ABSTRACT
The genetic polymorphism of the human Properdin Factor B (BF) in five populations of the Iberian Peninsula (Galicia, Castilla-Leon, Castilla-La Mancha, Extremadura and Western Andalusia) was analysed by means of Isoelectric Focusing in polyacrylamide gels followed by Immunofixation-Silver Staining. Statistical analysis of heterogeneity showed significant differences in the distribution of BF allele frequencies among the Iberian populations so far examined. The high allele frequencies obtained for BF*F and BF*F1 give support to the allelocline distribution hypothesis in the European continent (regression analysis between allele frequency and latitude: r = -0.6237 and r+ -0.8058, for BF*F1 and BF*F respectively).
Subject(s)
Complement Factor B/genetics , Gene Frequency , Phenotype , Racial Groups/genetics , Alleles , Electrophoresis, Polyacrylamide Gel , Genetic Heterogeneity , Humans , Isoelectric Focusing , Polymorphism, Genetic , Regression Analysis , Spain , White People/geneticsABSTRACT
The distribution of Plasminogen (PLG) phenotypes in five populations of Spain (Galicia, Castilla-La Mancha, Castilla-León, Extremadura and Western Andalucia) is analysed by means of polyacrylamide gel isoelectric focusing and immunofixation. PLG*A frequencies, ranging from 0.7281 in Extremadura to 0.8038 in Castilla-León, are similar to those reported for other Southern populations of the European continent and higher than those in Northern and Central European populations. Heterogeneity analysis of the PLG allele distribution among different regions of the Iberian Peninsula have also been carried out.
Subject(s)
Gene Frequency , Plasminogen/genetics , White People/genetics , Alleles , Asian People/genetics , Black People/genetics , Ethnicity/genetics , Genetic Markers , Humans , Phenotype , Portugal , SpainABSTRACT
Immunofixation procedures were used for detecting alpha-1 antitrypsin protease inhibitor (Pi) phenotypes in bloodstains. Neuraminidase elution of bloodstains, together with isoelectric focusing, immunofixation, and silver staining techniques, makes possible Pi subtyping in old bloodstains. No extra bands appear when the storage time is no longer than three months.
Subject(s)
Blood Grouping and Crossmatching/methods , Blood Stains , Protease Inhibitors/blood , Alleles , Blood Preservation , Genetic Variation , Humans , Isoelectric Focusing , PhenotypeABSTRACT
The distribution of the phenotypes for coagulation factor XIIIA subunit (F13A) of autochthonous individuals from the following five Spanish populations was studied: Galicia, Castilla-León, Castilla-La Mancha, Extremadura and Western Andalusia. The frequency values obtained for F13A*2 ranged from 0.248 to 0.311. To date, these values are the highest recorded in the world.
