes Kinky Hair Syndrome/metabolism , Adenosine Triphosphatases/genetics , Animals , Blotting, Western , Cation Transport Proteins/genetics , Cells, Cultured , Copper Radioisotopes , Copper Transporter 1 , Copper-Transporting ATPases , Fibroblasts , Humans , Membrane Proteins/genetics , Microscopy, Fluorescence , Sheep , Transfection
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