ABSTRACT
Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.
Subject(s)
Mucoproteins/metabolism , Oligosaccharides/metabolism , Sulfotransferases/metabolism , Animals , Binding Sites , Cell Line , Cricetinae , Glycoside Hydrolases/metabolism , Glycosylation , Humans , In Vitro Techniques , Protein Binding , Protein Conformation , Protein Structure, Tertiary , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Solubility , Staphylococcal Protein A/chemistry , Sulfates/metabolism , Sulfotransferases/chemistry , Sulfotransferases/genetics , Sulfotransferases/isolation & purification , Sulfotransferases/urine , UromodulinABSTRACT
Iminosugars have emerged in the literature during mid-1960's as synthetic compounds. Around the same time, the first examples were found in Nature and their interesting enzyme inhibitory properties were recognised. Due to their powerful interference with glycohydrolases (glycosidases) as well as glycosyltransferases, quite a few representatives exhibit notable biological activities. These range from anti-diabetic, insect antifeedant, nematicidal, plant growth regulating to immunomodulating, anti-cancer as well as - in selected cases - anti-infective properties. The latter will be the focus of the following survey.
